Fields G.B. Peptide Characterization and Application Protocols

Humana, 2007. — 244 p.Peptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical systems and the contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques, including high-performance liquid chromatography (HPLC) for purification and evaluation of peptides and mass spectrometry (MS) for the analysis of standard and modified peptides. Innovative methods of synthesis and characterization of membrane peptides are also presented. Part II, Application, presents a variety of specific applications for synthetic peptides. The collected protocols demonstrate the systematic manner in which peptides can be constructed, analyzed, and applied to attack contemporary problems in biochemistry, and this book will be an indispensable aid in the pursuit of new directions in peptide research.Preface
Contributors
Characterization
HPLC Analysis and Purification of Peptides
Identification of Proteins Based on MS/MS Spectra and Location of Posttranslational Modifications
Determination of Phosphorylated and O-Glycosylated Sites by Chemical Targeting (CTID) at Ambient Temperature
Synthesis, Biosynthesis, and Characterization of Transmembrane Domains of a G Protein--Coupled Receptor
Applications
Application of Topologically Constrained Mini-Proteins as Ligands, Substrates, and Inhibitors
Proteolytic Profiling of the Extracellular Matrix Degradome
-Amyloid Protein Aggregation
Radiometal-Labeled Somatostatin Analogs for Applications in Cancer Imaging and Therapy
Cell-Penetrating Proline-Rich Peptidomimetics
Peptide-Mediated Targeting of Liposomesto Tumor Cells
Peptide-Mediated Delivery of Nucleic Acids into Mammalian Cells
Broth Microdilution Antibacterial Assay of Peptides
Depsipeptide Synthesis
Index