Dickinson M., Hodgetts J. (Ed.) Phytoplasma: Methods and Protocols

Humana Press, 2012. - 419 p. ISBN: 1627030883
Series: Methods in Molecular Biology, №938Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply. Phytoplasmas can cause serious and devastating problems for crop plants, especially in developing countries. In Phytoplasmas: Methods and Protocols experts in the field cover topics pertinent to scientists intending to develop a research program on phytoplasmas. The volume opens with an introductory chapter on the importance of phytoplasma diseases, followed by methods for detection and diagnosis as well as techniques for separating and classifying the phytoplasmas into their different taxonomic groups and subgroups. The final chapters cover methods for separating phytoplasma genomic and plasmid DNA from plant DNA for whole genome sequencing. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Phytoplasmas: Methods and Protocols serves as an ideal guide to plant pathologists and molecular biologists aiming to set up a diagnostics facility for identifying the presence of these pathogens in plants or their insect vectors.The Phytoplasmas: An Introduction
Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus
Micropropagation and Maintenance of Phytoplasmas in Tissue
Dodder Transmission of Phytoplasmas
Insect Maintenance and Transmission
Capturing Insect Vectors of Phytoplasmas
Insect Vector Transmission Assays
Molecular Identification of Phytoplasma Vector Species
Dienes’ Staining and Light Microscopy for Phytoplasma
DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas
Visualization of Phytoplasmas Using Electron Microscopy
Automated DNA Extraction for Large Numbers of Plant Samples
DNA Extraction from Arborescent Monocots and How to Deal with Other Challenging
Nested PCR and RFLP Analysis Based on the 16S rRNA Gene
PCR and RFLP Analyses Based on the Ribosomal Protein Operon
Tuf and secY PCR Amplification and Genotyping of
PCR Analysis of Phytoplasmas Based on the secA Gene
Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma
Microarrays for Universal Detection and Identification of Phytoplasmas
T-RFLP for Detection and Identification of Phytoplasmas in Plants
Real-Time PCR for Universal Phytoplasma Detection and Quantification
A Real-Time PCR Detection System for the Bois Noir and Flavescence Dorée Phytoplasmas and Quantification of the Target
Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation
Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions
In-Field Diagnostics Using Loop-Mediated Isothermal Amplification
DNA Bar-Coding for Phytoplasma Identification
Phylogenetic Analyses of Phytoplasmas Based on Whole-Genome Comparison.
The i PhyClassifier, an Interactive Online Tool for Phytoplasma Classification and Taxonomic Assignment
Phytoplasma Proteomic Analysis
Preparation of Phytoplasma Membrane Recombinant Proteins
Phytoplasma Plasmid DNA
Cesium Chloride-Bisbenzimide Gradients for Separation of Phytoplasma and Plant DNA
Pulsed-Field Gel Electrophoresis for Isolation of Full-Length Phytoplasma Chromosomes from Plants
Mapping the Phytoplasma Chromosome