Ménard R. (Ed.) Malaria: Methods and Protocols

Second Edition, Humana Press, 2012. - 614 p. ISBN: 1627030255Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, 'omic' approaches, and techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Malaria: Methods and Protocols, Second Edition, offers a comprehensive set of standard techniques for laboratory researchers.Contents
Parasite Culturing
In vitro Culturing P. falciparum Erythrocytic Stages
Production of Plasmodium falciparum Gametocytes in vitro
In vitro Differentiation of Plasmodium falciparum Gametocytes into Ookinetes
Ex vivo culture of Plasmodium Vivax and Plasmodium Cynomolgi and in vitro Culture of Plasmodium Knowlesi Blood Stages
Laboratory Maintenance of Rodent Malaria Parasites
Genome Manipulation
Transfection of Plasmodium falciparum
Transfection of Rodent Malaria Parasites
Recombination-mediated Genetic Engineering of Plasmodium berghei DNA
Standardization in Generating and Reporting Genetically Modified Rodent Malaria Parasites: The RMgmDB Database
Transient Transfection of Plasmodium vivax Blood-stage Parasites
Silencing of Genes and Alleles by RNAi in Anopheles gambiae
‘OMIC’ Techniques
Analysis of Variant Gene Family Expression by Quantitative PCR
DNA Microarray-based Genome-wide Analyses of Plasmodium Parasites
Whole-genome Analysis of Plasmodium spp. Utilizing a New Agilent Technologies DNA Microarray Platform
Transcriptome Analysis using RNA-seq
Experimental Tools for the Study of Protein Phosphorylation in Plasmodium
Extraction of Hydrophilic Metabolites from Plasmodium falciparum-infected Erythrocytes for Metabolomic Analysis
Red Blood Cell Stages
Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in Plasmodium falciparum
Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Plasmodium falciparum Merozoites
Microsphiltration: a Microsphere Matrix to Explore Erythrocyte Deformability
Atomic Force Microscopy of Plasmodium-infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition Events
Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System
Genome-wide Chromatin Immunoprecipitation-sequencing in Plasmodium
In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparum
Bioluminescence Imaging of P. berghei Schizont Sequestration in Rodents
Pre-Erythrocytic Stages
Scoring Sporozoite Motility
Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry
Imaging Sporozoite Cell Traversal in the Liver of Mice
Analysis of Liver Stage Development in, and Merozoite Release from Hepatocytes
Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence Imaging
Immunity to Plasmodium
Activation of Human NK Cells by Plasmodium-infected Red Blood Cells
Chimeric Parasites as Tools to Study Plasmodium Immunology and Assess Malaria Vaccines
Development and Use of TCR Transgenic Mice for Malaria Immunology Research
Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination
Controlling Plasmodium Infection
Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter Parasites
Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using Cytometry
Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug Cure
Vaccination using Radiation-or Genetically-Attenuated Live Sporozoites
Vaccination using Normal Live Sporozoites Under Drug Treatment
Assessing Transmission Blockade in Plasmodium spp.
Mosquito Transgenic Technologies to Reduce Plasmodium Transmission