Robinson A.S. (Ed.). Production of Membrane Proteins

Wiley, 2011. - 443 p. - Designed as a research-level guide to current strategies and methods of membrane protein production on the small to intermediate scale, this practice-oriented book provides detailed, step-by-step laboratory protocols as well as an explanation of the principles behind each method, together with a discussion of its relative advantages and disadvantages.
Following an introductory section on current challenges in membrane protein production, the book goes on to look at expression systems, emerging methods and approaches, and protein specific considerations. Case studies illustrate how to select or sample the optimal production system for any desired membrane protein, saving both time and money on the laboratory as well as the technical production scale. Unique in its coverage of "difficult" proteins with large membrane-embedded domains, proteins from extremophiles, peripheral membrane proteins, and protein fragments.Expression systems
Bacterial systems
Membrane protein expression in Saccharomyces cerevisiae
Expression systems: Pichia pastoris
Heterologous production of active mammalian g-protein-coupled receptors using Baculovirus-infected insect cells
Membrane protein expression in mammalian cells
Membrane protein producting using photosynthetic bacteria: a practical guide
Protein-specific considerations
Peripheral membrane protein production for structural and functional studies
Expression of g-protein-coupled receptors
Structural biology of membrane proteins
Emerging methods and approaches
Engineering integral membrane proteins for expression and stability
Expression and purification of g-protein-coupled receptors for nuclear magnetic resonance structural studies
Solubilization, purification, and characterization of integral membrane proteins
IMPs stabilizing membrane proteins in detergent and lipid systems
Rapid optimization of membrane protein production using green fluorescent protein-fusions and LEMO21(DE3)