Selinsky B.S. (Ed.). Membrane Protein Protocols: Expression, Purification, and Characterization

Humana Press, 2003. — 386 p.
Series: Methods in Molecular Biology, №228A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocols contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.