Wu С.J. Protein Microarray for Disease Analysis: Methods and Protocols (Methods in Molecular Biology)

Humana Press; 1st Edition. edition (March 3, 2011) 386 pages ISBN-10: 1617790427Protein microarrays have been used for a wide variety of important tasks, such as identifying protein-protein interactions, discovering disease biomarkers, identifying DNA-binding specificity by protein variants, and for characterization of the humoral immune response. In Protein Microarray for Disease Analysis: Methods and Protocols, expert researchers provide concise descriptions of the methodologies currently used to fabricate microarrays for the comprehensive analysis of proteins or responses to proteins that can be used to dissect human disease. These methodologies are the toolbox for revolutionizing drug development and cell-level biochemical understanding of human disease processes. Beginning with a section on protein-detecting analytical microarrays, the volume continues with sections covering antigen microarrays for immunoprofiling, protein function microarrays, the validation of candidate targets, proteomic libraries, as well as signal detection strategies and data analysis techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Protein Microarray for Disease Analysis: Methods and Protocols serves as a solid framework to aid scientists in understanding how protein microarray technology is presently developing and how it can be applied to transform our analysis of human disease.Contents
Protein-Detecting Analytical Microarrays
Detecting and Quantifying Multiple Proteins in Clinical Samples in High-Throughput Using Antibody Microarrays
Analysis of Serum Protein Glycosylation with Antibody-Lectin Microarray for High-Throughput Biomarker Screening
Antibody Suspension Bead Arrays
Reverse Protein Arrays Applied to Host-Pathogen Interaction Studies
Identification and Optimization of DNA Aptamer Binding Regions Using DNA Microarrays
Recombinant Lectin Microarrays for Glycomic Analysis
Antigen Microarrays for Immunoprofiling
Recombinant Antigen Microarrays for Serum/Plasma Antibody Detection
SPOT Synthesis as a Tool to Study Protein-Protein Interactions
Native Antigen Fractionation Protein Microarrays for Biomarker Discovery
Immunoprofiling Using NAPPA Protein Microarrays
Protein Function Microarrays
High-Throughput Mammalian Two-Hybrid Screening for Protein-Protein Interactions Using Transfected Cell Arrays (CAPPIA)
Protein-Protein Interactions: An Application of Tus-Ter Mediated Protein Microarray System
Kinase Substrate Interactions
A Functional Protein Microarray Approach to Characterizing Posttranslational Modifications on Lysine Residues
Strategies for Validation of Candidate Targets
Multiplexed Detection of Antibodies Using Programmable Bead Arrays
A Co-Precipitation-Based Validation Methodology for Interactions Identified Using Protein Microarrays
Generation of Proteomic Libraries
Development of Expression-Ready Constructs for Generation of Proteomic Libraries
Detection Methods
Reverse Phase Protein Microarrays: Fluorometric and Colorimetric Detection
Förster Resonance Energy Transfer Methods for Quantification of Protein-Protein Interactions on Microarrays
Label-Free Detection with Surface Plasmon Resonance Imaging
Data Analysis Techniques for Protein Function Microarrays
Data Processing and Analysis for Protein Microarrays
Database Resources for Proteomics-Based Analysis of Cancer