Lambert D., Rainbow R. (Ed.) Calcium Signaling Protocols

Third Edition, Humana Press, 2012. — 359 p. — ISBN: 1627030859Series: Methods in Molecular Biology, №937The regulation of intracellular Ca²⁺ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued. With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca²⁺ regulation and provided much new information on processes. Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca²⁺ signalling for screening, and genetically encoded sensors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca²⁺.General
Fluorescent Measurement of [Ca²⁺]c: Basic Practical Considerations
Measurement of [Ca²⁺]i in Whole Cell Suspensions Using Fura-2
Measurement of [Ca²⁺]i in Cell Suspensions Using Indo-1
Specialist Measurement Systems
Confocal Microscopy: Theory and Applications for Cellular Signaling
Single-Cell and Subcellular Measurement of Intracellular Ca²⁺ Concentration
Ratiometric Ca²⁺ Measurements Using the FlexStation Scanning Fluorometer
Measuring Ca²⁺ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader
Measurement of Ca²⁺ Channel Activity
Measurement of Ca²⁺ Entry Using 45Ca²⁺
Measurement of [³H]PN200-110 and [¹²⁵I]ω-Conotoxin MVIIA Binding
Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca²⁺ Channel Currents: Heterologous Expression Systems and Dissociated Brain Neurons
Measurement of INS(1,4,5)P3 and Ca²⁺ Release from Intracellular Stores
Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [³H]Inositol-Labeling Protocols in Permeabilized Cells
Measurement of Inositol(1,4,5)Triphosphate Using a Stereospecific Radioreceptor Mass Assay
Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca²⁺ Uptake and Release Assay
Microinjection of myo-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents Into Intact Adherent Cells
Measurement of Free [Ca²⁺] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators
Specialist Measurement Techniques
Measurement of [Ca²⁺]i in Smooth Muscle Strips Using Front-Surface Fluorimetry
Measurement of Calcium and Movement in Heart Cells
Simultaneous Analysis of Intracellular pH and Ca²⁺ From Cell Populations
Measurement of Cytosolic-Free Ca²⁺ in Plant Tissue
Ca²⁺-Sensitive Targets
Assay and Purification of Calmodulin-Dependent Protein Kinase
Assay and Purification of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase and Isozyme Separation
Measurement of Ca²⁺-ATPase Activity (in PMCA and SERCA1)