Turksen K. (Ed.). Embryonic Stem Cells: Methods and Protocols

Humana Press, 2002. - 516 p.
Series: Methods in Molecular Biology, №185The book could be used effectively by experienced or novice researchers in the field since the protocols are written clearly and concisely. There are 35 brief chapters in this book by over 80 authors who are experienced and knowledgeable researchers in the field.Among the many experimental protocols described in this book are methods for isolating, culturing, and establishing ES cells from a variety of experimental animals. The protocols are written clearly and should serve as a useful laboratory resource for anyone wishing to learn how to culture ES cells and/or apply the specialized techniques for studying them. There is an adequate index. This book is intended and written as a laboratory guide manual. It would be quite useful for anyone doing bench research on ES cells.Color Plates
Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells
The Use of Chemically Defined Media for the Analyses of Early Development in ES Cells and Mouse Embryos
Analysis of the Cell Cycle in Mouse Embryonic Stem Cells
Murine Embryonic Stem Cells as a Model for Stress Proteins and Apoptosis During Differentiation
Effects of Altered Gene Expression on ES Cell Differentiation
Hypoxic Gene Regulation in Differentiating ES Cells
Regulation of Gap Junction Protein (Connexin) Genes and Function in Differentiating ES Cells
Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development
Analysis of Bcr-Abl Function Using an In Vitro Embryonic Stem Cell Differentiation System
Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development
Differentiation of Embryonic Stem Cells as a Model to Study Gene Function During the Development of Adipose Cells
Embryonic Stem Cell Differentiation and the Vascular Lineage
Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation
Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: Strategies and Applications
Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System
Isolation of Lineage-Restricted Neural Precursors from Cultured ES Cells
Lineage Selection for Generation and Amplification of Neural Precursor Cells
Selective Neural Induction from ES Cells by Stromal Cell-Derived Inducing Activity and Its Potential Therapeutic Application in Parkinson's Disease
Epidermal Lineage
ES Cell Differentiation Into the Hair Follicle Lineage In Vitro
Embryonic Stem Cells as a Model for Studying Melanocyte Development
Using Progenitor Cells and Gene Chips to Define Genetic Pathways
ES Cell-Mediated Conditional Transgenesis
Switching on Lineage Tracers Using Site-Specific Recombination
From ES Cells to Mice: The Gene Trap Approach
Functional Genomics by Gene-Trapping in Embryonic Stem Cells
Phage-Displayed Antibodies to Detect Cell Markers
Gene Transfer Using Targeted Filamentous Bacteriophage
Single-Cell PCR Methods for Studying Stem Cells and Progenitors
Nonradioactive Labeling and Detection of mRNAs Hybridized onto Nucleic Acid cDNA Arrays
Expression Profiling Using Quantitative Hybridization on Macroarrays
Isolation of Antigen-Specific Intracellular Antibody Fragments as Single Chain Fv for Use in Mammalian Cells
Detection and Visualization of Protein Interactions with Protein Fragment Complementation Assays
Direct Selection of cDNAs by Phage Display
Screening for Protein–Protein Interactions in the Yeast Two-Hybrid System in Embryonic Stem Cells