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Methods in Molecular Biology

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Humana Press, 1993. — 372 p. — (Methods in Molecular Biology 26). — ISBN 0896032523. Protecting Groups in Oligonucleotide Synthesis Incorporation of Modified Bases into Oligonucleotides Functionalization of Oligonucleotides with Amino Groups and Attachment of Amino Specific Reporter Groups Functionalization of Oligonucleotides by the Incorporation of Thio-Specific Reporter Groups...
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Humana Press, 1993. — 496 p. —— (Methods in Molecular Biology 20). — ISBN 0896032477. Leading researchers summarize and describe their experience and techniques for synthesizing oligonucleotides and their analogs. The protocols presented cover all DNA analogs that have been studied to date and constitute the single most extensive source of knowledge about DNA chemistry available.
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Humana Press, 2014. — 313 p. — (Methods in Molecular Biology 1118). — ISBN: 978-1-62703-782-2; 978-1-62703-781-5. Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the...
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Humana Press, 2006. — 744 p. — (Methods in Molecular Biology 332). The complete sequencing of the human genome and many new microarray and proteomic methods for analyzing global changes in both gene expression and protein profiles have greatly increased our ability to understand transmembrane signaling processes in close detail. In this fully updated and expanded second edition of...
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New York: Humana Press, 2015. — 233 p. — (Methods in Molecular Biology 1234). The book is a compilation of a number of conceptual and methodological aspects important for the validation and characterization of intacrine signaling systems. To date, the best-characterized intracrine signaling system is that of angiotensin II (Ang II), covered in depth in various chapters....
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Humana Press, 2013. — 230 p. — (Methods in Molecular Biology 1031). The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In this volume experts in the field explore the design and execution of experiments...
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Humana Press, 2013. — 487 p. — (Methods in Molecular Biology 985). With the ultimate goal of systematically and robustly defining the specific perturbations necessary to alter a cellular phenotype, systems metabolic engineering has the potential to lead to a complete cell model capable of simulating cell and metabolic function as well as predicting phenotypic response to changes...
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Humana Press, 2014. — 360 p. — (Methods in Molecular Biology 1169). The last decade has completely changed our understanding of pathogen recognition in terms of how the innate immune system manages to integrate molecular patterns of completely different pathogens into rather uniform innate immune responses. This immunostimulatory effect of pathogen-derived nucleic acids is of...
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Humana Press, 2012. — 1021 p. — (Methods in Molecular Biology 855). Discovery of genetic material propelled the power of classical evolutionary studies across the diversity of living organisms. Together with early theoretical work in population genetics, the debate on sources of genetic makeup initiated by proponents of the neutral theory made a solid contribution to the...
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Humana Press, 2011. — 288 p. — (Methods in Molecular Biology 718). The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues. In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last few...
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Humana Press, 2014. — 197 p. — (Methods in Molecular Biology 1095). In this volume, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation. These included established methods such as fluorescent and non-fluorescent methods for homogenous assays of Dicer-mediated miRNA maturation or an in vivo assay for Drosha activity....
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3 rd edition. — Humana Press, 2012. — 642 p. — (Methods in Molecular Biology 824). — ISBN 978-1-61779-432-2. This third edition of Recombinant Gene Expression is a lot more than just an update of the previous edition. Although some of the authors that contributed in 2004 were also invited to participate in this new project, this volume contains brand new protocols and topics not...
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Humana Press, 2018. - 394 p. - Volume 1737 of Methods in Molecular Biology. This volume details the most important methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters present methods in sections covering different aspects of the biology of that field: identification of ncRNAs, their differential expression, characterization of their...
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Humana Press, 2002. — 372 p. — (Methods in Molecular Biology 196). Cytochemical Localization of H 2 O 2 in Biological Tissues Localization of Intracellular Lipid Hydroperoxides Using the Tetramethylbenzidine Reaction for Transmission Electron Microscopy The Immunohistochemical Localization of Glutathione Peroxidase 4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody...
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New York: Humana Press, 2015. — 355 p. This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at...
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Humana Press, 2009. — 440 p. — (Methods in Molecular Biology 482). As regenerative medicine involves replacing diseased cells, tissues or organs, or repairing tissues in vivo, the manipulation of stem cells underlie its goals. In this volume leading experts in the field provide an updated representation of the landscape of stem cell-based therapies in a wide spectrum of tissue...
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Humana Press, 2018. - 300 p. - Volume 1693 of Methods in Molecular Biology. This volume details the experimental approaches suitable for isolating and characterizing bacteriophages to formulating bacteriophage medicinal products and clinical application. Chapters guide readers through regulatory compliance and safety aspects of bacteriophage therapy. Written in the highly...
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Humana Press, 2013. — 237 p. — (Methods in Molecular Biology 1023). Small proteins with molecular weights of <25 kDa are involved in major biological processes such as ribosome formation, stress adaption and cell cycle control. The study of the low-molecular-weight proteome has identified many central regulators of biology such as cytokines, chemokines, peptide hormones and...
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Humana Press, 2014. — 268 p. Series: Methods in Molecular Biology, № 1098 The book distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this...
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Humana Press, 2013. — 344 p. Series: Methods in Molecular Biology, № 952 All three peroxisome proliferator-activated receptor (PPAR) subtypes share a high degree of structural homology but differ in function, tissue distribution and ligand specificity. PPARs play critical roles as regulators of numerous physiological as well as pathophysiological pathways, and efforts are...
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Humana Press, 2016. - 225 p. - Volume 1444 of Methods in Molecular Biology. This detailed volume includes a rich variety of applications using various instrumentations, probes, disease models, and targets in order to account for the multidisciplinary nature of the use of in vivo fluorescence imagine. The book also includes chapters on the emerging fields of cell tracking,...
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Humana Press, 2006. — 704 p. Series: Methods in Molecular Biology, № 350 Protein Folding Protocols presents protocols for studying and characterizing protein folding from the unfolded to the folded state. Covering experiment and theory, bioinformatics approaches, and state-of-the-art simulation protocols for better sampling of the conformational space, this volume describes a...
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2 nd ed. — Humana Press, 2004. — 523 P. — E-isbn: 1-59259-774-2. Contents . Preface. Contributors. General issues about recombinant gene expression . Host Cell Compatibility in Protein Expression. Production of Recombinant Proteins: Challenges and Solutions. Folding-Promoting Agents in Recombinant Protein Production. Prokaryotes . Back to Basics: pBR322 and Protein Expression...
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Humana Press, 2017. — 581 p. — ISBN 10 1493968793. — ISBN 13 978-1493968794. Series: Methods in Molecular Biology, № 1584 This volume provides all the essential protocols that are currently used to study the immune synapse. Chapters in The Immune Synapse: Methods and Protocols cover methods for the study of the dynamics of immune synapse assembly, traffic at the immune synapse,...
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Humana Press, 2013. — 233 p. Series: Methods in Molecular Biology, № 995 Many advances in modern neuroscience are enabled by the availability of chemical tools that allow sensitive, precise, and quantitative measurements of, and control over, biological processes. These powerful reagents are widely used for investigating the nervous system at levels of detail ranging from ion...
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Humana Press, USA, 2016. — 211 p.— ISBN: 1493963910 Series: Methods in Molecular Biology, №1481 Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues...
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Humana Press, 2013. — 617 p. — (Methods in Molecular Biology 947). Teratology is the study of chemical-induced birth defects. This book is a comprehensive guide to the procedures and methods commonly employed in the safety testing of all classes of chemical for teratogenicity (also referred to as embryotoxicity, developmental toxicity or prenatal toxicity). The various...
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Humana Press, 2013. — 393 p. — (Methods in Molecular Biology 1001). Tissue engineering and regenerative medicine represents a wide array of cell, biomaterial and cell/biomaterial based approaches focusing on the repair, augmentation, and regeneration of diseases tissues and organs. The book has been assembled in response to the growing interest in organ and tissue regeneration as...
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Humana Press, 2015. — 329 p. Series: Methods in Molecular Biology, № 1245 The ability to produce vast amounts of DNA sequence data has enabled the discovery of molecular markers in model organisms, crops, as well as orphan species making genotyping the rate limiting factor, and this volume focuses on the different markers available and the low to high throughput genotyping of...
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Humana Press, 2010. — 276 p. — (Methods in Molecular Biology 608). — ISBN: 978-1-59745-363-9 (eBook), 978-1-58829-950-5 (Hardcover). Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a...
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Humana Press, 2014. — 239 p. Series: Methods in Molecular Biology, № 1155 Exploring these type II trans-membrane proteins, this volume focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Opening with protocols to understand the signaling process of TNF family members, this detailed volume continues with technical examples of...
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New York: Humana Press, 2015. — 288 p. Although nuclear reprogramming-the cellular process underlying cloning and the artificial generation of stem cells from somatic cells-may be considered a "black box" whose interior events are either completely unknown or at least imperfectly characterized, tantalizing clues about its operation are beginning to emerge. In Nuclear...
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Humana Press, 2018. — 353 p. — (Methods in Molecular Biology 1765). — ISBN: 978-1-4939-7764-2; 978-1-4939-7765-9 This volume explores the latest developments in the study of the mechanisms, diagnostics, screening methods, and therapeutics of colorectal cancer. The book’s chapters are divided into three parts: the chapters in Part One examine techniques used to study the molecular...
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Humana Press, 2013. — 373 p. Series: Methods in Molecular Biology, № 988 Glyco-engineering is being developed as a method to control the composition of carbohydrates and to enhance the pharmacological properties of monoclonal antibodies (mAbs) and other proteins. In this volume experts in the field provide readers with production and characterization protocols of glycoproteins and...
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Humana Press, 2013. — 235 p. — (Methods in Molecular Biology № 975). Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant’s development and its interactions with the biotic or abiotic environment. Functional genomics is vastly lagging behind the speed of genome sequencing...
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Humana Press, 2008. — 536 p. Series: Methods in Molecular Biology, № 395 The book provides a collection of robust protocols for molecular biologists beginning to use comparative genomic analysis tools in a variety of areas. Volume I contains the first four of seven sections. In the first section, the reader is introduced to genomes via a number of visualization tools that...
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Humana Press, 2007. — 426 p. Series: Methods in Molecular Biology, № 396 The book provides a collection of robust protocols for molecular biologists beginning to use comparative genomic analysis tools in a variety of areas. Volume 2 contains the last three of seven sections. In the second volume, the fifth section describes a number of tools for comparative analysis of domain...
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Humana Press, 2014 — 402p. — (Methods in Molecular Biology 1115) — ISBN: 978-1-62703-767-9 (eBook), 978-1-62703-766-2 (Hardcover). Plant taxonomy is an ancient discipline facing new challenges with the current availability of a vast array of molecular approaches which allow reliable genealogy-based classifications. Although the primary focus of plant taxonomy is on the...
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Humana Press, 2014. — 245 p. Series: Methods in Molecular Biology, № 1160 The book focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an...
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Humana Press, 2013. — 395 p. — ISBN 978-1-62703-283-4. Series: Methods in Molecular Biology, № 977 The goal of the book is to provide scientists in academia, food, and pharmaceutical industry, as well as public institutions, complementary technologies for investigating various facets of regulatory systems that contribute to the control of protein- coding genes in mammalian...
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Humana Press, 2014 — 2nd Ed. — 306p. — (Methods in Molecular Biology 1127) — ISBN: 978-1-62703-986-4 (eBook), 978-1-62703-985-7 (Hardcover). Plant-Pathogen Interactions: Methods and Protocols, Second Edition expands upon the first edition with current, detailed protocols for the study of plant pathogen genome sequences. It contains new chapters on techniques to help identify...
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Springer Science+Business Media LLC., 2017. — 265 p. — (Methods in Molecular Biology 1616) — ISBN 1493970356. This volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike. Chapters detail common bacterial pathogens, protocols that can be...
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Humana Press, 2018. — 382 p. — ISBN 978-1-4939-7456-6 Series: Methods in Molecular Biology, № 1702 There are many definitions striving to capture the hidden meaning of “Systems Biology.” Indeed, despite many efforts, the concept of Systems Biology remains quite uncertain. As already highlighted, currently two primary streams can be recognized within Systems Biology: (1) pragmatic...
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Humana Press, 2015. — 339 p. — (Methods in Molecular Biology 1309). This volume provides the plant scientific community with a collection of established and recently developed experimental protocols to study plant gravitropism. The first few chapters in this book discuss topics such as methods to properly orient plant material for gravitropism studies; protocols for data...
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Humana Press, 2018. - 351 p. - Volume 1722 of Methods in Molecular Biology/ This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses...
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Humana Press, 2016 — 165p. — (Methods in Molecular Biology 1393) — ISBN: 978-1-4939-3338-9 (eBook), 978-1-4939-3336-5 (Hardcover). This volume explores the still undiscovered secrets of tumor immunology and cancer immunology by discussing the methods and techniques that world-renowned experts in the field use in their laboratories. This book provides a better understanding of...
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Humana Press, 2018. — 373 p. — (Methods in Molecular Biology 1715). This detailed volume describes a spectrum of methods and protocols that can be used for the bench-to-bedside development and evaluation of retinal gene therapy. Methods for the successful delivery of these gene therapy vector systems to the retina are examined, as well as assays to test the efficacy in vitro in...
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Humana Press, 2018. - 347 p. - Volume 1685 of Methods in Molecular Biology. This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful...
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Humana Press, 2016. — 186 p. — (Methods in Molecular Biology 1373). This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using...
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Humana Press, 2016. — 311 p. This volume provides simple and accessible experiment protocols to explore thymus biology. The book is divided into three parts presenting short reviews on T cell development, analysis strategies, protocols for cell preparation, flow cytometry analyses, and multiple aspects of thymocyte biology. As a volume in the highly successful Methods in Molecular...
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Humana Press, 2017. — 357 p. — (Methods in Molecular Biology 1519). This volume details experimental approaches used to investigate phagocytosis and phagosome maturation. Chapters present methods and protocols on quantifying uptake and phagosome maturation using biophysical and biochemical approaches, proteomics, microscopy, and flow cytometry. Written in the highly successful...
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New York: Humana Press, 2015. — 485 p. This volume provides readers with current methods to study RNA remodeling proteins. The methods, ranging from basic to complex, help the scientific community understand the role and fate of RNA species in cells, and their structures and interactions with other biomolecules. The book begins with two introductory chapters, followed by chapters...
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Humana Press, 2014. — 279 p. Series: Methods in Molecular Biology, № 1133 The book is a collection of laboratory protocols covering current methods that are employed to measure and detect activities of these proteases in diverse biological systems, ranging from unicellular organisms to mammals. Broken into two parts, the first part focuses on methods to measure, detect, and...
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Humana Press, 2011. - 523 p. - Volume 736 of Methods in Molecular Biology. - With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Atomic Force Microscopy in Biomedical Research: Methods...
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Humana Press, 2012. — 614 p. — (Methods in Molecular Biology 845). Microbiologists, medical mycologists, immunologists, and biochemists are increasingly working together to focus on the processes involved in the progression and treatment of fungal disease. This volume is designed for research scientists who are involved in this work and interested in undertaking new or comparative...
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Humana Press, 2013. — 445 p. — (Methods in Molecular Biology 1022). Glycosyltransferases (GTs) are essential for the biosynthesis of complex glycoconjugates and are powerful tools to study the functions of complex glycans in health, development and disease. Complex glycoconjugates, such as glycoproteins, proteoglycans and glycolipids, are assembled by GTs which synthesize specific...
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Humana Press, 2006. - 401 p. Series: Methods in Molecular Biology, №347 Glycobiology involves studies of complex carbohydrates and posttrans- tional modifications of proteins, and has become an important interdiscip- nary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Although initial research was directed toward elucidation of the different...
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Humana Press, 2016 — 228p. — (Methods in Molecular Biology 1396) — ISBN: 978-1-4939-3344-0 (eBook), 978-1-4939-3342-6 (Hardcover). This volume combines protocols that encompass the true variety of investigation done on superantigens in the fields of microbiology, immunology, molecular biology, biochemistry, and cellular biology, with a strong focus on disease models utilized to...
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Humana Press, 2016 — 544p. — (Methods in Molecular Biology 1377) — ISBN: 978-1-4939-3179-8 (eBook), 978-1-4939-3178-1 (Hardcover). This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases. P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all...
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Humana Press, 2013. — 630 p. — (Methods in Molecular Biology 954). Legionnaires’ disease is a severe form of pneumonia that can occur in epidemics of several hundred cases. This atypical pneumonia is characterized by a high mortality rate and affects primarily elderly and immuno-compromised individuals. An in-depth understanding of the ecology and virulence of Legionella spp. will...
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Humana Press, 2015 — 261p. — (Methods in Molecular Biology 1310) — ISBN: 978-1-4939-2690-9 (eBook), 978-1-4939-2689-3 (Hardcover). This volume aims to bring together a variety of protocols useful for DNA-based typing of blood cell antigens. Protocols range from simple approaches with low technical complexity to highly sophisticated modern developments. Written for the Methods...
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Humana Press, 1991. - 488 p. ISBN 0896032299 Series: Methods in Molecular Biology Series , №12 Contents Electrophoretic Techniques. Field-Inversion Gel Electrophoresis. Resolving Multi-megabase DNA Molecules Using Contour-Clamped Homogeneous Electric Fields (CHEF). Separation and Size-Determination of DNA over a 10-200 kbp Range. PFGE Using Double-Inhomogeneous Fields...
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Humana Press, 1993. - 380 p. ISBN 0896032345 Series: Methods in Molecular Biology, №16 Contents Nucleases: An Overview Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1) DNA Polymerases (EC 2.7.7.7) Taq Polymerase (EC 2.7.7.7): With Particular Emphasis on Its Use in PCR Protocols Eukaryotic Nuclear RNA Polymerases (EC 2.7.7.6) Reverse Transcriptase (EC 2.7.7.49):...
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Humana Press, 2001. — 452 p. — (Methods in Molecular Biology 43). With the molecular mechanisms underlying the various forms of muscular dystrophy now rapidly clarifying, precise diagnosis has become a reality, and even a requirement in clinical practice. In Muscular Dystrophy: Methods and Protocols, Katherine Bushby and Louise Anderson have assembled an outstanding collection of...
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Humana Press, 2016 — 243p. — (Methods in Molecular Biology 1370) — ISBN: 978-1-4939-3142-2 (eBook), 978-1-4939-3141-5 (Hardcover). This volume aims to present a large panel of techniques for the study of Plant Cell Division. Plant Cell Division: Methods and Protocols captures basic experimental protocols that are commonly used to study plant cell division processes, as well...
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Humana Press, 2016. — 240 p. — (Methods in Molecular Biology 1356). This volume provides detailed discussion of a variety of important techniques that researchers use to study fungal molecular biology and pathogenesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents,...
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Humana Press, 2018. — 447 p. — (Methods in Molecular Biology 1709). This volume is a compilation of laboratory protocols and methodology required for the study of molecular chaperones and the cellular stress response. Chapters detail stress response in Hsf1, Hsf2 and Hsf4 knockout mice, mapping HSP interaction networks, the LUminescence-based Mammalian IntERactome (LUMIER), Hsp70...
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Humana Press, 2014. — 289 p. Series: Methods in Molecular Biology, № 1148 The book focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of...
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Humana Press, 2016 — 332p. — (Methods in Molecular Biology 1406) — ISBN: 978-1-4939-3444-7 (eBook), 978-1-4939-3442-3 (Hardcover). This volume provides resources, ideas, and bench manuals for the study of breast cancer. This book is divided into five sections: methods used in clinical laboratory for diagnosis (Detection of Molecular Markers of Breast Cancer); methods used in...
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Humana Press, 2013. — 243 p. Series: Methods in Molecular Biology, № 1013 Chemokines constitute a large family of structurally similar cytokines that contain a signature of conserved cysteine residues joined by disulfide bridges. Binding of chemokines to specific G protein-coupled receptors followed by downstream signaling defines their biological function. Initially,...
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New York: Humana Press, 2015. — 186 p. The book offers a collection of methods for those interested in the discovery, localization, and functional analysis of these non-coding transcripts that have the potential and ability to orchestrate and control gene expression. After a review of the field, this detailed volume continues with methods useful for the study of siRNAs, microRNAs...
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Humana Press, 2005. — 352 p. — (Methods in Molecular Biology 309). — ISBN 1-59259-935-4. Identification of Components of RNAi Pathways Using the Tandem Affinity Purification Method Separation of Drosophila RNA Silencing Complexes by Native Gel Electrophoresis Preparation and Analysis of Drosha RNA Interference in Caenorhabditis elegans Down-Regulating Gene Expression by RNA...
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Humana Press, 2004. — 326 p. Series: Methods in Molecular Biology, № 274 A comprehenive and cutting-edge collection of basic and advanced biochemical techniques for isolating, treating, and analyzing the photosynthetic materials and processes. All protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory...
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Humana Press, 2013. — 554 p. Series: Methods in Molecular Biology, № 927 Semen analysis methods. Tests of sperm function. Sperm DNA damage testing. Advanced clinical testing. Sperm preparation and selection techniques. Staging and histology techniques. Oxidative stress testing. Analytical tools and methods. Methodologies to evaluate sperm epigenetics. In vitro and ex...
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Humana Press, 2014 — 347p. — (Methods in Molecular Biology 1128) — ISBN: 978-1-62703-974-1 (eBook), 978-1-62703-973-4 (Hardcover). In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory....
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Humana Press, 2010.— 407 p. Series: Methods in Molecular Biology, ISBN: 978-1603272407, e-ISBN: 978-1603272414. Whereas getting exact data about living systems and sophisticated experimental procedures have primarily absorbed the minds of researchers previously, the development of high-throughput technologies has caused the weight to increasingly shift to the problem of...
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Humana Press, 2017. — 221 p. — (Methods in Molecular Biology 1582). While HIV-1 continues to be well-researched, this detailed volume draws attention to other members of the Retrovirus family, namely the Human T-lymphotropic Viruses (HTLVs), featuring the most updated technical information about HTLV determination and the methods to investigate their interaction with the host...
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Humana Press, 2015 — 439p. — (Methods in Molecular Biology 1321) — ISBN: 978-1-4939-2760-9 (eBook), 978-1-4939-2759-3 (Hardcover). Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of...
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Humana Press, 2012. — 269 p. — (Methods in Molecular Biology 825). The germline is unique in mammals as it is the only cell lineage that undergoes mitosis, meiosis, and differentiation, making these cells amenable to in-depth genomic and mechanistic studies. In this volume expert researchers provide a wide-ranging collection of techniques that can be applied to the study of both...
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Humana press, 1996. — 344 p. Series: Methods in Molecular Biology, № 61 The purpose of the preface is to explain the book's objectives and how to use it; give warnings, disclaimers, and the like.* The main objective of Protein and Peptide Analysis by Mass Spec trometry is quite straightforward—to present authoritative, up-to-date, and practical accounts of the use of mass...
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Humana Press, 2014. — 247 p. — ISBN: 1627036784 Series: Methods in Molecular Biology, №1089 Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics...
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Humana Press, 1994. - 476 p. ISBN 9780896032804 Series: Methods in Molecular Biology, №33 Contents Preparation of Human Chromosomal Painting Probes from Somatic Cell Hybrids. Reverse Chromosome Painting. FISH Detection on DAPI Banded Chromosomes. Generation of Alphoid DNA Probes for Fluorescence In Situ Hybridization (FISH) Using the Polymerase Chain Reaction. In...
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Humana Press, 2012. - 690 p. ISBN: 161779841X Series: Methods in Molecular Biology, №882 The HLA molecules are important regulators of the immune response through mediating antigen presentation and interaction between key immune mediating cells. They are also the major histocompatibility barriers to transplantation, which is the clinical paradigm of the self versus non self...
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Humana Press, 1995. — 398 p. ISBN 0896033236 Series: Methods in Molecular Biology, №50 University of York, U.K. Manual of protocols in the identification of many organisms using nucleic acid analysis: plant, insect, and vertebrate DNA; human and animal viruses; bacteria; fungi; protozoans; parasites; and mosquitoes.
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Humana Press, 2010. — 2nd Ed. — 472 p. — (Methods in Molecular Biology 622). — ISBN: 978-1-60327-299-5 (eBook), 978-1-60327-298-8 (Hardcover). Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse...
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Humana Press, 2012. - 250 p. - Methods in Molecular Biology, v 818. - SELDI is distinct from other TOF-MS technologies in that it couples features of chromatography and mass spectrometry, facilitating analyte enrichment and sample cleanup on an array surface. In the growing field of proteomics, SELDI technology has been widely used for biomarker discovery and characterization in...
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Humana Press, 2017. — 461 p. — (Methods in Molecular Biology 1559). This volume presents a broad selection of cutting-edge methods and tools that will enable the reader to investigate the multi-faceted manifestations of inflammation. Inflammation: Methods and Protocols is divided into four sections: the first three sections describe protocols investigating immune-mediated...
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Humana Press, 1991. - 316 p. ISBN 089603299X Series: Methods in Molecular Biology, №8 The Retroviral Life Cycle and the Molecular Construction of Retrovirus Vectors Construction of Retroviral Packaging Cell Lines Making High Titer Retroviral Producer Cells Methods of Titrating Nonselectable Recombinant Retroviruses Selectable Eukaryotic-Cell Markers Virus Genome...
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Humana Press, 1991. - 458 p. ISBN 0896032051 Series: Methods in Molecular Biology, №9 Protocols in Human Molecular Genetics highlights the tremendous advances in our ability to work on the human genome that have emerged in the past few years. The latest techniques are set forth in the clear, concise, easy-to-follow format that is the hallmark of Humana's Methods in Molecular...
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Humana Press, 1999. - 412 p. Series: Methods in Molecular Biology, №121 Contents Cloning Human Natural Killer Cells Generation of Short-Term Murine Natural Killer Cell Clones to Analyze Ly49 Gene Expression Cloning and Culturing of Fetal Mouse Natural Killer Cells Techniques for Studying Development of Human Natural Killer Cells and T Cells Murine Natural Killer Cell...
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Humana Press, 2016. - 309 p. - ISBN 978-1-4939-3668-7 Series: Methods in Molecular Biology, №1435 Protocols for West Nile Virus Research is a collection of research methods, techniques, and approaches to investigate the molecular biology of West Nile virus (WNV). The target audience is comprised of researchers such as virologists, molecular biologists, microbiologists, and other...
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Humana Press, 2017. — 375 p. — (Methods in Molecular Biology 1550). This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the...
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Humana Press, 2014. — 317 p. Series: Methods in Molecular Biology, № 1153 The book is a collection of detailed techniques that will be a useful tool for a wide range of plant biologists, as well as for scientists of other fields interested in plant isoprenoids. Isoprenoids are an incredibly diverse family and they participate in a large variety of processes. Divided into four...
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Humana Press, 2012. - 278 p. ISBN: 162703112X The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In Recombinant and In Vitro RNA Synthesis: Methods and Protocols, expert researchers in the field detail approaches from the...
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Humana Press, 2000. — 418 p. Series: Methods in Molecular Biology, № 125 Anthony P. Corfield and a group of hands-on expermentalists bring together in this volume a state-of-the-art collection of reliable and tested methods for the study of all aspects of mucins. These powerful methods range from the preparation of mucins to their detection and quantitation with molecular...
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Humana Press, 2007. — 240 p. Series: Methods in Molecular Biology, № 370 The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays...
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New York: Humana Press, 2016. - 349 p. Series: Methods in Molecular Biology, № 1342 This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest...
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Humana Press, 2018. - 356 p. - Volume 1770 of Methods in Molecular Biology. This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme...
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Humana Press, 1997. — 332 p. Series: Methods in Molecular Biology, № 69 The first libraries of complementary DNA (cDNA) clones were con­ structed in the mid-to-late 1970s using RNA-dependent DNA polymerase (reverse transcriptase) to convert poly A* mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors. Since then cDNA technology has become a fundamental...
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Humana Press, 2012. — 416 p. — (Methods in Molecular Biology 854). Protein analysis is increasingly becoming a cornerstone in deciphering the molecular mechanisms of life. Proteomics, the large-scale and high-sensitivity analysis of proteins, is already pivotal to the new life sciences such as Systems Biology and Systems Medicine. Proteomics, however, relies heavily on the past...
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Humana Press, 2013. — 262 p. Series: Methods in Molecular Biology, № 1003 Despite the best efforts of many and despite landmark discoveries and experimental ingenuity, challenges in the pursuit of research related to olfactory receptors (ORs) continue to exist. In this volume, experts in the field contribute chapters that serve to address these challenges. The volume consists of...
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Humana Press, 2007. — 260 p. Series: Methods in Molecular Biology, № 389 The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on...
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Humana Press, 2016. — 298 p. Series: Methods in Molecular Biology №1352. This expert volume provides insights into the technological fundamentals together with a comprehensive overview of the potentialities of peptide microarray technology in basic research and clinical assays. Advancements made in recent years in peptide library synthesis, immobilization chemistry and array...
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Humana Press, 1995. - 214 p. ISBN 0896032795 Series: Methods in Molecular Biology, №42 Contents Basic Immunology Basic Principles of ELISA Stages in ELISA Theoretical Considerations Direct ELISA Titration of Antigen and Antibody Indirect ELISA Use of Antibodies on Solid-Phase in Capture ELISA Competitive ELISA Immunochemical Techniques
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Second Edition. — Humana Press, 2009. — 574 p. Series: Methods in Molecular Biology, №516 The Enzyme Linked Immunosorbent Assay (ELISA) continues, as predicted, to be the predominant assay used in biomedical and agricultural research. While little has changed in the principles of the ELISAs used since the publication of The ELISA Guidebook, many technological advantages,...
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Humana Press, 1995. — 223 p. — ISBN 0-89603-279-5. ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also...
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Humana Press, 2010. — 290 p. Series: Methods in Molecular Biology, № 599. Since its first systematic application during the 1970s, bioremediation, or the exploitation of a biological system’s degradative potential to combat toxic pollutants such as heavy metals, polyaromatic hydrocarbons (PAH), cyanides, and radioactive material, has proven itself over time, and the many advances...
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Humana Press, 2011 — 473p. — (Methods in Molecular Biology 677) — ISBN: 978-1-60761-869-0 (eBook), 978-1-60761-868-3 (Hardcover). Over the past several years, a high diversity of regulatory cells and suppressive molecules has taken centre stage in the field of immunoregulation.In Suppression and Regulation of Immune Responses: Methods and Protocols, expert researchers highlight...
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Humana Press, 2016 — 260p. — (Methods in Molecular Biology 1371) — ISBN: 978-1-4939-3139-2 (eBook), 978-1-4939-3138-5 (Hardcover). This second volume expands upon the previous edition with new research and objectives in immunoregulation and immune tolerance. Chapters cover topics ranging from new molecular and cellular mechanisms of tolerance; generation and characterization of...
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Humana Press, 2003. — 269 p. Series: Methods in Molecular Biology, № 239 Cell migration is now well recognized as a critical component of the inflammatory disease process, so that its proper understanding promises to generate both ground-breaking basic discoveries and the development of novel therapeutics. In this volume leading cell biologists and immunologists present their most...
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Humana Press, 2008. — 438 p. — (Methods in Molecular Biology). Drosophila is a comprehensive collection of methods and protocols for Drosophila, one of the oldest and most commonly used model organisms in modern biology. The protocols are written by the scientists who invented the methods. The text presents a diverse set of techniques that range from the basic handling of flies...
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Humana Press, 2017. — 305 p. — ISBN 1493968645. Series: Methods in Molecular Biology, N 1580 This volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ hybridization. The chapters in MicroRNA...
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Humana Press, 2010. — 324 p. — (Methods in Molecular Biology, vol.626). — ISBN-10: 1607615843, ISBN-13: 978-1607615842. Product Description Spanning from discoveries in fundamental immunology to industrial and commercial concerns, the study of vaccine adjuvants has developed into an exciting area of work with great, vital potential in innovating techniques in which...
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Humana Press, 1995, 260 p. ISBN 0896033082 Series: Methods in Molecular Biology, №45 Contents Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vivo in Preparation for Fusions Yielding Monoclonal Antibodies. Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vitro. Culture Conditions that Optimize Outgrowth of...
  • №114
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Humana Press, 1995. - 240 p. ASIN B0019K5V18 Series: Methods in Molecular Biology, №38 Contents Overview Virus Cryopreservation and Storage Freeze-drying and Cryopreservation of Bacteria Freeze-drying of Yeasts Cryopreservation of Yeasts Cultures Cryopreservation and Freeze-drying of Fungi Cryopreservation of Pathogenic and Nonpathogenic Free-Living Amoebae Long-Term...
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2nd Edition. - Humana Press, 2007. - 362 p. - Volume 368 of Methods in Molecular Biology. - Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not...
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Humana Press Inc., Totowa, NJ. - 2003. -248 p. Methods in Molecular Biology, vol. 249 A collection of biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants,...
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Humana Press, 2017. — 257 p. — (Methods in Molecular Biology 1544). This volume provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue. Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem...
  • №118
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Humana Press, 2010. - 287 p. Methods in Molecular Biology, Vol 627 While commercial instruments have expanded the usage and the related literature has increased, the quality of surface plasmon resonance (SPR) research has been hindered by a lack of knowledge of the processes that influence the SPR signal. In Surface Plasmon Resonance: Methods and Protocols, experts in the...
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Humana Press, 2013. — 228 p. Series: Methods in Molecular Biology, № 1040 Inflammasomes have a seemingly simple architecture. A sensor molecule of the NLR or PYHIN protein family recruits the common adapter molecule apoptosis-associated speck-like protein containing a CARD (ASC). Even so, inflammasome activation is highly regulated at multiple steps and precise details of how...
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2nd Edition. — Humana Press, 2014. — 607 p. Series: Methods in Molecular Biology, №1184 At the intersection of experimental and computational sciences, the second edition of Immunoinformatics provides biological insights as well as a simpler way to implement approaches and algorithms in the immunoinformatics research domain. After an introductory section, this extensive...
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Humana Press, 2013. — 373 p. Series: Methods in Molecular Biology, № 959 Organogenesis entails the regulation of cell division, cell expansion, cell and tissue type differentiation, and patterning of the organ as a whole. It is essential to gain insight into how organs are initiated and how they develop. However, this very often is subject to technical difficulties as these...
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2nd Edition. — Humana Press, 2013. — 238 p. — ISBN 978-1-62703-235-3 Series: Methods in Molecular Biology, № 962 We are proud to present the second edition of p53 protocols to keep up with the progress in p53 research since the publication of its fi rst edition. The present edition includes 19 chapters delineating state-of-the art methods to address the challenging questions of...
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Humana Press, 2015 — 275p. — (Methods in Molecular Biology 1293) — ISBN: 978-1-4939-2519-3 (eBook), 978-1-4939-2518-6 (Hardcover). The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and...
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Humana Press, 2013. — 402 p. Series: Methods in Molecular Biology, № 966 In recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere. In this volume,...
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Springer, 2012. — 292 p. 77 illus., 18 illus. in color. — ISBN: 1627031065, 9781627031066 Series: Methods in Molecular Biology, №939 The post-genomic revolution is witnessing the generation of petabytes of data annually, with deep implications ranging across evolutionary theory, developmental biology, agriculture, and disease processes. Data Mining for Systems Biology:...
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Humana Press, 2015. — 278 р. — ISBN-10: 1493920197, ISBN-13: 978-1493920198 Methods in Molecular Biology, №1248 This volume provides an overview of modern and emerging methods for production, analysis, and utility of peptide libraries. Chapter focus on methods and techniques for synthesis, genetic expression, hybrid synthesis-expression, examples of modern utility of these...
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Humana Press, 2013. — 460 p. Series: Methods in Molecular Biology, № 1044 Genetic toxicology is recognized by geneticists and researchers concerned with the genetic impact of man-made chemicals. In this volume expert researchers in the field provide comprehensive genetic toxicology protocols. These include in vitro and in vivo protocols on mutation assays, cytogenetic techniques,...
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Humana Press, 2017. - 320 p. Volume 1553 of Methods in Molecular Biology. This volume contains a collection of protocols from some of the major laboratories involved in stem cell research across the world. The research discussed in this book covers topics such as: isolating, characterizing and expanding dental pulp stem cells; manipulating the proliferative potential of...
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Humana Press, 2012. - 419 p. ISBN: 1627030883 Series: Methods in Molecular Biology, №938 Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply. Phytoplasmas can cause serious and devastating problems for crop...
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Humana Press, 2011. - 328 p. - Methods in Molecular Biology, Vol. 682. - Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore...
  • №131
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Humana Press, 2006. — 389 p. Series: Methods in Molecular Biology, № 335 Although fluorescent probes and assays, which use energy transfer (ET) for monitoring DNA reactions, have multiplied in recent years, until now there have been no manuals summarizing the many different protocols and probe designs. Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is the...
  • №132
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Humana Press, 2002. - 279 p. - Methods in Molecular Biology, Vol. 203. - Detection and analysis of DNA damage is critically important in the study of the widest variety of biological phenomena, ranging from apoptosis and aging to heart diseases and anticancer drug development. In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts...
  • №133
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Humana Press, 2017. — 213 p. — (Methods in Molecular Biology 1644). — ISBN 10 1493971859, ISBN 13 978-1493971855. This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the "research...
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Humana Press, 2018. — 223 p. — (Methods in Molecular Biology 1724). This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico. Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters...
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Humana Press, 2014. — 366 p. Series: Methods in Molecular Biology, № 1090 In this volume, expert researchers in the field provide detailed experimental procedures for each step of the flux quantification workflow. Steady state and dynamic modeling are considered, as well as recent developments for the reconstruction of metabolic networks and for a predictive modeling. Written in...
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Humana Press, 2011. — 434 p. — (Methods in Molecular Biology 675). International biobank collaborations allow for studies with large number of subjects where generalizability of findings across populations can be investigated, which means establishing quality criteria concerning the nature of the sample, conditions of sample storage, and the adequacy of available information is of...
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Springer Science+Business Media LLC, 2017. — 283 p. — (Methods in Molecular Biology 1620) — ISBN-10: 1493970593. This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field. Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food...
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Humana Press, 2014. — 395 p. Series: Methods in Molecular Biology, № 1147 The discovery that most of the chronic infections in humans, including the oral, lung, vaginal and foreign body-associated infections, are biofilm-based, has prompted the need to design new and properly focused preventive and therapeutic strategies for these diseases. Microbial Biofilms: Methods and...
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Humana Press, 2012. — 419 p. — (Methods in Molecular Biology 805). Display technologies have become a very powerful way of generating therapeutic lead molecules and specific reagents for increasing our understanding of biology; however, despite being first described shortly after phage display, the use of ribosome display and related methods have been much less widespread. Since...
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2 nd ed. — Humana Press, 2004. — 500 p. — e-ISBN: 1-59259-809-9 Series: Methods in Molecular Biology, № 278 Contents Screening and Optimizing Protein Production in E. coli Isotopic Labeling of Recombinant Proteins From the Methylotrophic Yeast Pichia pastoris Perdeuteration/Site-Specific Protonation Approaches for High-Molecular-Weight Proteins Segmental Isotopic Labeling for...
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Humana Press, 2012. - 326 p. Series: Methods in Molecular Biology, №803 The multidisciplinary science of chemical proteomics studies how small molecules of synthetic or natural origin bind to proteins and modulate their function. In Chemical Proteomics: Methods and Protocols, expert researchers in the field provide key techniques to investigate chemical proteomics focusing on...
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Humana Press, 2017. — 289 p. — (Methods in Molecular Biology 1522). This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and...
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Humana Press, 2009. — 302 p. Series: Methods in Molecular Biology, № 529 DNA microarray technology has revolutionized research in the past decade. Initially an application for mRNA expression studies, the technology now has spread to other applications such as comparative genomic hybridization, SNP and mutation analysis. In this volume experts explore these now commonly used...
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New York: Humana Press, 2012. — 492 p. — (Methods in Molecular Biology 863). — ISBN 978-1-61779-611-1, 978-1-61779-612-8. Includes epigenetic alterations that can be use in clinical practice Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts The epigenetic regulation plays an important role in normal...
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Humana Press, 1994. - 320 p. ISBN 0896032744 Series: Methods in Molecular Biology, №36 Contents Gel-Filtration Chromatography Ion-Exchange Chromatography Reversed-Phase HPLC: Analytical Procedure Reversed-Phase High-Performance Liquid Chromatography: A Semipreparative Methodology Applications of Strong Cation-Exchange (SCX)-HPLC in Synthetic Peptide Analysis Principles...
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Humana Press, 1994. - 310 p. ISBN 0896032736 Series: Methods in Molecular Biology, №35 Contents Procedures to Improve Difficult Couplings Methods for Removing the Fmoc Group Solvents for Solid-Phase Peptide Synthesis HF Cleavage and Deprotection Procedures for Peptides Synthesized Using a Boc/Bzl Strategy Acid Cleavage/Deprotection in Fmoc/tBu-Solid Phase Peptide...
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Humana Press, 2012. — 497p. — (Methods in Molecular Biology 847). — ISBN 978-1-61779-557-2 In 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture. In Transgenic Plants: Methods and Protocols, Second Edition expert researchers in the field provide...
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Humana Press, 2016 — 387p. — (Methods in Molecular Biology 1398) — ISBN: 978-1-4939-3356-3 (eBook), 978-1-4939-3354-9 (Hardcover). This volume describes different up-to-date methodological approaches, ranging from physiological assays to imaging and molecular techniques, to study a wide variety of plant responses to environmental cues. Environmental Responses in Plants:...
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Springer, 2019. — 259 p. — (Methods in Molecular Biology 1906). — ISBN 1493989634. This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample...
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Humana Press, 2014 — 284p. — (Methods in Molecular Biology 1123) — ISBN: 978-1-62703-968-0 (eBook), 978-1-62703-967-3 (Hardcover). Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage...
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New York: Humana Press, 2015. — 477 p. This volume is essential for geneticists, molecular biologists, biochemists, and medical doctors interested in the use of mouse models in cancer research. Recent genome studies, together with refined genetic engineering techniques, have greatly increased the value of using mice for research on cancer and other human disorders. The chapters of...
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Humana Press, 2014. — 283 p. Series: Methods in Molecular Biology, № 1134 The book describes a number of genetic, biochemical and immunological techniques. These techniques provide an advancing understanding of the pathology, breakdown of the immune system and therapeutic challenges of SLE in both humans and animal models. Written in the highly successful Methods in Molecular...
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Humana Press, 2006. — 1598 p. Series: Methods in Molecular Biology, № 346 Dictyostelium discoideum Protocols presents a comprehensive collection of cutting-edge molecular biological protocols for studying D. discoideum, with emphasis on its utility in the study of fundamental cellular processes including signal transduction, chemotaxis, cell motility, cytokinesis,...
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Springer, 2016. - 317 p. - Volume 1345 of Methods in Molecular Biology. This detailed volume focuses on methods for the characterization of aggregation processes that lead to the formation of amyloid fibrils and amyloid oligomers which feature in the etiology of a variety of human disorders collectively known as amyloidoses. The scope of the collection includes techniques for...
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Humana Press, 2012. — 564 p. — ISBN: 1617795542, 1617795569 Series: Methods in Molecular Biology, №850 Recent advances in genetics over the last quarter of a century, especially in molecular techniques, have dramatically reduced the cost of determining genetic markers and hence opened up a field of research that is increasingly helping to detect, prevent and/or cure many...
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Humana Press, 2012. — 564 p. — ISBN: 1617795542, 1617795569. Series: Methods in Molecular Biology, № 850 Recent advances in genetics over the last quarter of a century, especially in molecular techniques, have dramatically reduced the cost of determining genetic markers and hence opened up a field of research that is increasingly helping to detect, prevent and/or cure many...
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Humana Press, 2012. - 296 p. ISBN: 1627030107 Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers. In Genomic...
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New York: Humana Press, 2014. — 812 p. — (Methods in Molecular Biology 1076). Reflecting the expanding field’s need for reliable protocols, this volume offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy,...
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Humana Press, 2002. — 396 p. Series: Methods in Molecular Biology, №201. Contents Preface Library Synthesis and Quality Control Using a Noncovalent Protection Strategy to Enhance Solid-Phase Synthesis Quality Control of Solid-Phase Synthesis by Mass Spectrometry Preparation of Encoded Combinatorial Libraries for Drug Discovery Simple Tools for Manual Parallel Solid...
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Humana Press, 2015. — 356 p. — (Methods in Molecular Biology 1320). This volume includes comprehensive and up-to-date coverage of all nucleic-acid-specific steps used in X-ray crystallography, from macromolecule production to structure determination. Chapters dedicated to RNA preparation and crystallogenesis will be of interest to beginners, while chapters focused on data...
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Springer, 2017. — 224 p. — (Mvtthods in Molecular Biology 1663). — ISBN-10 1493972642. This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology...
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Humana Press, 2009. — 385 p. Series: Methods in Molecular Biology, № 559 The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research in the 21st century. In Apoptosis Methods and Protocols, highly experienced researchers in the field of apoptosis describe in step-by-step detail...
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New York: Humana Press, 2015. — 241 p. — (Methods in Molecular Biology 1291). This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics,...
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2nd Edition. — Springer Science+Business Media LLC, 2017. — 412 p. — (Methods in Molecular Biology 1606) — ISBN: 9781493969890. This second edition provides an update on technology and an accelerated tutorial to assist, students, entrepreneurs, new investigators, and established investigators who want to quickly become versed in, and immersed in, the entire process from discovery...
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Humana Press, 2015. — 245 p. Series: Methods in Molecular Biology, № 1242 This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Chapters focus on the cutting-edge methods to study in detail several complex aspects of cell expansion such as secretion, endocytosis and recycling, cellular signaling and...
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Humana Press, 2016 — 281p. — (Methods in Molecular Biology 1401) — ISBN: 978-1-4939-3375-4 (eBook), 978-1-4939-3373-0 (Hardcover). This volume presents an overview of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) structure and function. It then continues with methods for the analysis of these pathways including conventional enzymological assays,...
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Humana Press, 2008. — 452 p. Series: Methods in Molecular Biology, № 415 Immunologists today are interested in all of the diverse cell-types involved in host defense and have a deeper appreciation of the importance of innate immune mechanisms as a first line of protection against pathogens. This volume thus discusses the isolation and functional characterization of cells involved...
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Humana Press, 2007. — 494 p. — (Methods in Molecular Biology 380). The book is a comprehensive guide to the techniques currently used for culturing and characterising the cell types responsible for imposing self-tolerance and the experimental models employed to study their function both in vitro and in vivo. Provides up-to-date reviews of the literature on essential topics in...
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Humana Press, 2012. — 354 p. — (Methods in Molecular Biology 822). The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years. In this...
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Humana Press, 2016 — 298p. — (Methods in Molecular Biology 1402) — ISBN: 978-1-4939-3378-5 (eBook), 978-1-4939-3376-1 (Hardcover). This volume assembles a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA. Long Non-Coding RNAs:...
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Humana Press, 2017. — 279 p. — ISBN 10 1493972243, 13 978-1493972241. Series: Methods in Molecular Biology, № 1653 This volume presents a comprehensive overview of contemporary methods to analyze photorespiration in higher plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the...
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Humana Press, 2016 — 180p. — (Methods in Molecular Biology 1405) — ISBN: 978-1-4939-3393-8 (eBook), 978-1-4939-3391-4 (Hardcover). This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary...
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Humana Press, 2014 — 208p. — (Methods in Molecular Biology 1106) — ISBN: 978-1-62703-736-5 (eBook), 978-1-62703-735-8 (Hardcover). Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis:...
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Humana Press, 2014. — 800 p. Series: Methods in Molecular Biology, № 1149 In this volume, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful...
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Humana Press, 2014. — 267 p. Series: Methods in Molecular Biology, № 1145 The recent advent of molecular technologies has changed the way plant breeders identify and select their germplasm as genetic variation can now be assessed at the DNA level. This volume presents detailed guidelines and tutorials that suit different needs and capacity from small laboratories analyzing...
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Humana Press, 2018. - 412 p. - Volume 1716 of Methods in Molecular Biology. This volume looks at the latest methodologies used to study cellular metabolism with in silico approaches. The chapters in this book are divided into 3 parts: part I discusses tools and methods used for metabolic reconstructions and basic constraint-based metabolic modeling (CBMM); Part II explores...
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Humana Press, 2014 — 299p. — (Methods in Molecular Biology 1122) — ISBN: 978-1-62703-794-5 (eBook), 978-1-62703-793-8 (Hardcover). Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple...
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2nd edition. — Humana Press, 2014. — 258 p. — (Methods in Molecular Biology 1199). — ISBN: 978-1-4939-1280-3, 978-1-4939-1279-7. In Quantum Dots: Applications in Biology, Second Edition, expert researchers in the field detail consolidated approaches as well as new trends in the field. Organized into five parts, the first part comprises an introduction on Quantum Dots (QDs) as...
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Humana Press, 2013. — 385 p. Series: Methods in Molecular Biology, № 1027 The lipid-rich and otherwise challenging nature of many key tissues complicates many aspects of current research, and applications of the unique nature of lipoproteins and their biological effects has engendered unique and vital methodologies. In this volume experts in the field present a compendium of...
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6th edition. — Wiley-Blackwell, 2010. — 796 p. This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and...
  • №181
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Humana Press, 2004. — 501 p. Series: Methods in Molecular Biology, № 261 Proteins continuously interact with each other to determine cell fate. Consequently, an examination of just when such protein-protein interactions occur and how they are controlled is essential for understanding the molecular mechanism of biological processes, elucidating the molecular basis of diseases,...
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Humana Press, 2014. — 384 p. — (Methods in Molecular Biology 1100). The book is composed of 32 individual chapters that describe a variety of protocols to purify and analyze the activity of the individual complement components or pathways. It includes assays that describe detection of complement SNPs, clinical methods to evaluate complement system activation and data...
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Humana Press, 2013. — 534 p. Series: Methods in Molecular Biology, № 965 Cell senescence is the process whereby cells permanently lose the possibility to proliferate without undergoing cell death, and occurs in a plethora of distinct model organisms. In this volume, expert researchers in the field detail the methods that are now commonly used to study cell senescence, in model...
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Second Edition. — Humana Press, 2013. — 430 p. Series: Methods in Molecular Biology, № 998 Every cell in our bodies contains a great variety and number of permeability pathways for various organic and inorganic ions, water, metabolites, nutrients, and signalling molecules. Maintenance and precise control of gating within these pathways are fundamental principles of life as these...
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New York: Humana Press, 2015. — 443 p. With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not...
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Humana Press, 2016 — 434p. — (Methods in Molecular Biology 1400) — ISBN: 978-1-4939-3372-3 (eBook), 978-1-4939-3370-9 (Hardcover). This volume covers the latest protocols designed to identify and characterize TEs in genomes, ancient or recently inserted. Additionally, this book includes a series of protocols designed to understand how host genomes act to regulate the activity...
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Humana Press, 1995. - 412 p. ISBN B001DA0B6O Series: Methods in Molecular Biology, №44 Agrobacterium Protocols offers beginning and experienced researchers the most comprehensive collection of step-by-step protocols for the genetic manipulation of plants using Agrobacterium. The topics range from the maintenance of bacterial culture collections to aspects of the metabolism and...
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Humana Press, 2018. - 492 p. - Volume 1649 of Methods in Molecular Biology. This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA...
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Springer Science+Business Media LLC, 2017. — 268 p. — (Methods in Molecular Biology 1536) — ISBN: 1493966804 Oat is a cereal grain belonging to the genus Avenae which contains more than 20 species occurring at three ploidy levels, diploid, tetraploid, and hexaploid. The primary cultivated oat (Avena sativa L.) is a hexaploid (2n = 6x = 42) consisting of three genomes, AA, CC,...
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Humana Press, 2015 — 267p. — (Methods in Molecular Biology 1266) — ISBN: 978-1-4939-2272-7 (eBook), 978-1-4939-2271-0 (Hardcover). This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Chapters...
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Humana Press, 2010. — 263 p. Series: Methods in Molecular Biology, № 161 In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components....
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Humana Press, 2013. — 285 p. Series: Methods in Molecular Biology, № 1016 Over the last two decades there has been a growing interest in cyclic nucleotide research in plants with an emphasis on the elucidation of the roles of cGMP and cAMP. In this volume, expert researchers in the field detail many approaches to better understand the biological role of this important signaling...
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Humana Press, 2017. — 551 p. — (Methods in Molecular Biology 1486). The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation in vitro and...
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New York: Humana Press, 2015. — 195 p. The book explores on the most widely studied of the protein classes, known for their central role in several cellular functions and in a variety of human pathologies. Beginning with a section on activation and signaling of Receptor Tyrosine Kinases (RTKs), the book continues with chapters on aberrant expression of RTKs, trafficking and...
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Humana Press, 2013. — 330 p. Series: Methods in Molecular Biology, № 982 While many cytokines are known for their inflammatory action, there is a growing interest in the tissue-protective effects of some cytokines. The prototypic tissue-protective cytokine is EPO. Initially described as neuro-protective, it is beneficial in animal models of ischemic and other types of injury....
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Humana Press, 2018. - 448 p. - Volume 1688 of Methods in Molecular Biology. This volume covers state-of-the-art applications of solid-state and solution nuclear magnetic resonance( NMR) spectroscopy to study protein structure, dynamics and interactions. Chapters detail various aspects of data acquisition and processing, determination of the structure, multi-timescale dynamics of...
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2nd Edition. — Springer, 2014. — 383 p. Directed Evolution Library Creation: Methods and Protocols, Second Edition presents user-friendly protocols for both proven strategies and cutting-edge approaches for the creation of mutant gene libraries for directed evolution. As well as experimental methods, information on current computational approaches is provided in a user-friendly...
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Humana Press, 2004. — 200 p. — (Methods in Molecular Biology 285). — E-ISBN 1-59259-822-6. Preface Cell Cycle Control and Dysregulation Protocols focuses on emerging methodologies for studying the cell cycle, kinases, and kinase inhibitors. It addresses the issue of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation...
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Humana Press, 2016. — 235 p. — ISBN: 1493963651 Series: Methods in Molecular Biology, №1477 This volume focuses on the developmental areas of biopesticides: production, formulation, application and field efficacy. Chapters guide readers through methods and techniques on environmental, mammalian, safety, and registration. Written in the highly successful Methods in Molecular...
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Springer Science+Business Media, 2013. — 565 p. — ISBN: 1627034463 Series: Methods in Molecular Biology, №1019 With the detailed genomic information that is now becoming available, we have a plethora of data that allows researchers to address questions in a variety of areas. Genome-wide association studies (GWAS) have become a vital approach to identify candidate regions...
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Humana Press, 2013. — 467 p. — (Methods in Molecular Biology 1030). Although antiviral drugs have been successfully developed for some viral diseases, there remains a clear, unmet medical need to develop novel antiviral agents for the control and management of many viruses that currently have no or limited treatment options as well as a need to overcome the limitations associated...
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Humana Press, 2013. — 363 p. Series: Methods in Molecular Biology, № 1011 It is now well established that jasmonates, originally identified as the major component of jasmine scent, play a universal role in the plant kingdom and are involved in the regulation of diverse aspects of plant biology, including growth, development, metabolism, and interaction with the environment. In...
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Humana Press, 2014. — 547 p. Series: Methods in Molecular Biology, №1097 The existence of noncoding RNA genes (ncRNAs) was proposed simultaneously with protein coding genes in 1961 by Jacob and Monod. Since then, a substantial focus has been on protein coding genes, while the area of ncRNA evolved more slowly and received less attention even though major breakthroughs were...
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Humana Press, 1994. - 492 p. ISBN 0896032892 Series: Methods in Molecular Biology, №29 Contents Mitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution. Chromosome Preparation from Hematological Malignancies. Meiotic Chromosome Preparation. Preparation of Chromosomes for Scanning Electron Microscopy. Immortalized Cell Lines: Chromosome...
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Humana Press, 2004. — 445 p. Series: Methods in Molecular Biology, № 263 The study of RNA interference, editing, and modification has led to major shifts in our understanding of how genes are expressed and regulated. In RNA Interference, Editing, and Modification: Methods and Protocols, hands-on experimentalists describe in detail the protocols and assays they have developed...
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Second Edition. — Humana Press, 2001. — 182 p. Series: Methods in Molecular Biology, № 167 Since the publication of the first edition of DNA Sequencing Protocols in 1993, major advances in its technology have made PCR-based semiautomated fluorescent sequencing the norm and enabled the complete sequencing of complex genomes. In DNA Sequencing Protocols, 2nd Edn., Colin Graham...
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Humana Press, 1993. — 354 p. ISBN 0896032361 Series: Methods in Molecular Biology, №19 Expert practitioners combine the traditional basic techniques of membrane isolation and analysis with modern advances in molecular biology, immunology, and clinical sciences to devise analytical techniques that are successful for expert and novice alike. The techniques are used in the...
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Humana Press, 1993. — 352 p. ISBN 0896032507 Series: Methods in Molecular Biology, №27 Crystallization of Membrane Proteins for X-Ray Analysis Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination Biochemical Probes of Topography Antipeptide Antibodies The Production of Monoclonal Antibodies to Membrane Proteins
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Humana Press, 2000. — 274 p. Series: Methods in Molecular Biology, № 34 In this volume James Gray and Ulrich Desselberger have assembled a comprehensive collection of established and cutting-edge methods for studying and illuminating the structure, molecular biology, pathogenesis, epidemiology, and prevention in animal models of infection with rotaviruses, an important cause of...
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Humana Press, 1994. — 366 p. Series: Methods in Molecular Biology, № 24 DNA sequencing has become increasingly efficient over the years, resulting in an enormous increase in the amount of data gen- ated. In recent years, the focus of sequencing has shifted, from being the endpoint of a project, to being a starting point. This is especially true for such major initiatives as...
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Humana Press, 1994. — 424 p. Series: Methods in Molecular Biology, № 25 DNA sequencing has become increasingly efficient over the years, resulting in an enormous increase in the amount of data gener­ ated. In recent years, the focus of sequencing has shifted, from being the endpoint of a project, to being a starting point. This is especially true for such major initiatives as...
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Humana Press, 1993. — 384 p. ISBN 0896032485 Series: Methods in Molecular Biology, №23 DNA Sequencing Protocols provides you with the knowledge to become a sequencing expert. An all-star cast of investigators covers virtually all aspects, including developments in cycle sequencing, sequencing PCR products, sequencing lambda and cosmids, multiplex sequencing, direct blotting...
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Humana Press, 2003. — 433 p. As more and more plant genomes are sequenced, plant researchers have been inundated with an avalanche of novel methodologies to identify the function of tens of thousands of plant genes. In Plant Functional Genomics, Erich Grotewold has assembled a team of leading plant scientists to describe in detail the most commonly used methods for investigating...
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Humana Press, 2016 — 2nd Ed. — 256p. — (Methods in Molecular Biology 1381) — ISBN: 978-1-4939-3204-7 (eBook), 978-1-4939-3203-0 (Hardcover). This second edition volume brings together the experiences of leading scientists in the discipline of cancer gene profiling. Because cancer genes can be profiled in many different ways, Cancer Gene Profiling: Methods and Protocols, Second...
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Humana Press, 2004. - 428 p. Series: Methods in Molecular Biology, №243 Prominent experts from around the world detail the chromatographic and electroseparation techniques they have developed for chiral separations on an analytical scale. Described in step-by-step detail to ensure successful experimental results, the procedures are presented as either general methods or as...
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Humana Press, 2016. — 315 p. — ISBN 1493967290. Series: Methods in Molecular Biology, № 1546 This detailed book arrives as there is an increasing need for multiplex biomarker readouts for improved clinical management and to support the development of new drugs by pharmaceutical companies, due to continuous technical developments and new insights into the high complexity of...
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Humana Press, 1999. — 213 p. — Methods in Molecular Biology, Vol. 127. In Molecular Methods in Developmental Biology: Xenopus and Zebrafish, Matthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques,...
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Humana Press, 2017. — 277 p. — (Methods in Molecular Biology 1528). This volume provides a comprehensive list of protocols for molecular biologists, biochemists and geneticists. Chapters cover protocols that further the study into protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the...
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Humana Press, 2017. — 301 p. — (Methods in Molecular Biology 1540). This volume serves as a reference for the dissemination of advances made in the study of Hepatitis B Virus (HBV). Hepatitis B Virus: Methods and Protocols details protocols and techniques ranging from cell culture studies to in vivo and clinical immunology. The chapters in this book discuss treatments of in vitro...
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2nd Ed. — Humana Press, 2016 — 226p. — (Methods in Molecular Biology 1375) — ISBN: 978-1-4939-3173-6 (eBook), 978-1-4939-3172-9 (Hardcover). This volume covers a large area, from the description of methodologies for data analysis to the real application. Chapters focus on methodologies for preprocessing of microarray data, a survey of miRNA Data analysis, Cloud-based...
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Humana Press, 2018. — 311 p. — (Methods in Molecular Biology 1850). — ISBN 978-1-4939-8730-6 (eBook). About this book This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese...
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Humana Press, 2017. — 210 p. — (Methods in Molecular Biology 1589). This detailed volume focuses on population epigenetics, which is of increasing interest to policy makers searching for explanations for complex epidemiological observations and conceptual models on which to base interventions. With a concentration on DNA methylation, the emphasis is on the most commonly studied...
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Humana Press, 2003. — 276 p. Series: Methods in Molecular Biology. Vol. 117 General Preparation of Material and Staining of Sections (Heather A. Davies) Negative Staining of Thinly Spread Biological Particulates (J. Robin Harris) Preparation of Thin-Film Frozen-Hydrated/Vitrified Biological Specimens for Cryoelectron Microscopy (J. Robin Harris and Marc Adrian) The...
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Humana Press, 2013. — 279 p. Series: Methods in Molecular Biology, № 981 Thousands of proteins have been identified to be acetylated. Immense research power has been dedicated to experiments to solve the biological implications of each and every protein acetylation. Two particular sites of protein acetylation have been described intensively: the N-terminal methionine residue of a...
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2nd Edition. — Humana Press, 2015. — 236 p. — ISBN: 9781493916795 Series: Methods in Molecular Biology, № 1228 This volume presents detailed, recently-developed protocols ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well-explored aspects of the nucleus. The methods described include new...
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Humana Press, 2008. — 323 p. — (Methods in Molecular Biology 463). — ISBN: 978-1-59745-406-3 (eBook), 978-1-61737-863-8 (Softcover), 978-1-58829-977-2 (Hardcover). Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development...
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Humana Press, 2008. — 444 p. — (Methods in Molecular Biology 464). — ISBN: 978-1-60327-461-6 (eBook), 978-1-61737-898-0 (Softcover), 978-1-60327-460-9 (Hardcover). Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development...
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Humana Press, 2017. - 431 p. Volume 1548 of Methods in Molecular Biology. This volume provides basic and advanced laboratory protocols for the non-specialist and the experienced researcher. Chapters are divided into three parts covering synthesis and characterization of AMPs, studying the interaction of AMPs with model systems or bacteria, and assaying selected biological...
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New Jersey: John Wiley & Sons, Inc., 2016. — 412 p. As a modern composite scientific discipline, Cell Biology has expanded and moved forward rapidly in recent years. Cell Biologists now require a wide range of techniques, including those of analytical biochemistry and microscopy in all its diverse forms. These are often used alongside the techniques of molecular biology and...
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Humana Press, 1996. — 512 p. — (Methods in Molecular Biology 58). An essential core collection of the latest molecular and genetic techniques for cloning, subcloning, sequencing, PCR, protein expression, and much more. Each protocol represents a time-tested, step-by-step recipe that creates an understanding of the procedure, easily reproducible results, and confidence that the...
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Humana Press, 1994. - 388 p. ISBN 0896032582 Series: Methods in Molecular Biology, №31 Contents Basic Recombinant DNA Techniques:Transformation of Bacteria by Electroporation. Direct Cloning of lgt11 cDNA Inserts into a Plasmid Vector. PCR Cloning Using T- Vectors. Thermal Cycle Dideoxy DNA Sequencing. Ordered Deletions Using Exonuclease III. In Vitro Mutagenesis:...
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Humana Press, 2013. — 271 p. — (Methods in Molecular Biology 1017). The genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences. Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine. Tandem repeats have not only been implicated in...
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4th Edition. - Humana Press, 2018. - 495 p. - Volume 1678 of Methods in Molecular Biology. This fourth edition volume expands on the previous editions by presenting readers with new and emerging methodologies in cytometry. The chapters in this book cover various topics such as quantifying surface and intracellular protein biomarkers, intracellular cytokine staining, apoptosis...
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2nd Edition. - Humana Press, 2004. - 425 p. Series: Methods in Molecular Biology, №263 In addition to providing chapters on the theoretical and practical aspects of flow cytometry, this book contains protocols used specifically for immediate use in the diagnostic and experimental measurements enabled by modern flow cytometric instrumentation. Research protocols cover cell...
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Humana Press, 2015. — 213 р. Series Methods in Molecular Biology, №1203 Mass Spectroscopy Imaging (MSI) has emerged as an enabling technique to provide insight into the molecular entities within cells, tissues and whole-body samples and to understand inherent complexities within biological metabolomes. In Mass Spectrometry Imaging of Small Molecules: Methods and Protocols,...
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Humana Press, 2018. — 437 p. — (Methods in Molecular Biology 1705). This volume looks at modern computational strategies and techniques used in GPCR drug discovery including structure and ligand-based approaches and cheminformatics. The chapters in this book describe how these approaches can be applied to address key drug discovery issues, such as receptor structure modelling,...
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New York: Humana Press, 2015. — 341 p. The book provides a unique compendium of currently applied methods to explore plasmodesmata (PD). Opening with overviews of PD structure and their function during development as well as the role of viruses as models for addressing cellular mechanisms in macromolecular trafficking and movement through PD, the book continues with detailed...
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Humana Press, 2012. — 657 p. — (Methods in Molecular Biology 816). Studies over the past decade have continued to bring tremendous advances to our understanding of bone biology. New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength. In this volume expert researchers in the...
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3rd Edition. - Humana Press, 2004. - 365 p. - In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of the major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new...
  • №240
  • 6,63 МБ
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Humana Press, 2015. — 331 p. Series: Methods in Molecular Biology, № 1263 This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential components of the chemical biology tool discovery process, the book is...
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Humana Press, 2010. — 443 p. Series: Methods in Molecular Biology, № 655 The beauty and variety of plants has fascinated mankind for thousands of years, and this fascination continues today as plant developmental biologists discover mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant’s life. In Plant...
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Humana Press, 2006. — 249 p. Series: Methods in Molecular Biology, № 339 Microchip capillary electrophoresis, a new and emerging technology that can rapidly analyze biological compounds as small as or smaller than a single cell, will have a major impact on biological sciences in the next 10 to 20 years. In Microchip Capillary Electrophoresis: Methods and Protocols, leading...
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Humana Press, 2014. — 300 p. Series: Methods in Molecular Biology, № 1099 ​In this volume, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes. Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described. Tools for the isolation, characterization and...
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Humana Press, 2014 — 427p. — (Methods in Molecular Biology 1126) — ISBN: 978-1-62703-980-2 (eBook), 978-1-62703-979-6 (Hardcover). Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of...
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  • 7,09 МБ
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Humana Press, 2016 — 2nd Ed. — 292p. — (Methods in Molecular Biology 1397) — ISBN: 978-1-4939-3353-2 (eBook), 978-1-4939-3351-8 (Hardcover). This second edition expands upon the previous volume with additional emphasis on recent innovation in basic renal research and has a more holistic approach on associated disorders such as complications associated with peritoneal dialysis,...
  • №246
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Humana Press, 2014. — 261 p. Series: Methods in Molecular Biology, № 1056 In this volume, expert researchers in the field detail many of the techniques used for identification of bioactive compounds from a large selection of fields in plant biology including plant pathogenesis, immune responses, small RNA processing, endomembrane trafficking, lipids, plant hormone signaling and...
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  • 4,45 МБ
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Humana Press, 2017. — 234 p. — (Methods in Molecular Biology 1545). This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing...
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Humana Press, 2014 — 328p. — (Methods in Molecular Biology 1130) — ISBN: 978-1-62703-989-5 (eBook), 978-1-62703-988-8 (Hardcover). Skeletal Development and Repair: Methods and Protocols is a compilation of a variety of skeletal research protocols utilizing the laboratory mouse as the platform for surgical manipulation and/or transplantation as well as the source of tissues...
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Humana Press, 2014. — 295 p. Series: Methods in Molecular Biology, № 1166 The book details many of the methods and protocols commonly used to study plant cold acclimation and freezing tolerance, breeding, genetics, physiology or molecular biology, or any combination of these specialties. Chapters focus on interdisciplinary approaches, experimental methods, and concepts from...
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Humana Press, 2015 — 216p. — (Methods in Molecular Biology 1318) — ISBN: 978-1-4939-2742-5 (eBook), 978-1-4939-2741-8 (Hardcover). This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and...
  • №251
  • 9,95 МБ
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Humana Press, 2017. — 392 p. — (Methods in Molecular Biology 1628). This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology. After an...
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  • 11,08 МБ
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Humana Press, 2016 — 186p. — (Methods in Molecular Biology 1409) — ISBN: 978-1-4939-3515-4 (eBook), 978-1-4939-3513-0 (Hardcover). This volume explores the evolution of bacterial cancer therapy and describes the modern techniques used in therapy today. The chapters in this book cover a broad range of topics such as the development of tumor-targeting Salmonella typhimurium...
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  • 7,06 МБ
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Humana Press, 2003. — 400 p. — (Methods in Molecular Biology 209). The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second...
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  • 8,21 МБ
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Humana Press, 2000. — 341 p. Series: Methods in Molecular Biology, № 145 The interest of investigators across a broad spectrum of scientific dis- plines has been steadily stimulated by the field of bacterial toxin research, an area that makes use of a large variety of biological, chemical, physicochemical, and medically oriented approaches. Researchers studying bacterial...
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  • 2,18 МБ
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2nd Edition. — Springer Science+Business Media LLC, 2017. — 204 p. — ISBN: 9781493969562. Series: Methods in Molecular Biology, № 1600 This second edition volume expands on the previous editions with detailed protocols on (cyano) bacterial and mold fungus toxins, and a specific focus on Gram-negative endotoxins (lipopolysaccharides). The chapters in Microbial Toxins: Methods and...
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Humana Press, 2013. — 314 p. Series: Methods in Molecular Biology, № 957 Mammalian oocytes occupy a critical nexus in reproduction as they not only contribute half the genetic makeup of the embryo but also provide virtually all of the cytoplasmic building blocks required for sustaining embryogenesis. The journey that transforms a primordial germ cell into a mature oocyte (or egg)...
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Humana Press, 2000. — 403 p. — (Methods in Molecular Biology 32). With the molecular mechanisms underlying the various forms of muscular dystrophy now rapidly clarifying, precise diagnosis has become a reality, and even a requirement in clinical practice. In Muscular Dystrophy: Methods and Protocols, Katherine Bushby and Louise Anderson have assembled an outstanding collection of...
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Humana Press, 2015. — 366 p. — (Methods in Molecular Biology 1348). This extensive volume covers basic and advanced aspects of peptide antibody production, characterization and uses. Although peptide antibodies have been available for many years, they continue to be a field of active research and method development. For example, peptide antibodies which are dependent on...
  • №259
  • 12,52 МБ
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Humana Press, 1993. - 379 p. ISBN 0896032264 Series: Methods in Molecular Biology, №14 Contents A General Strategy for Glycoprotein Oligosaccharide Analysis. Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography. Sequence and Linkage Analysis of N- and O-Linked Glycans by Fast Atom Bombardment Mass Spectrometry. Gas...
  • №260
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Humana Press, 1995. — 278 p. ISBN 0896032973 Series: Methods in Molecular Biology, №46 Antibiotic Resistance Profiling Bacteriocin Typing Bacteriophage Typing The Analysis of Bacterial Proteins by SDS Polyacrylamide Gel Electrophoresis Lipopolysaccharide Chemotyping Multilocus Enzyme Electrophoresis Fast Atom Bombardment-Mass Spectrometry
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  • 2,28 МБ
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Springer, 2017. — 283 p. — (Methods in Molecular Biology 1617). This thorough volume provides an in-depth introduction to and discussion of microRNAs (miRs) and their targets, miR functions, and computational techniques applied in miR research, thus serving the need for a comprehensive book focusing on miR target genes, miR regulation mechanisms, miR functions performed in various...
  • №262
  • 5,79 МБ
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New York: Humana Press, 2015. — 535 p. — (Methods in Molecular Biology 1220). The book expands upon the previous edition with current, detailed reviews that cover topics of interest to mast cell neophytes and cognoscenti alike. With new chapters on methods for the enumeration of tissue mast cells and isolation of mature mast cells and mast cell progenitors from mammalian tissues,...
  • №263
  • 15,16 МБ
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Humana Press, 2004. - 409 p. Series: Methods in Molecular Biology, №296 This collection of cutting–edge techniques for the study of the eukaryotic cell cycle and its key regulatory molecules includes overviews of cell cycle regulatory mechanisms in many major research organisms. Described in step-by-step detail, these readily reproducible methods enable fundamental research on...
  • №264
  • 8,71 МБ
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Humana Press, 2015. — 237 p. Series: Methods in Molecular Biology, № 1255 This volume presents the most recent studies on mRNA polyadenylation in plants. Chapters are divided into three sections covering recent development of the use of bioinformatics tools in the field. Numerous molecular, biochemical, and methods used to characterize polyadenylation sites on a genome-wide scale....
  • №265
  • 7,01 МБ
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Humana Press, 2011. - 447 p. - Methods in Molecular Biology, Volume 726. - Due to their unique size-dependent properties, nanomaterials have the potential to revolutionize the detection, diagnosis, and treatment of disease by offering superior capabilities compared to conventionally-used materials. Biomedical Nanotechnology: Methods and Protocols brings together experts from a...
  • №266
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Humana Press, 2018. - 556 p. - Volume 1701 of Methods in Molecular Biology. This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3)...
  • №267
  • 10,75 МБ
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Humana Press, 1993. — 443 p. ISBN 0896032396 Series: Methods in Molecular Biology, №21 Culturing and Biological Cloning of Trypanosoma brucei Culturing and Biological Cloning of Trypanosoma cruzi In Vitro Cultivation and Biological Cloning of Leishmania Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages) Suitable for...
  • №268
  • 3,76 МБ
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Humana Press, 2005. — 225 p. — (Methods in Molecular Biology 311). — ISBN 1-59259-957-5. History and Overview Pharmacogenomics: Historical Perspective and Current Status Functional Analysis of Gene Variation Transfection Assays With Allele-Specific Constructs: Functional Analysis of Udp-glucuronosyltransferase Variants Snapshot of the Allele-Specific Variation in Human Gene...
  • №269
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Humana Press, 2001. — 514 p. ISBN 978-0-89603-759-5 Series: Methods in Molecular Biology, №171 Isolation of Proteoglycans from Cell Cultures and Tissues. (Masaki Yanagishita) Isolation of Proteoglycans from Tendon. (Kathryn G. Vogel and Julie A. Peters) Purification of Proteoglycans from Mineralized Tissues. (Neal S. Fedarko) Purification of Perlecan from Endothelial...
  • №270
  • 3,09 МБ
  • дата добавления неизвестна
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Humana Press, 1993. - 260 p.ISBN: 9780896032545 Series: Methods in Molecular Biology, №28 Contents About Nonradioactive Nucleic Acid Detection Isolation of DNA from Plants Isolation of High-Molecular-Weight DNA from Animal Cells Restriction Enzyme Digestion, Gel Electrophoresis, and Vacuum Blotting of DNA to Nylon Membranes Isolation of Plant RNA Isolation of Total...
  • №271
  • 1,96 МБ
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Humana Press, 2004. — 408 p. Series: Methods in Molecular Biology, № 269 Recombinant vaccinia viruses have become important in recent years, both as protein expression vectors, and-in the age of bioterrorism-as powerful tools for developing new smallpox vaccines, diagnostics, and understanding of poxvirus pathogenesis. In Vaccinia Virus and Poxvirology: Methods and Protocols,...
  • №272
  • 7,08 МБ
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Humana Press, 2018. — 175 p. Series: Methods in Molecular Biology, № 1763 This detailed volume presents a number of chapters that will aid researchers in jumping the hurdle preventing the popularization of advanced intravital imaging technology usage, namely the complicated experimental protocols. Leading researchers undertaking imaging studies in the field of bone and immune...
  • №273
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Humana Press, 2016 — 275p. — (Methods in Molecular Biology 1434) — ISBN: 978-1-4939-3652-6 (eBook), 978-1-4939-3650-2 (Hardcover). Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in...
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  • 6,94 МБ
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Humana Press, Springer Science + Business Media, 2008. — 251 p. Series: Methods in Molecular Biology, №437 In this concise and systematic book, a team of experts select the most important, cutting-edge technologies used in drug delivery systems. They take into account significant drugs, new technologies such as nanoparticles, and therapeutic applications. The chapters...
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  • 3,27 МБ
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New York: Humana Press, 2015. — 199 p. The book highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication. Human rhinoviruses are the major cause of common colds as well as...
  • №276
  • 5,72 МБ
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Humana Press, 2015. — 414 p. — (Methods in Molecular Biology 1271). — ISBN: 978-1-4939-2330-4 (eBook), 978-1-4939-2329-8 (Hardcover). This volume explores the technological improvements in protein engineering, expression, purification, and crystallization of several rhodopsin photoactive intermediates, thus increasing our understanding of rhodopsin activation. The first...
  • №277
  • 13,87 МБ
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Humana Press, 1995. - 394 p. ASIN B001F7490O Series: Methods in Molecular Biology, №34 Contents Overview of Antibody Use in Immunocytochemistry. Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration. Purification of Antibodies Using Ion-Exchange Chromatography Purification of Antibodies Using Affinity Chromatography Purification of Antibodies...
  • №278
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Humana Press, 2018. — 476 p. — (Methods in Molecular Biology 1767). — ISBN: 978-1-4939-7774-1 This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains,...
  • №279
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Humana Press, 2013. — 541 p. Series: Methods in Molecular Biology, № 949 Microfluidic techniques are becoming widely incorporated into medical diagnostic systems due to the inherent advantages of miniaturization. In this volume researchers in the field detail methods and protocols covering subjects such as microfluidic device fabrication, on-chip sample preparation, diagnostic...
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Humana Press, 2017. — 435 p. — (Methods in Molecular Biology 1487). This volume provides a collection of techniques and approaches for the study of ERK signaling. It begins with a historical perspective of genetic and molecular discoveries, followed by chapters covering specific topics in a broad range of experimental systems, including in vitro assays of EGFR and ERK activities;...
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2nd Edition. - Humana Press, 2018. - 397 p. - Volume 1683 of Methods in Molecular Biology. This second edition details some of the recent trends in HCS/HCA/HCI. New and updated chapters guide readers through methods that utilize reagents and kits that have been developed to measure cells and subpopulation classifications, control measures, overviews of the data handling issues...
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Humana Press, 1993. — 274 p. ISBN 0896032329 Series: Methods in Molecular Biology, №22 This superb survey of the possible applications of physiochemical techniques to the analysis of biological macromolecules is designed for the practicing biochemist or biologist who wants to use the technique but lacks the experience. The contributors emphasize practical aspects, such as...
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Humana Press Inc.,1993. – 406 p. – ISBN: 9780896032156. Series: Methods in Molecular Biology, №17 The main strength of this volume...is the extensive attention paid to the pragmatic and mundane issues that are only gleaned with difficulty from the literature, yet are absolutely critical for success. Those contemplating initiating studies employing these physical methods would...
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Humana Press, 1995. — 457 p. ISBN 089603321X Series: Methods in Molecular Biology, №49 In Plant Gene Transfer and Expression Protocols, leading experts present the latest step-by-step recipes for introducing genes into model and agricultural plants and for studying gene activity using reporter enzyme assays, RNA techniques, and immunological methods. The book contains not...
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Humana Press, 2014. — 297 p. Series: Methods in Molecular Biology, № 1157 Listeria monocytogenes is still a major threat to public health. A new book in the Methods in Molecular Biology series addresses its titular pathogen with protocols and methodologies used in research to gain a better understanding of Listeria at a molecular level. The topics covered include sampling in order...
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Humana Press, 2013. — 335 p. — (Methods in Molecular Biology 1041). Key discoveries concerning the different biological functions of microglia in health and disease have attracted scientists from various fields. In this volume, expert researchers in the field detail methods for selection of the key cellular, molecular and biochemical techniques that are used in studying the many...
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Humana Press, 2015. — 314 p. — ISBN: 1493931059, 9781493931057 Series: Methods in Molecular Biology, № 1362 This valuable collection aims to provide a collection of frequently used statistical methods in the field of proteomics. Although there is a large overlap between statistical methods for the different ‘omics’ fields, methods for analyzing data from proteomics...
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Humana Press, 2013. — 325 p. Series: Methods in Molecular Biology, № 964 Dopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery. In this volume experts and key figures within the field provide detailed protocols on...
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Humana Press, 2005. — 300 p. — (Methods in Molecular Biology 302). — eISBN 1-59259-903-6. Elispot Assay Setup And Data Analysis The Elispot Assay: A Personal Retrospective Chemistry And Biology Of The Elispot Assay Membranes And Membrane Plates Used In Elispot Standardization And Validation Issues Of The Elispot Assay A Cell-Detachment Solution Can Reduce Background Staining In...
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2nd Edition. - Humana Press, 2017. — 288 p. — (Methods in Molecular Biology 1554). This second edition volume provides detailed protocols that address the challenges of signal-transduction IHC. This book delves into chapters that discuss the nature of signal transduction phenomena and approaches to making phosphor-specific antibodies, as well as numerous bona fide methods methods...
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Humana Press, 2013. — 353 p. Series: Methods in Molecular Biology, № 1006 Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems. The book brings together experts in the field to cover this significant area of research....
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Humana Press, 2013. — 229 p. Series: Methods in Molecular Biology, № 1036 Embryonic stem cells and adult stem cells are the two major types of stem cells that have been used for experimental and clinical studies. Embryonic stem cells are totipotent cells that have the capability to differentiate into any type of cell in the body. In this volume expert researchers in the field...
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Humana Press, 2012. - 435 p. Over the last decade Life Science has undergone an accelerated evolution, culminating in the -omics era characterized by the development of a multitude of high throughput methods that are becoming more routinely applied in biochemistry labs. In Functional Genomics: Methods and Protocols, Second Edition expert researchers in the field detail many of...
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3rd Ed. — Humana Press, 2017. — 385 p. — (Methods in Molecular Biology). — ISBN 10 1493972308, 13 978-1493972302. Reflecting developments in genome editing, this third edition volume fully updates a collection of key techniques for the study of functional genomics. The book is broken up into sections on bioinformatics, DNA, RNA, and protein analysis, as well as a closing section...
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Humana Press, 2012. — 240 p. — (Methods in Molecular Biology 865). Reports of influenza-like illnesses date back to the Middle Ages, and outbreaks of influenza likely afflicted humans long before that. Over the last half century, influenza virus research has led to the development of two classes of antivirals – ion channel and neuraminidase inhibitors. Recently, a method of the...
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Humana Press, 2017. - 284 p. Volume 1500 of Methods in Molecular Biology. This detailed volume presents a comprehensive technical overview of DNA nanotechnology with an emphasis on 3D DNA nanostructure design and applications. Coverage spans from basic design principles for DNA and RNA nanostructures to their cutting-edge applications in a variety of fields, with the book divided...
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Humana Press, 2017. — 234 p. Series: Methods in Molecular Biology, № 1549 This thorough book covers the most recent proteomics techniques, databases, bioinformatics tools, and computational approaches that are used for the identification and functional annotation of proteins and their structure. The most recent proteomic resources widely used in the biomedical scientific community...
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2nd Edition. — Springer Science+Business Media, New York, USA, 2017. — 491 p. — (Methods in Molecular Biology 1525) — ISBN: 1493966200 This second edition provides updated and expanded chapters covering a broad sampling of useful and current methods in the rapidly developing and expanding field of bioinformatics. Bioinformatics, Volume I: Data, Sequence Analysis, and Evolution,...
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2nd ed. — Humana Press, 2017. — 422 p. — (Methods in Molecular Biology). — ISBN 10 1493966111. — ISBN 13 978-1493966110. This second edition provides updated and expanded chapters covering a broad sampling of useful and current methods in the rapidly developing and expanding field of bioinformatics. Bioinformatics, Volume II: Structure, Function, and Applications, Second...
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Humana Press, 1995. - 301 p. ISBN 0896032981 Series: Methods in Molecular Biology, №41 This collection of self-contained, "hands-on" descriptions of established laboratory protocols concentrates on receptor-mediated cell signaling, with particular attention devoted to those receptors that are part of the G-protein-linked superfamily. It offers those already working in the...
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Humana Press, 1992. - 338 p. ISBN 0896032132 Series: Methods in Molecular Biology Series , №11 Contents Thiophilic Adsorption Chromatography. Immobilized Metal Ion Affinity Chromatography. Histidine Ligand Affinity Chromatography. Boronic Acid Matrices for the Affinity Purification of Glycoproteins and Enzymes. Calcium-Dependent Hydrophobic Interaction...
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Humana Press, 2015. — 413 p. — (Methods in Molecular Biology 1249). — ISBN 149392012X, 9781493920129. Includes cutting-edge methods and protocols for researching cervical cancer and high-risk HPV types Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Representing the most relevant procedures and...
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Humana Press, 2011. - 336 p. Series: Methods in Molecular Biology, №671 In Biological Microarrays: Methods and Protocols, expert researchers explore exciting new developments in the field, providing a comprehensive approach to biological microarrays that conveys not only the state-of-the-art fundamentals, but also includes applications of the most innovative methods....
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Humana Press, 2016 — 408p. — (Methods in Molecular Biology 1408) — ISBN: 978-1-4939-3512-3 (eBook), 978-1-4939-3510-9 (Hardcover). This volume provides researchers with up-to-date protocols and takes a close look at current research and promising applications. Optogenetics: Methods and Protocols contains a collection of recently developed technical protocols on optogenetic...
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Humana Press, 2014. — 297 p. Series: Methods in Molecular Biology, № 1150 The book collects techniques used to increase our understanding of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. The volume opens with a section on next-generation sequencing library preparation and data analysis. Continuing with a collection of...
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3rd Edition. - Springer, 2014. - 267 p. - Volume 1137 of Methods in Molecular Biology. - Protein Structure Prediction, Third Edition expands on previous editions by focusing on software and web servers. With new chapters that provide instructions on how to use a computational method with examples of prediction by the method. Written in the highly successful Methods in Molecular...
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Humana Press, 2012. - 329 p. - Super secondary structure(SSS) helps to understand the relationship between primary and tertiary structure of proteins. In Protein Supersecondary Structure: Methods and Protocols expert researchers in the field detail the usefulness of the study of super secondary structure in different areas of protein research. This is done through four main...
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Humana Press, 2017. — 289 p. — (Methods in Molecular Biology 1497). This volume aims to present a representative cross-section of modern experimental approaches relevant to Plant Hormone Biology, ranging from relatively simple physiological to highly sophisticated methods. Chapters describe physiological, developmental, microscopy-based techniques, measure hormone contents, and...
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Humana Press, 2013. — 475 p. Series: Methods in Molecular Biology, № 974 Biological membranes are the essential structuring elements of all living cells. Many enzymatic reactions take place at the membrane-water interface. To gain detailed insight into membrane properties, it is therefore of great importance to understand the complex nature of the interactions of membrane proteins...
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Humana Press, 2015. — 230 p. — (Methods in Molecular Biology 1289). — ISBN: 978-1-4939-2486-8 (eBook), 978-1-4939-2485-1 (Hardcover). This volume covers the techniques necessary for a successful fragment-based drug design project, beginning from defining the problem in terms of preparing the protein model, identifying potential binding sites, and the consideration of various...
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Humana Press, 1994. - 425 p. ISBN 0896032566 Series: Methods in Molecular Biology, №30 Contents DNase I Footprinting Footprinting with Exonuclease III Hydroxyl Radical Footprinting Hydroxyl Radical Interference 1,10-Phenanthroline-Copper Ion Nuclease Footprinting of DNA-Protein Complexes in Situ Following Mobility-Shift Electrophoresis Assays Identification of...
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Springer Science+Business Media, 2016. — 769 p. — (Methods in Molecular Biology 1462). — ISBN: 1493938142 This volume discusses experimental brain injury models that contain valuable information carefully chosen to widen the researchers’ horizon about neurotrauma. Injury Models of Central Nervous System: Methods and Protocols contains relevant experimental design approaches...
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Springer Science+Business Media, 2016. — 769 p. — (Methods in Molecular Biology 1462). — ISBN 1493938142. This volume discusses experimental brain injury models that contain valuable information carefully chosen to widen the researchers’ horizon about neurotrauma. Injury Models of Central Nervous System: Methods and Protocols contains relevant experimental design approaches...
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2nd Ed. — Humana Press, 2017. — 423 p. — (Methods in Molecular Biology 1598). — ISBN 10 1493969501, ISBN 13 978-1493969500. Preview ebook This new edition provides updated and novel protocols of neuroproteomics methods that encompass both global-scale as well as targeted and specialized topics, which are timely additions for the molecular and phenotypic analysis of the central...
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Humana Press, 2013. — 353 p. Series: Methods in Molecular Biology, №951 Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field...
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Humana Press, 2004 — 355 p. — e-ISBN: 1-59259-804-8 Series: Methods in Molecular Biology, № 277 Contents Introduction Mouse Models of Triplet Repeat Diseases Analysis of triplet repeat DNAs and RNAs Analysis of Triplet Repeat Replication by Two-Dimensional Gel Electrophoresis Genetic Analysis for Triplet Repeat Instability in Yeast Detection and Isolation of Trinucleotide Repeat...
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Humana Press, 2012. — 255 p. — (Methods in Molecular Biology 904). Stem cell mobilization represents a transient increase in the levels of circulating stem and progenitor cells. In this volume expert researchers in the field detail cell mobilization methodology and recent developments in the field for basic and biomedical research community. Specifically clinical hematopoietic...
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Humana Press, 2014. — 279 p. Series: Methods in Molecular Biology, № 1171 Mitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors. The main aim of Plant MAP Kinases: Methods and Protocols is to provide...
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Humana Press, 2013. — 283 p. Series: Methods in Molecular Biology, № 1024 microRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In this volume expert researchers in the field detail recent advances in the isolation, purification...
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2nd Edition. — Springer Science+Business Media, New York, 2017. — 256 p. — (Methods in Molecular Biology 1456) — ISBN-10: 1489977066 This volume provides a variety of protocols to analyze various epigenetic changes, including differential expression of non-coding RNAs, changes in DNA methylation, and histone modifications in plants. Chapters detail protocols with different...
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Humana Press, 2017. — 403 p. — (Methods in Molecular Biology 1510). This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro. The book also covers the generation and specificity of deacetylase inhibitors and how such agents can be...
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Humana Press, 2017. — 279 p. — (Methods in Molecular Biology 1499). This volume provides an overview of the field and practical hints for vaccinologists in academia and industry. Chapters provide protocols on self-replicating RNA vectors, non-replication mRNA vectors, adjuvantation and delivery, and preclinical and clinical development. Written in the highly successful Methods in...
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Humana Press, 2019. — 279 p. — (Methods in Molecular Biology 1878). This volume covers a wide variety of state of the art cancer-related methods and tools for data analysis and interpretation. Chapters were designed to attract a broad readership, ranging from active researchers in computational biology and bioinformatics developers, clinical oncologists, and anti-cancer drug...
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Humana Press, 2013. — 255 p. Series: Methods in Molecular Biology, № 1020 Recently, many ground-breaking steps have been made towards better understanding NO/cGMP/PKG pathways, its components, substrates, and their localization within a given cell. These advances were only possible due to the development of sophisticated new techniques in the field. In this volume expert...
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Humana Press, 2015. — 284 p. Series: Methods in Molecular Biology №1347. This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations...
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2nd Edition. - Springer, 2015. - 474 p. Series Methods in Molecular Biology, Vоlume 1215 Molecular Modeling of Proteins, Second Edition provides a theoretical background of various methods available and enables non-specialists to apply methods to their problems by including updated chapters and new material not covered in the first edition. This detailed volume opens by...
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New York: Humana Press, 2015. — 369 p. — (Methods in Molecular Biology 1290). This detailed volume focuses on best practices and conditions for maintaining the most commonly used salamander species in the laboratory. Salamanders in Regeneration Research: Methods and Protocols guides readers through experimental manipulations in vivo and in vitro, respectively. With methods on...
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N Y: Humana Press, 2014. — 288 p. Series: Methods in Molecular Biology, № 1159 Biomedical Literature Mining, discusses the multiple facets of modern biomedical literature mining and its many applications in genomics and systems biology. The volume is divided into three sections focusing on information retrieval, integrated text-mining approaches and domain-specific mining methods....
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Humana Press, 2014 — 3rd Ed. — 799p. — (Methods in Molecular Biology 1117) — ISBN: 978-1-62703-776-1 (eBook), 978-1-62703-775-4 (Hardcover). This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related...
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Humana Press, 2013. — 329 p. Series: Methods in Molecular Biology, № 971 Lymphomas are lymphoid malignancies derived from B or T lymphocytes, and their study has been and still is paradigmatic for many aspects of cancer research. This volume presents and discusses key methods that are used in lymphoma research, partly specific for lymphoma research but often adaptable to the study...
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Humana Press, 2015 — 386p. — (Methods in Molecular Biology 1314) — ISBN: 978-1-4939-2718-0 (eBook), 978-1-4939-2717-3 (Hardcover). This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional...
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Humana Press, 2015 — 509p. — (Methods in Molecular Biology 1312) — ISBN: 978-1-4939-2694-7 (eBook), 978-1-4939-2693-0 (Hardcover). This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful Methods in...
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Humana Press, 2018. — 283 p. — (Methods in Molecular Biology). — ISBN 10 1493987445, 13 978-1493987443. This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant...
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Humana Press, 2002. — 288 p. Series: Methods in Molecular Biology, №212 Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography...
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Humana Press, 2014 — 555p. — (Methods in Molecular Biology 1129) — ISBN: 978-1-62703-977-2 (eBook), 978-1-62703-976-5 (Hardcover). Proteins are the most diverse group of biologically important substances. With the recent technological advances in the genomics area and the efforts in proteomics research, the rate of discovery for new proteins with unknown structure and function...
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Humana Press, 2018. - 301 p. - Volume 1686 of Methods in Molecular Biology. This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative...
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Humana Press, 2013. — 303 p. Series: Methods in Molecular Biology, № 997 Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement. While hESC are derived from a...
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Third Edition, Humana Press, 2012. — 359 p. — ISBN: 1627030859 Series: Methods in Molecular Biology, №937 The regulation of intracellular Ca 2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued. With the third edition, expert...
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2nd ed. — Humana Press, 2006. — 225 p. — ISBN 1-59259-949-4 Series: Methods in Molecular Biology, № 312 Contents Fluorescent Measurement of [Ca 2+ ]c: Basic Practical Considerations Measurement of [Ca 2+ ]i in Whole Cell Suspensions Using Fura-2 Measurement of [Ca 2+ ]i in Cell Suspensions Using Indo-1 Confocal Microscopy: Theory and Applications for Cellular Signaling Single-Cell...
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Humana Press, 2017. — 275 p. — (Methods in Molecular Biology 1503). This volume discusses detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides. The book covers topics such as the importance of glycans in eukaryotic life (with a focus on mammals and particularly humans); recent technologies that allow the characterization of larger...
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Humana Press, 2014. — 589 p. Series: Methods in Molecular Biology, № 1139 The book explores the manipulation and modification of immune cells, the manipulation and modification of tumor cells as well as the manipulation of immune/tumor interactions and various delivery mechanisms, with the overall end goal of evoking a tumor-specific response and overcoming the immuno-evasive...
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Humana Press, 2016 — 290p. — (Methods in Molecular Biology 1431) — ISBN: 978-1-4939-3631-1 (eBook), 978-1-4939-3629-8 (Hardcover). This volume details a valuable collection of protocols and reviews, such as emerging experimental and theoretical approaches. These approaches have resulted in a substantial improvement in the understanding of chromosome architecture. Chromosome...
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4th edition. — Humana Press, 2015. — 347 p. — (Methods in Molecular Biology 1334). This fourth edition reflects the most recent technical advances in DNA-protein interaction research. With fully updated chapters that describe techniques proven by their continuous value, the volume also many new chapters have been added that mostly deal with larger-scale experiments, reflecting...
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Humana Press, 2017. — 271 p. — (Methods in Molecular Biology 1605). — ISBN 10 1493969862. — ISBN 13 978-1493969869. This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and...
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Humana Press, 2015 — 2nd Ed. — 414p. — (Methods in Molecular Biology 1315) — ISBN: 978-1-4939-2715-9 (eBook), 978-1-4939-2714-2 (Hardcover). The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the...
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Humana Press, 2007. — 585 p. Series: Methods in Molecular Biology vol.372 Among life scientists, the mitochondrial community originally formed a rather separate and isolated subgroup studying a peculiar organelle. The relevance of mitochondrial research was thought to be limited to the field of bioenergeties. This view changed considerably during the last 10 to 15 years when it...
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Humana Press, 2018. - 375 p. - Volume 1673 of Methods in Molecular Biology. This detailed volume provides scientists interested in quorum sensing with a broad spectrum of methods and protocols useful for studying bacterial communication processes at the chemico-physical, molecular, and physiological level. Divided into three sections, the content covers detection and...
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Humana Press, 2015 — 255p. — (Methods in Molecular Biology 1331) — ISBN: 978-1-4939-2874-3 (eBook), 978-1-4939-2873-6 (Hardcover). This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for...
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Humana Press, 2014. — 469 p. Series: Methods in Molecular Biology, № 1092 In this volume, expert researchers in the field detail many of the protocols used to study mouse embryology. These include protocols and techniques that are "close to the embryo": such as, manipulating embryonic gene expression, culturing explanted embryonic tissue and harvesting embryonic RNA. With...
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New York: Humana Press, 2015. — 358 p. — (Methods in Molecular Biology 1298). This volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The book consists of 28 chapters and starts with an overview of the Rab...
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Humana Press, 2001. — 378 p. Series: Methods in Molecular Biology, № 176 Research into steroid receptor biology holds great promise for the treatment of cancer and endocrine disorders and for elucidating the fundamental biological mechanisms of gene expression. In this volume a distinguished team of principal investigators and their associates describe in step-by-step detail a...
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Humana Press, 2004. — 350 p. — (Methods in Molecular Biology 241). In recent years, cell cycle checkpoints, cellular mechanisms that control the cell cycle and ensure genomic stability, have emerged as significant factors in carcinogenesis and in cancer cells. In Cell Cycle Checkpoint Control Protocols, leading investigators present their best methodologies to probe the mechanisms...
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Humana Press, 2005 — 513 p. — eISBN 1-59259-848-X Series: Methods in Molecular Biology, № 292 The application of modern methods in molecular biology and biotechnology to the study of human, animal, and plant viruses continues to revitalize the age-old discipline of virology. Modern virology remains at the vanguard of contemporary biomedical research largely owing to the impact of...
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Humana Press, 2017. — 295 p. — ISBN 1493969234. Series: Methods in Molecular Biology, № 1592 This volume provides a collection of methodologies for basic research, clinical diagnosis, and treatment pertaining to food allergens, including food allergen production, purification, characterization, detection, quantification, and bioinformatics approaches to modern food allergen...
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Humana Press, 2016 — 288p. — (Methods in Molecular Biology 1421) — ISBN: 978-1-4939-3591-8 (eBook), 978-1-4939-3589-5 (Hardcover). This detailed volume explores the continuing techniques of studying RNA-protein complexes and interactions as research in these areas expand. After an introductory chapter, the book continues with ways to purify RNA-protein complexes assembled in...
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Humana Press, 1998. — 320 p. — (Methods in Molecular Biology 98). In this volume leading forensic experts from around the world describe in detail their time-proven methods for identity testing through DNA analysis. Their state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The...
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Humana Press, 2017. — 4394 p. — (Methods in Molecular Biology 1508). This detailed volume presents timely and authoritative content offering a comprehensive overview of the current state of the art in fungal diagnostics. Moreover, it addresses on-going developments expected to provide a basis for targeted treatment strategies resulting in improved outcome of invasive mycoses. The...
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Humana Press, 2015 — 271p. — (Methods in Molecular Biology 1319) — ISBN: 978-1-4939-2748-7 (eBook), 978-1-4939-2747-0 (Hardcover). In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and...
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New York: Humana Press, 2014. — 292 p. — (Methods in Molecular Biology 1084). In this book, expert researchers in the field detail both experimental and computational methods to interrogate molecular level fluctuations. Chapters detail best-practice recipes covering both experimental and computational techniques, reflecting modern protein research. Written in the highly successful...
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Humana Press, 1992. - 373 p. ISBN 0896031993 Series: Methods in Molecular Biology №13 Contents Separation of Large DNA Molecules by Pulsed-Field Gel Electrophoresis. The Isolation of Genomic DNA from Invertebrates. Analysis of Mihondrial DNA Mutations. The Design and Use of Oligonucleotides. The Use of Degenerate Oligonucleotides for Polymerase Chain-Reaction-Based...
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Humana Press Inc., 2002. — 229 p Series: Methods in Molecular Biology, № 184 Contents Dedication Preface Contributors Statistical Contributions to Molecular Biology Linking Image Quantitation and Data Analysis Introduction to Microarray Experimentation and Analysis Statistical Methods for Proteomics Statistical Methods for Assessing Biomarkers Power and Sample Size...
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New York: Humana Press, 2015. — 411 p. This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of...
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New York: Humana Press, 2015. — 261 p. This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types...
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Humana Press, 2017. — 272 p. — (Methods in Molecular Biology 1514). This volume provides protocols to successfully apply cutting-edge technologies to characterize the biology of T cells at an unprecedented level of complexity. Chapters guide readers through flow cytometry and fluorescence-activated cell sorting, the behaviour of single T cells after adoptive cell transfer (ACT),...
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Humana Press, 2005 — 337 p. — (Methods in Molecular Biology 307). — ISBN 1-59259-901-X. Study of Cyclic Adenosine Monophosphate Microdomains in Cells High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains Cygnets: In Vivo Characterization of Novel cGMP Indicators and In Vivo Imaging of Intracellular cGMP High-Throughput Screening of...
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Humana Press, 2015 — 366p. — (Methods in Molecular Biology 1311) — ISBN: 978-1-4939-2687-9 (eBook), 978-1-4939-2686-2 (Hardcover). This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques...
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