2nd edition. — Humana Press, 2024. — 244 p. — (Methods in Molecular Biology 2722). — ISBN 978-1-0716-3477-6 This second edition provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue. Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization...
2nd edition. — Humana Press, 2024. — 316 p. — (Methods in Molecular Biology 2734). — ISBN 978-1-0716-3523-0. This highly anticipated second edition details cutting-edge experimental approaches for bacteriophage research. This comprehensive volume details new and updated experimental approaches for isolating, formulating, and engineering bacteriophage medicinal products....
2nd edition. — Humana Press, 2023. — 1213 p. — (Methods in Molecular Biology 2654). — ISBN 978-1-0716-3135-5. This new collection features the most up-to-date essential protocols that are currently being used to study the immune synapse. Beginning with methods for making biophysical measurements, the volume continues by covering the cell biology of synapses, methods for...
Humana Press, 2023. — 286 p. — (Methods in Molecular Biology 2770). — ISBN 978-1-0716-3698-5. This detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic...
Humana Press, 2021. — 443 p. — (Methods in Molecular Biology, 2296). — ISBN 978-1-0716-1357-3. Antimicrobial resistance will become a global health threat since antimicrobial treatments continue at the forefront of the defense against microbial infections. To respond to the issue, this detailed book explores vital methodologies currently in use to advance our understanding of...
Humana Press, 2021. — 472 p. — ISBN 978-1-0716-1361-0. This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments...
2nd edition. — Humana, 2021. — 407 p. — (Methods in Molecular Biology, 2222). — ISBN 978-1-0716-0996-5. This fully updated edition explores conceptual as well as technical guidelines for plant taxonomists and geneticists, such as the increasing use of next-generation sequencing (NGS) technologies for numerous applications in plant taxonomy. The volume provides molecular...
Humana Press, 2019. — 1432 p. — (Methods in Molecular Biology 2022) — ISBN: 978-1-4939-9608-7 This volume explores the recent advancements in biomolecular simulations of proteins, small molecules, and nucleic acids, with a primary focus on classical molecular dynamics (MD) simulations at atomistic, coarse-grained, and quantum/ab-initio levels. The chapters in this book are...
Humana Press, 2024. — 379 p. — (Methods in Molecular Biology 2717). — ISBN 978-1-0716-3453-0. This detailed volume provides a comprehensive collection of methods and protocols in food allergy and food allergens studies. The selected protocols explore the study of food allergens, from recombinant production, purification procedures, IgE and T cell epitopes characterization, to...
Humana Press, 2021. — 326 p. — ISBN 978-1-0716-1640-6. This thorough book collects methods and strategies to analyze proteomics data. It is intended to describe how data obtained by gel-based or gel-free proteomics approaches can be inspected, organized, and interpreted to extrapolate biological information. Organized into four sections, the volume explores strategies to...
Humana Press, 2022. — 1081 p. — (Methods in Molecular Biology 2452). — ISBN 978-1-0716-2111-0. This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in...
Humana Press, 2021. — 1058 p. — (Methods in Molecular Biology 2185). — ISBN 978-1-0716-0810-4. The detailed volume aims to provide a comprehensive hands-on manual covering all the techniques involved in the cellular and molecular identification and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human...
Humana Press, 2023. — 423 p. — (Methods in Molecular Biology 2685). — ISBN 978-1-0716-3295-6. This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures...
3rd edition. — Humana Press, 2022. — 905 p. — (Methods in Molecular Biology 2404). — ISBN 978-1-0716-1851-6. This volume presents the most recent advances in techniques for studying the post-transcriptional regulation of gene expression (PTR). With sections on bioinformatics approaches, expression profiling, the protein and RNA interactome, the mRNA lifecycle, and RNA...
Springer, 2021. — 467 p. — (Methods in Molecular Biology). — ISBN 978-1-0716-0198-3. With the advent of so-called “next generation” sequencing technologies, the study of genetic variation in populations gained a new dimension, turning population genetics into population genomics. While a scaling-up in data set size accompanied this shift, population genomics is more than just...
3rd edition. — Humana Press, 2022. — 1173 p. — (Methods in Molecular Biology 2443). — ISBN 978-1-0716-2067-0. This new edition focuses on applied bioinformatics with specific applications to crops, model and diverse plant species. The scope extends from the genome to the phenome and includes aspects of data management, analysis, visualization, and integration. The methods and...
Humana Press, 2020. — 808 p. — (Methods in Molecular Biology 2058). — ISBN: 978-1-4939-9793-0. This book aims to provide a guide for virologists, translational researchers, and clinicians in the field of cancer research by providing reference protocols and methodologies from vector development through clinical translation. Written in the highly successful Methods in Molecular...
Humana Press, 2022. — 287 p. — (Methods in Molecular Biology 2533). — ISBN 978-1-0716-2500-2. This Open Access volume provides comprehensive reviews and describes the latest techniques to study eukaryotic ribosome biogenesis. For more than 50 years ribosomes are a major research topic. Our knowledge about ribosome biogenesis and function such as transcription, mRNA...
Springer, 2017. — 224 p. — (Mvtthods in Molecular Biology 1663). — ISBN10: 1493972642. This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural...
Humana Press, 2018. — 526 p. — (Methods in Molecular Biology 1771). — ISBN 978-1-4939-7792-5. This volume provides a comprehensive look at the current fabrication methods, materials, cell patterning techniques, and practical application of live-cell microarrays. The chapters in this book are categorized into six parts: introduction, biointerface engineering and live-cell...
Humana Press, 2021. — 359 p. — ISBN 978-1-0716-1668-0. This detailed book serves as a systematic examination of the analytical methods to study the transcription factor NF-κB in physiology and disease. It provides an up-to-date guidebook to navigate both conventional and highly specialized methods to detect and analyze the different signaling pathways of NF-κB activation and...
Humana Press, 2023. — 252 p. — (Methods in Molecular Biology 2703). — ISBN 978-1-0716-3389-2. This volume discusses the latest online plant genomics and cytogenetic resources used by plant evolutionary biologists and plant breeders. The chapters in this book are organized into two parts. Part One looks at plant genomic databases, and covers topics such as plant phenomics and...
Humana Press, 2022. — 595 p. — (Methods in Molecular Biology 2380). — ISBN 978-1-0716-1736-6. The volume provides a comprehensive overview of methods that have been developed for the study of Tfh and Tfr biology in mice, and in different human diseases. The chapters detail methods to investigate the function of Tfh cells in vivo and in vitro, including the use of mathematical...
3rd edition. — Humana Press, 2023 — 352 p. — (Methods in Molecular Biology 2664). — ISBN 978-1-0716-3179-9. This third edition provides updated and new chapters on recent innovations in basic renal research. Chapters are divided into three sections covering in vitro, in vivo, and ex vivo models of kidney disease, recent advances in imaging techniques and protocols for...
2nd edition. — Humana Press, 2023. — 321 p. — (Methods in Molecular Biology 2684). — ISBN 978-1-0716-3291-8. This fully updated volume explores recently improved avenues to study urothelial carcinomas. Beginning with several novel chapters on molecular characterization and urothelial carcinogenesis, the book continues with sections on cellular and animal models, biomarkers, and...
Humana Press, 2023. — 327 p. — (Methods in Molecular Biology 2695). — ISBN 978-1-0716-3346-5. This book analyzes liquid biopsy applications in cancer and other diseases. Chapters guide readers through the latest technologies and analysis methods for liquid biopsy,liquid biopsy in cancer, role of liquid biopsies in rheumatoid arthriti, cell-free circulating DNA profiling in...
Humana Press, 2023. — 899 p. — (Methods in Molecular Biology 2660). — ISBN 978-1-0716-3163-8. This thorough volume explores recent advances that have revolutionized the field of precision oncology. The chapters, contributed by experts in the areas of cancer systems and integrative biology, provide hands-on guidance toward developing tools to monitor spatial and temporal changes...
Springer, 2021. — 322 p. — (Methods in Molecular Biology, 2231). — ISBN 978-1-0716-1035-0. This volume is a collection of protocols to install and run tools for calculation and visualiza- tion of multiple sequence alignment (MSA) and other analyses related to MSA. Following the policy of Methods in Molecular Biology, each chapter basically consists of a brief back- ground and a...
Humana Press, 2021. — 330 p. — ISBN 978-1-0716-1440-2. This volume explores the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book are organized into five parts. Part One described...
2nd edition. — Humana Press, 2024 — 434 p. — (Methods in Molecular Biology 2772). — ISBN 978-1-0716-3710-4. This second edition provides new and updated methods detailing techniques and state-of-the-art approaches on the structure and function of plant endoplasmic reticulum (ER). Chapters guide readers through modern microscopy techniques, software protocols, purification, and...
New York: Humana, 2023. — 266 p. This volume provides a comprehensive collection of experimental protocols for investigating ferroptosis in different systems, including cultured cells, animal models, and human tissues. The techniques covered in this book look at various aspects of ferroptosis ranging from the detection of lipid peroxidation to the measurement of glutathione...
Humana Press, 2024. — 230 p. — (Methods in Molecular Biology 2769). — ISBN 978-1-0716-3694-7. This volume discusses the latest advancements in modern methodologies used to study liver carcinogenesis. The first half of this book describes pertinent preclinical models of hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), established either through orthotopic induction...
Humana Press, 2022. — 691 p. — (Methods in Molecular Biology 2523). — ISBN 978-1-0716-2449-4. This volume presents a collection of protocols to study effector-triggered immunity (ETI) in both plants and animals from eminent groups in the field. The chapters in this book cover topics such as genetic manipulation of plant and animal pathogens, host cells, and the analysis of key...
2nd edition. — Humana Press, 2023. — 537 p. — (Methods in Molecular Biology 2655). — ISBN 978-1-0716-3143-0. This volume explores the latest technologies used in the Polycomb Group of proteins field and helps scientists--working on PcG proteins--investigate all functions of PcG proteins in diverse cellular contexts. The chapters in this book cover topics such the distribution...
2nd edition. — Humana Press, 2019. — 492 p. — (Neuromethods 146) — ISBN: 978-1-4939-9662-9 This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution,...
Humana Press, 2022. — 548 p. — (Methods in Molecular Biology 2530). — ISBN 978-1-0716-2489-0. This volume provides updated protocols for chemical protein synthesis. Chapters guide readers through development methods, strategies, and applications of protein chemical synthesis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter...
2nd edition. — Humana Press, 2022 — 766 p. — (Methods in Molecular Biology 2395). — ISBN 978-1-0716-1816-5. This second edition volume expands on the previous edition with an update on the latest techniques used to study plant systems biology on three specific scales: the molecular level, the tissue level, and the whole plant. Chapters cover topics such as gene regulatory...
Humana Press, 2020. — 891 p. — (Methods in Molecular Biology 2101). — ISBN 978-1-0716-0219-5. This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several...
2nd edition. — Humana Press, 2021. — 484 p. — (Methods in Molecular Biology 2228). — ISBN 978-1-0716-1023-7. This second edition provides new and updated methods on the principles underlying modern protein analysis, from statistical issues to gel-based and mass spectrometry-based applications. Chapters detail protein quantification as basis for realisation of quantitative...
Humana Press, 2022. — 408 p. — (Methods in Molecular Biology 2514). — ISBN 978-1-0716-2403-6. This volume provides detailed protocols for the identification and understanding of vasculogenic mimicry process in vitro and in vivo, in addition to protocols for microscopy and histology. Chapters guide readers through different materials, commercial and homemade scaffolds, Matrigel,...
Humana Press, 2022. — 490 p. — (Methods in Molecular Biology 2435). — ISBN 978-1-0716-2014-4. This volume provides methods and techniques to further the study of cancer immunoprevention. Chapters describe tumor-associated antigens, cancer immune-preventive vaccines, generation of TILs, development of monoclonal antibodies, immunoprofiling technologies, tissue multispectral...
Humana Press, 2019. — 261 p. — (Methods in Molecular Biology, № 2039). — ISBN10: 1493996770, 13 978-1493996773. This volume exploresexperimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the...
Springer Science+Business Media, LLC, 2018. — 297 p. — (Methods in Molecular Biology 1747). — ISBN: 149397694X. This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification. The book also covers methods used to study human/animal and plant nitric oxide. The chapters are divided into three parts: part...
2nd edition. — Humana Press, 2021. — 268 p. — (Methods in Molecular Biology). — ISBN 978-1-0716-1098-5. This completely revised edition explores novel discoveries in bacterial genomic research, with a focus on technical and computational improvements as well as methods used for bacterial pangenome analysis, which relies on microbiome studies and metagenomic data. Beginning with...
Humana Press, 2022. — 640 p. — (Methods in Molecular Biology 2409). — ISBN 978-1-0716-1879-0. This detailed volume explores various aspects of the dengue virus and its four serotypes (DENV1-4). Beginning with a section on protocols to produce DENV and its proteins that are fundamental for many biophysical, biochemical, immunological, and cellular studies, the book continues by...
Humana Press, 2021. — 307 p. — ISBN 978-1-0716-1533-1. This book provides methods and techniques used in construction of global transcriptional regulatory networks in diverse systems, various layers of gene regulation and mathematical as well as computational modeling of transcriptional gene regulation. Written in the highly successful Methods in Molecular Biology series...
Humana Press, 2022. — 459 p. — (Methods in Molecular Biology 2503). — ISBN 978-1-0716-2333-6. This volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the...
3rd edition. — Humana Press, 2021. — 350 p. — (Methods in Molecular Biology, 2305). — ISBN 978-1-0716-1405-1. This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins. Beginning with a section on structural bioinformatics, the book continues with...
Humana Press, 2022. — 861 p. — (Methods in Molecular Biology 2509). — ISBN 978-1-0716-2380-0. This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several...
Humana Press, 2018. — 216 p. — ISBN10: 1493976516, ISBN13: 978-1493976515. This volume provides a collection of methods and protocols detailing extracellular RNA and hopes to be a useful resource for those interested in this exciting and emerging field. Chapters are divided into four sections covering an overview of the field, approaches for the isolation of exRNA, protocols...
Humana Press, 2022. — 479 p. — (Methods in Molecular Biology 2387). — ISBN 978-1-0716-1779-3. This volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen...
Humana Press, 2021. — 1789 p. — (Methods in Molecular Biology 2216). — ISBN 978-1-0716-0978-1. This volume provides readers with an protocol collection and wide-ranging recommendations for preclinical renal MRI used in translational research. The chapters in this book are interdisciplinary in nature and bridge the gaps between physics, physiology, and medicine. They are...
Humana Press, 2020. — 417 p. — (Methods in Molecular Biology 2136). — ISBN: 978-1071604663. This volume details Group A Streptococcus (GAS) research and provides the reader with an extensive collection of research protocols within this important field. Chapters guide readers through standard genetic protocols such as whole genome sequencing, transcriptome analyses, proteome...
Springer, 2018. — 387 p. — (Methods in Molecular Biology 1783). — ISBN: 1493978330. This volume provides experimental and bioinformatics approaches related to different aspects of gene expression analysis. Divided in three sections chapters detail wet-lab protocols, bioinformatics approaches, single-cell gene expression, highly multiplexed amplicon sequencing, multi-omics...
Humana Press, 2019. — 608 p. — (Methods in Molecular Biology 2019) — ISBN: 978-1-4939-9585-1 This volume explores the latest synthetic procedures for producing receptor-specific retinoids and rexinoids, molecular biology methods, and new technologies to demonstrate the therapeutic activities of molecules. The chapters in this book cover topics such as lentiviral delivery of...
Humana Press, 2024. — 239 p. — (Methods in Molecular Biology 2735). — ISBN 978-1-0716-3527-8. This volume covers the latest advances in technologies that look at familial cardiomyopathies in greater detail, and provides new computational and experimental models that model, study, and detect disease at earlier stages. Together, this allows interdisciplinary research experiments...
Humana Press, 2021. — 439 p. — (Methods in Molecular Biology, 2262). — ISBN 978-1-0716-1189-0. This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output. Chapters detail standard methodologies, biochemical methods, Ras processing trafficking and localization,...
Humana, 2024. — 234 p. — ISBN-13 : 978-1071639092 This volume provides references for methods about the proteomics of microbial communities, also called metaproteomics. Chapters guide readers first through specific protein extractions from different environments and/or ecological niches crowded by heterogeneous microbial communities, then deepening the possible complete...
Humana Press, 2022. — 813 p. — (Methods in Molecular Biology 2480). — ISBN 978-1-0716-2241-4. This volume provided methods and protocols on recombinant protein production in different plant systems, downstream processing, and strategies to optimize protein expression. Chapters guide readers through recombinant protein production in important plant systems, protein recovery and...
Humana, 2021. — 359 p. — (Methods in Molecular Biology, 2302). — ISBN 978-1-0716-1393-1. This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to...
Humana Press, 2023. — 1100 p. — (Methods in Molecular Biology 2638). — ISBN 978-1-0716-3024-2. This thorough volume presents a wide range of existing methods, from the very popular to the more exotic, in the area of plant genotyping. Many methods of plant genotyping were initially developed for medical research, but all genotyping methods, if they are to be successful, should...
Humana Press, 2022. — 506 p. — (Methods in Molecular Biology 2396). — ISBN 978-1-0716-1822-6. This volume looks at the latest techniques used by researchers to study various aspects of plant metabolic engineering. The chapters in this book cover topics such as bioinformatics tools used to discover new genes and pathways; heterologous expression of biosynthetic genes in plant...
Humana Press, 2018. — 281 p. — ISBN: 1493986961. —Series: Methods in Molecular Biology, № 1842 This second edition volume expands on the previous edition with updated discussions on new genetic, molecular, and cellular methods used to study somatic stem cells. The chapters in this book focus on the isolation, classification, purity, and plasticity of these stem cells in a...
2nd edition. — Humana Press, 2020. — 673 p. — (Methods in Molecular Biology 2076) — ISBN: 978-1-4939-9882-1. This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the...
Humana Press, 2022. — 972 p. — (Methods in Molecular Biology 2497). — ISBN 978-1-0716-2309-1. This volume details comprehensive protocols and methodologies to assess mitochondrial bioenergetics and dynamics in different tissues and cells involving health and pathological states. Chapters guide readers through methods for assessment of the energy metabolism including Oxygen...
Humana Press, 2015 — 443 p. — (Methods in Molecular Biology 1212) — ISBN: 978-1-4939-2590-2 This volume examines cell-cell interactions and stem cell renewal, two topics that are now inexorably linked as science strives to understand the stem cell niche and its function. Gathering a number of representative protocols, this detailed collection promises to provide readers with...
Humana Press, 2023. — 777 p. — (Methods in Molecular Biology 2639). — ISBN 978-1-0716-3028-0. This volume detials diverse methodological approaches on the assembly and applications of DNA origami assemblies. Chapters guide readers through different synthetic and computational methods, isolation and structural characterization of 2D and 3D DNA origami nanoarchitectures,...
Humana Press, 2020. — 514 p. — (Methods in Molecular Biology 2099). — ISBN 978-1-0716-0211-9. This volume provides various techniques and methodologies currently used in the study of MERS-CoV. Chapters are divided into four parts detailing evolution and entry of MERS-coronavirus, genetic alteration and structural determination of MERS-coronavirus proteins, quantitation of virus...
Humana Press, 2021. — 361 p. — ISBN 978-1-0716-1739-7. This volume details methods of identifying synthetic lethal, genetic interactions by various approaches in different model systems including human cancer cells. Chapters guide readers through genetic interactions in model organisms, RNA interference, CRISPR/Cas9 based genome editing technologies, drug-gene interactions,...
Humana Press, 2018. — 1517 p. — (Methods in Molecular Biology 1776). — ISBN 978-1-4939-7808-3. This volume details protocols on virus-derived nanoparticles (VNPs) for a number of different applications. Chapters guide readers through the production of VNPs derived from plant, animal and bacterial viruses, prokaryotic and eukaryotic expression systems, encapsulation of...
Humana Press, 2025. — 418 р. — ISBN 978-1-0716-4176-7. This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic...
Springer, 2022. — 435 p. — (Methods in Molecular Biology 2377). — ISBN 978-1-0716-1719-9. This book provides state-of-the-art information on gene essentiality screenings in a wide variety of organisms, i.e. screening for protein-coding genes and other genomic elements that are required by an organism to survive under specific conditions. With a focus on the two techniques that...
Humana Press, 2022. — 560 p. — (Methods in Molecular Biology 2375). — ISBN 978-1-0716-1708-3. This volume explores the latest techniques used to study the field of tissue engineered vascular grafts (TEVGs). The chapters in this book cover a wide array of topics such as deriving vascular cells from monocytes and induced pluripotent stem cells; engineering vascular grafts using...
Humana Press, 2024. — 201 p. — (Methods in Molecular Biology 2773). — ISBN 978-1-0716-3714-2. This detailed volume presents a variety of mouse models for cancer studies, from leukemia and lymphoma models to different types of subcutaneous and orthotopic models. Models for the healing process and the assessment of the immune response after local ablative therapies are also...
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