Jalali Morteza, Saldanha Francesca Y.L., Jalali Mehdi (eds.) Basic Science Methods for Clinical Researchers

Fwd. by Dame Sally Davies. — London : Academic Press, 2017. — 382 p. — ISBN 9780128030783.Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core expermental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data.
This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science.The Polymerase Chain Reaction: PCR, qPCR, and RT-PCR Methods of Cloning
Whole-Mount In Situ Hybridization and a Genotyping Method on Single Xenopus Embryos
Microarrays: An Introduction and Guide to Their Use
Analysis of Human Genetic Variations Using DNA
Sequencing
Western Blot
The Enzyme-linked Immunosorbent Assay: The Application of ELISA in Clinical Research
Immunofluorescence
Cell Culture: Growing Cells as Model Systems in vitro
Flow Cytometry
Transfection
In Vivo Animal Modeling: Drosophila
Zebrafish as a Research Organism: Danio rerio in Biomedical Research
Xenopus as a Model Organism for Biomedical Research
Basic Mouse Methods for Clinician Researchers: Harnessing the Mouse for Biomedical Research
Appendix A. Legal Framework on the Scientific Use of Animals in Research
Appendix B. Regulatory Frameworks for Stem Cell Research
Appendix C. Using Multiple Experimental Methods to Address Basic Science Research Questions