Humana Press, 2009. — 400 p. — ISBN10: 1603271937 Series: Methods in Molecular Biology, №593 This volume discusses the latest developments in clinical proteomics and describes in detail the algorithms used in publicly available software tools. It should be considered as a proteomics-bioinformatics resource and offers the opportunity to understand the details of the various...
Humana Press, 2013. — 225 p. — ISBN: 1603273360, 9781603273367. Series: Methods in Molecular Biology, №972. Microarrays for simultaneous measurement of redundancy of RNA species are used in fundamental biology as well as in medical research. Statistically,a microarray may be considered as an observation of very high dimensionality equal to the number of expression levels...
Springer Science+Business Media, 2016. — 769 p. — (Methods in Molecular Biology 1462). — ISBN: 1493938142. This volume discusses experimental brain injury models that contain valuable information carefully chosen to widen the researchers’ horizon about neurotrauma. Injury Models of Central Nervous System: Methods and Protocols contains relevant experimental design approaches...
Humana Press, 2001. — 252 pp. Series: Methods in Molecular Biology, Volume 168. With the huge increase in available data on the DNA sequences of proteins, there is a growing need to understand and characterize how proteins fold into their biologically active native states and the basis for the stability of these states. In Protein Structure, Stability, and Folding, Kenneth P....
CRC Press LLC, 2003. — 218 р. — ISBN: 1584883278.
Although less than a decade old, the field of microarray data analysis is now thriving and growing at a remarkable pace. Biologists, geneticists, and computer scientists as well as statisticians all need an accessible, systematic treatment of the techniques used for analyzing the vast amounts of data generated by large-scale...
Humana Press, 1995. — 398 p. ISBN: 0896033236 Series: Methods in Molecular Biology, №50 University of York, U.K. Manual of protocols in the identification of many organisms using nucleic acid analysis: plant, insect, and vertebrate DNA; human and animal viruses; bacteria; fungi; protozoans; parasites; and mosquitoes.
Humana Press, 1995. — 457 p. ISBN: 089603321X Series: Methods in Molecular Biology, №49 In Plant Gene Transfer and Expression Protocols, leading experts present the latest step-by-step recipes for introducing genes into model and agricultural plants and for studying gene activity using reporter enzyme assays, RNA techniques, and immunological methods. The book contains not only...
Humana Press, 1995. - 358 p. ISBN: 089603304X Series: Methods in Molecular Biology, №48 Contents Theory and Instrumentation. Electroporation Theory: Concepts and Mechanisms. Effects of Pulse Length and Strength on Electroporation Efficiency. Instrumentation. Electroporation Prools. The Introduction of Proteins into Mammalian Cells by Electroporation. Electroporation of...
MMB vol.047 Humana Press, 1995, ISBN: 0896033104, 369р. Electroporation Protocols for Microorganisms is the first complete guide to the electroporation of nearly all microorganisms of importance used in biological and biomedical research. It includes reproducible protocols for diverse bacterial, fungal, and protist species-many of which are important in human disease-as well as...
Humana Press, 1995. — 278 p. ISBN: 0896032973 Series: Methods in Molecular Biology, №46 Antibiotic Resistance Profiling Bacteriocin Typing Bacteriophage Typing The Analysis of Bacterial Proteins by SDS Polyacrylamide Gel Electrophoresis Lipopolysaccharide Chemotyping Multilocus Enzyme Electrophoresis Fast Atom Bombardment-Mass Spectrometry
Humana Press, 1995, 260 p. ISBN: 0896033082 Series: Methods in Molecular Biology, №45 Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vivo in Preparation for Fusions Yielding Monoclonal Antibodies. Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vitro. Culture Conditions that Optimize Outgrowth of Hybridomas....
Humana Press, 1995. - 412 p. ISBN: B001DA0B6O Series: Methods in Molecular Biology, №44 Agrobacterium Protocols offers beginning and experienced researchers the most comprehensive collection of step-by-step protocols for the genetic manipulation of plants using Agrobacterium. The topics range from the maintenance of bacterial culture collections to aspects of the metabolism and...
Humana Press, 1995. - 312 p. ISBN: 0896032825 Series Methods in Molecular Biology, №43 Alternative Method of Assessing Toxicity. Target Organ Toxicity: LLC-RK1 Cell Screening Test for Nephrotoxicity. Preparation and Use of Cultured Astrocytes for Assay of Gliotoxicity. Human Thyroid Culture. Thyroid Follicular Cells in Monolayer Culture: In Vitro Models for Thyroid Toxicity...
Humana Press, 1995. — 214 p. — (Methods in Molecular Biology 42). — ISBN: 0896032795. Basic Immunology Basic Principles of ELISA Stages in ELISA Theoretical Considerations Direct ELISA Titration of Antigen and Antibody Indirect ELISA Use of Antibodies on Solid-Phase in Capture ELISA Competitive ELISA Immunochemical Techniques
Humana Press, 1995. - 301 p. ISBN: 0896032981 Series: Methods in Molecular Biology, №41 This collection of self-contained, "hands-on" descriptions of established laboratory protocols concentrates on receptor-mediated cell signaling, with particular attention devoted to those receptors that are part of the G-protein-linked superfamily. It offers those already working in the...
Humana Press, 1995. - 368 p. ISBN: 0896033015 Series: Methods in Molecular Biology, №40 Noncovalent Forces Important to the Conformational Stability of Protein Structures. Degradative Covalent Reactions Important to Protein Stability Fluorescence Spectroscopy. Ultraviolet Absorption Spectroscopy. Circular Dichroism. Infrared Spectroscopy. Identifying Sites of Posttranslational...
Humana Press, 1995. - 412 p. ISBN: 0896032728 Series: Methods in Molecular Biology, №39 Baculovirus Expression Protocols offers both industrial and university-based researchers a comprehensive comilation of the latest baculovirus techniques along with step-by-step instructions and time-saving techniques. The contributors-leading authorities in the field-present the assorted...
Humana Press, 1995. - 240 p. ASIN B0019K5V18 Series: Methods in Molecular Biology, №38 Overview Virus Cryopreservation and Storage Freeze-drying and Cryopreservation of Bacteria Freeze-drying of Yeasts Cryopreservation of Yeasts Cultures Cryopreservation and Freeze-drying of Fungi Cryopreservation of Pathogenic and Nonpathogenic Free-Living Amoebae Long-Term Cryopreservation of...
Humana Press, 1994. - 405 p. ISBN: 0896032884 Series: Methods in Molecular Biology, №37 This book provides molecular biology laboratories with the most powerful techniques for employing in vitro transcription and translation systems. Detailed experimental protocols are provided for prokaryotic transcription and translation systems, along with protocols for the many techniques...
Humana Press, 1994. - 310 p. ISBN: 0896032736 Series: Methods in Molecular Biology, №35 Procedures to Improve Difficult Couplings Methods for Removing the Fmoc Group Solvents for Solid-Phase Peptide Synthesis HF Cleavage and Deprotection Procedures for Peptides Synthesized Using a Boc/Bzl Strategy Acid Cleavage/Deprotection in Fmoc/tBu-Solid Phase Peptide Synthesis...
Humana Press, 1995. - 394 p. ASIN B001F7490O Series: Methods in Molecular Biology, №34 Overview of Antibody Use in Immunocytochemistry. Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration. Purification of Antibodies Using Ion-Exchange Chromatography Purification of Antibodies Using Affinity Chromatography Purification of Antibodies Using Protein...
Humana Press, 1994. - 476 p. ISBN: 9780896032804 Series: Methods in Molecular Biology, №33 Preparation of Human Chromosomal Painting Probes from Somatic Cell Hybrids. Reverse Chromosome Painting. FISH Detection on DAPI Banded Chromosomes. Generation of Alphoid DNA Probes for Fluorescence In Situ Hybridization (FISH) Using the Polymerase Chain Reaction. In Situ Hybridization...
MMB vol.032, Humana Press, 26 апр. 1994. - 490р. ISBN: 0896032698 Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the...
Humana Press, 1994. - 388 p. ISBN: 0896032582 Series: Methods in Molecular Biology, №31 Basic Recombinant DNA Techniques:Transformation of Bacteria by Electroporation. Direct Cloning of lgt11 cDNA Inserts into a Plasmid Vector. PCR Cloning Using T- Vectors. Thermal Cycle Dideoxy DNA Sequencing. Ordered Deletions Using Exonuclease III. In Vitro Mutagenesis: Site Directed...
Humana Press, 1994. - 425 p. ISBN: 0896032566 Series: Methods in Molecular Biology, №30 DNase I Footprinting Footprinting with Exonuclease III Hydroxyl Radical Footprinting Hydroxyl Radical Interference 1,10-Phenanthroline-Copper Ion Nuclease Footprinting of DNA-Protein Complexes in Situ Following Mobility-Shift Electrophoresis Assays Identification of Protein-DNA Contacts with...
Humana Press, 1994. - 492 p. ISBN: 0896032892 Series: Methods in Molecular Biology, №29 Mitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution. Chromosome Preparation from Hematological Malignancies. Meiotic Chromosome Preparation. Preparation of Chromosomes for Scanning Electron Microscopy. Immortalized Cell Lines: Chromosome Preparation and...
Humana Press, 1993. - 260 p.ISBN: 9780896032545 Series: Methods in Molecular Biology, №28 About Nonradioactive Nucleic Acid Detection Isolation of DNA from Plants Isolation of High-Molecular-Weight DNA from Animal Cells Restriction Enzyme Digestion, Gel Electrophoresis, and Vacuum Blotting of DNA to Nylon Membranes Isolation of Plant RNA Isolation of Total and PolyA + RNA from...
Humana Press, 1993. — 352 p. ISBN: 0896032507 Series: Methods in Molecular Biology, №27 Crystallization of Membrane Proteins for X-Ray Analysis Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination Biochemical Probes of Topography Antipeptide Antibodies The Production of Monoclonal Antibodies to Membrane Proteins
Humana Press, 1993. — 372 p. — (Methods in Molecular Biology 26). — ISBN: 0896032523. Protecting Groups in Oligonucleotide Synthesis Incorporation of Modified Bases into Oligonucleotides Functionalization of Oligonucleotides with Amino Groups and Attachment of Amino Specific Reporter Groups Functionalization of Oligonucleotides by the Incorporation of Thio-Specific Reporter...
Humana Press, 1993. — 384 p. ISBN: 0896032485 Series: Methods in Molecular Biology, №23 DNA Sequencing Protocols provides you with the knowledge to become a sequencing expert. An all-star cast of investigators covers virtually all aspects, including developments in cycle sequencing, sequencing PCR products, sequencing lambda and cosmids, multiplex sequencing, direct blotting...
Humana Press, 1993. — 274 p. ISBN: 0896032329 Series: Methods in Molecular Biology, №22 This superb survey of the possible applications of physiochemical techniques to the analysis of biological macromolecules is designed for the practicing biochemist or biologist who wants to use the technique but lacks the experience. The contributors emphasize practical aspects, such as...
Humana Press, 1993. — 443 p. ISBN: 0896032396 Series: Methods in Molecular Biology, №21 Culturing and Biological Cloning of Trypanosoma brucei Culturing and Biological Cloning of Trypanosoma cruzi In Vitro Cultivation and Biological Cloning of Leishmania Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages) Suitable for Large-Scale...
Humana Press, 1993. — 496 p. —— (Methods in Molecular Biology 20). — ISBN: 0896032477. Leading researchers summarize and describe their experience and techniques for synthesizing oligonucleotides and their analogs. The protocols presented cover all DNA analogs that have been studied to date and constitute the single most extensive source of knowledge about DNA chemistry available.
Humana Press, 1993. — 354 p. ISBN: 0896032361 Series: Methods in Molecular Biology, №19 Expert practitioners combine the traditional basic techniques of membrane isolation and analysis with modern advances in molecular biology, immunology, and clinical sciences to devise analytical techniques that are successful for expert and novice alike. The techniques are used in the...
MMB vol.018, Humana Press, 1993, ISBN: 0896032450, 436р. Introduction to Transgenesis Transgenic Rodents and the Study of the Central Nervous System Transgenic Animals and the Study of Cancer Transgenic Animals and the Study of the Immune System The Commercial and Agricultural Applications of Animal Transgenesis Commercial and Agricultural Appllcations P Element-Mediated...
Humana Press, 1993. - 380 p. ISBN: 0896032345 Series: Methods in Molecular Biology, №16 Nucleases: An Overview Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1) DNA Polymerases (EC 2.7.7.7) Taq Polymerase (EC 2.7.7.7): With Particular Emphasis on Its Use in PCR Protocols Eukaryotic Nuclear RNA Polymerases (EC 2.7.7.6) Reverse Transcriptase (EC 2.7.7.49): The Use of Cloned...
Humana Press, 1993. - 386 p. ISBN: 0896032442 Series: Methods in Molecular Biology, №15 Polymerase Chain Reaction. Selection of Primers for Polymerase Chain Reaction. Direct Radioactive Labeling of Polymerase Chain Reaction Products. Use of Arithmetic Polymerase Chain Reaction for Synthesis of Single-Stranded Probes for S1 Nuclease Assays. Nonradioactive Labeling of Polymerase...
Humana Press, 1993. - 379 p. ISBN: 0896032264 Series: Methods in Molecular Biology, №14 A General Strategy for Glycoprotein Oligosaccharide Analysis. Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography. Sequence and Linkage Analysis of N- and O-Linked Glycans by Fast Atom Bombardment Mass Spectrometry. Gas Chromatography and Gas...
Humana Press, 1992. - 373 p. ISBN: 0896031993 Series: Methods in Molecular Biology №13 Separation of Large DNA Molecules by Pulsed-Field Gel Electrophoresis. The Isolation of Genomic DNA from Invertebrates. Analysis of Mihondrial DNA Mutations. The Design and Use of Oligonucleotides. The Use of Degenerate Oligonucleotides for Polymerase Chain-Reaction-Based Isolation of Related...
Humana Press, 1991. - 488 p. ISBN: 0896032299 Series: Methods in Molecular Biology Series , №12 Electrophoretic Techniques. Field-Inversion Gel Electrophoresis. Resolving Multi-megabase DNA Molecules Using Contour-Clamped Homogeneous Electric Fields (CHEF). Separation and Size-Determination of DNA over a 10-200 kbp Range. PFGE Using Double-Inhomogeneous Fields or Orthogonal...
Humana Press, 1992. - 338 p. ISBN: 0896032132 Series: Methods in Molecular Biology Series , №11 Thiophilic Adsorption Chromatography. Immobilized Metal Ion Affinity Chromatography. Histidine Ligand Affinity Chromatography. Boronic Acid Matrices for the Affinity Purification of Glycoproteins and Enzymes. Calcium-Dependent Hydrophobic Interaction Chromatography. Lectin Affinity...
MMB vol.010, Humana Press, 1992, 474 р. ISBN: 0896032043 Production of PolyclonaI Antisera Efficient Elution of Purified Proteins from Polyvinylidene Difluoride Membranes (Immobilon) After Transfer from SDS-PAGE and Their Use as Immunogens Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen Synthesis of Pep tides for Use as Immunogens Production and...
Humana Press, 1991. - 458 p. ISBN: 0896032051 Series: Methods in Molecular Biology, №9 Protocols in Human Molecular Genetics highlights the tremendous advances in our ability to work on the human genome that have emerged in the past few years. The latest techniques are set forth in the clear, concise, easy-to-follow format that is the hallmark of Humana's Methods in Molecular...
Humana Press, 1991. - 316 p. ISBN: 089603299X Series: Methods in Molecular Biology, №8 The Retroviral Life Cycle and the Molecular Construction of Retrovirus Vectors Construction of Retroviral Packaging Cell Lines Making High Titer Retroviral Producer Cells Methods of Titrating Nonselectable Recombinant Retroviruses Selectable Eukaryotic-Cell Markers Virus Genome Detection by...
MMB vol.007 Humana Press, 1991, ISBN: 0896031780, 416р. Murray's new handbook on Gene Transfer and Expression Protocols sets forth both current and new methodologies in a clear, concise, easy-to-follow manner, following the successful formula of the classic volumes in Humana's Methods in Molecular Biology series. Each chapter is devoted to a thorough exposition of a single...
Humana Press, 1990. — 557 р. — ISBN: 0685382125 Series: Methods in Molecular Biology, №6 Selection of Media for Tissue and Cell Culture Growth Determination and Medium Analysis Measurements of Viability Suitable for Plant Tissue Cultures Cryopreservation of Plant Cells Hormonal Control of Growth and Development The Initiation and Maintenance of Callus Cultures
Humana Press, 1990. - 704 p. ISBN: 0896031500 Series: Methods in Molecular Biology , №5 Animal Cell Culture, the latest volume in Humana's highly successful Methods in Molecular Biology series, provides detailed practical techniques for the culture of a broad spectrum of basic cell cell types. Chapters offer hands-on methods for creating mammalian fibroblastic cell cultures and...
MMB vol.004, Humana Press, 1988, ISBN: 0896031276 Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde Computer Analysis of DNA Sequences Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique Dligonucleotide Synthesis Using the Manual Phosphotriester Method
Humana Press, 1988. - 524 p. ISBN: 0896031268 Series: Methods in Molecular Biology, №3 Prevention of Unwanted Proteolysis The Bradford Method for Protein Quantitation Amino Acid Analysis by Precolumn Derivatization Identification of N-Terminal Amino Acids by High-Performance Liquid Chromatography Enzymatic Methods for Cleaving Proteins Chemical Cleavage of Proteins Chemical...
Humana Press, 1984. - 366 p. ISBN: 978-0-89603-064-0 (Print) 978-1-59259-489-4 (Online) Series: Methods in Molecular Biology, №2 The Burton Assay for DNA DABA Fluorescence Assay for Submicrogram Amounts of DNA Preparation of RNase-Free DNase by Alkylation The Isolation of Satellite DNA by Density Gradient Centrifugation The Isolation of High Molecular Weight Eukaryotic DNA...
Humana Press, 1984. — 356 р. — ISBN: 0896030628 Series: Methods in Molecular Biology, №01 The most accurate method of determIning protem concentration is probably acid hydrolysis followed by amino acid analysis. Most other methods are sensitive to the amino acid composition of the protein and absolute concentrations cannot be obtained The procedure of Lowry et al. (1) IS no...
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