Davies Gwyn (Ed.). Vaccine Adjuvants: Methods and Protocols

Humana Press, 2010. — 324 p. — (Methods in Molecular Biology, vol.626). — ISBN-10: 1607615843, ISBN-13: 978-1607615842.Product DescriptionSpanning from discoveries in fundamental immunology to industrial and commercial concerns, the study of vaccine adjuvants has developed into an exciting area of work with great, vital potential in innovating techniques in which adjuvants may steer the immune system towards the responses required by unmet vaccination needs. In Vaccine Adjuvants: Methods and Protocols, expert researchers in the field provide clear and concise guidance on how to go about assessing the activity of adjuvant products. Rather than describing individual adjuvants, the volume strives to include detailed, practical information on measuring the responses produced by adjuvants in order to be relevant to the widest array of experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and versatile, Vaccine Adjuvants: Methods and Protocols will enable those already pursuing vaccine adjuvant research, while also serving to stimulate discussion on how to best standardize adjuvant testing in order to facilitate meaningful comparisons, and above all, to aid in the prediction of which new products will most effectively and safely help to solve the current challenges in vaccination.Immunology of Vaccine Adjuvants
Preclinical Development of AS04
Nonclinical Safety Assessment of Vaccines and Adjuvants
Aluminum Adjuvants: Preparation, Application, Dosage, and Formulation with Antigen
Freund’s Complete and Incomplete Adjuvants, Preparation, and Quality Control Standards for Experimental Laboratory Animals Use
Liposomal Adjuvants: Preparation and Formulation with Antigens
Micro/Nanoparticle Adjuvants: Preparation and Formulation with Antigens
Adjuvant Activity on Human Cells In Vitro
Adjuvant Activity on Murine and Human Macrophages
In Vitro Effects of Adjuvants on B Cells
NKT Cell Responses to Glycolipid Activation
Tracking Dendritic Cells In Vivo
Adjuvant Effects on Antibody Titre
Functional Antibody Assays
Determining Adjuvant Activity on T-Cell Function In Vivo: Th Cells
Quantitative Multiparameter Assays to Measure the Effect of Adjuvants on Human Antigen-Specific CD8 T-Cell Responses
Large-Animal Model for Establishing E/T Ratio of Adjuvants
Determining the Activity of Mucosal Adjuvants
Adjuvant Activity of Cytokines
Subject Index