Humphrey T., Brooks G. (Eds.). Cell Cycle Control: Mechanisms and Protocols

Humana Press, 2004. - 409 p.
Series: Methods in Molecular Biology, №296This collection of cutting–edge techniques for the study of the eukaryotic cell cycle and its key regulatory molecules includes overviews of cell cycle regulatory mechanisms in many major research organisms. Described in step-by-step detail, these readily reproducible methods enable fundamental research on well-defined cell cycle regulators—and those more recently defined—in yeasts, bacteria, plants, Drosophila, Xenopus, and mammals. The book offers all cell researchers indispensable techniques essential to understanding how normal cells divide and how this is altered in disease.Overviews of Cell Cycle Control in Different Organisms
Cell Cycle Molecules and Mechanisms of the Budding and Fission Yeasts
The Plant Cell Cycle: An Overview
The C. elegans Cell Cycle: Overview of Molecules and Mechanisms
Developmental Control of Growth and Cell Cycle Progression in Drosophila
The Xenopus Cell Cycle: An Overview
The Mammalian Cell Cycle: An Overvew
Cell Cycle Study Methods and Techniques
Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle
Mapping Origins of DNA Replication in Eukaryotes
In Situ Assay for Analyzing the Chromatin Binding of Proteins in Fission Yeast
Application of Magnetic Beads to Purify Cells Transiently Transfected With Plasmids Encoding Short Hairpin RNAs
The Cell Cycle and Virus Infection
Methods for Preparation of Proteins and Protein Complexes That Regulate the Eukaryotic Cell Cycle for Structural Studies
Detection and Measurement of Cell Cycle Molecules
E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression
Forkhead (FOX) Transcription Factors and the Cell Cycle: Measurement of DNA Binding by FoxO and FoxM Transcription Factors
Measurement of Geminin Activity in Xenopus Egg Extracts
CDK-Activating Kinases: Detection and Activity Measurements
Cyclins, Cyclin-Dependent Kinases, and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Activity Measurements
Measurement of Wee Kinase Activity
CDC25 Dual-Specificity Protein Phosphatases: Detection and Activity Measurements
Assaying Cell Cycle Checkpoints: Activity of the Protein Kinase Chk1
Polo-Like Kinase-1: Activity Measurement and RNAi-Mediated Knockdown
The Ipl1/Aurora Kinase Family: Methods of Inhibition and Functional Analysis in Mammalian Cells
Purification of the Ndc80 Kinehore Subcomplex From Xenopus Eggs