Hawley T.S., Hawley R.G. (Eds.). Flow Cytometry Protocols

2nd Edition. - Humana Press, 2004. - 425 p.
Series: Methods in Molecular Biology, №263In addition to providing chapters on the theoretical and practical aspects of flow cytometry, this book contains protocols used specifically for immediate use in the diagnostic and experimental measurements enabled by modern flow cytometric instrumentation. Research protocols cover cell cycle analysis and regulation, detection of rare cells, solid tumor DNA techniques, ploidy analysis and the application of flow cytometric methods for measurements of leukemias, necrosis, apoptosis, heat shock proteins, liver and bladder cancer, disease monitoring, and intracellular measurements. Hematologic and immunologic applications are also described by researchers with a particular expertise in their use. A special section is included for biotechnologists using flow cytometry in the study of protein secretion, the monitoring of fermentation processes, monitoring bioreactors, cell-cell electroporation, quantitation electrofusion, and electrochemotherapy.Flow cytometry : an introduction
Multiparameter flow cytometry of bacteria
Multiparameter data acquisition and analysis of leukocytes
Flow cytometric analysis of kinase signaling cascades
Cytokine flow cytometry
Use of cell-tracking dyes to determine proliferation precursor frequencies of antigen-specific T cells
Assessment of lymphocyte-mediated cytotoxicity using flow cytometry
Multiparametric analysis of apoptosis by flow and image cytometry
Detection and enrichment of haematopoietic stem cells by side population phenotype
Hematopoietic stem cell characterization by Hoechst 33342 and Rhodamine 123 staining
Phenotypic and functional analyses of CD34[superscript NEG] haematopoietic precursors from mobilized peripheral blood
Multiparameter flow cytometry of fluorescent protein reporters
Analysis of fluorescent protein expressing cells by flow cytometry
Integrative flow cytometric and microarray approaches for use in transcriptional profiling
Flow cytometric analysis of fluorescence resonance energy transfer : a tool for high-throughput screening of molecular interactions in living cells
Design of a fluorescence-activated cell sorting-based mammalian protein-protein interaction trap
Flow cytometric screening of yeast surface display libraries
Fluorescence resonance energy transfer-based HIV-1 virion fusion assay
Cell-cycle analysis of asynchronous populations
Solid tumor DNA content analysis
Concurrent flow cytometric analysis of DNA and RNA
Telomere length measurement by fluorescence in situ hybridization and flow cytometry
Small lasers in flow cytometry
Viable infections cell sorting in a BSL-3 facility