Chaohong Liu (ed.) Invariant Natural Killer T-Cells: Methods and Protocols

Humana, 2021. — 199 p.This detailed book focuses on various aspects of Invariant Natural Killer T-Cells (iNKT), which are known to contribute to homeostasis and autoimmunity and can also cause various pathological responses such as allergy, infection, excessive autoimmune response, and cancer. The volume explores techniques for identification and isolation of iNKT cells, iNKT cell activation and transformation, as well as proliferation and differentiation and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Invariant Natural Killer T-Cells: Methods and Protocols is an ideal guide for researchers working with and studying iNKT cells, perhaps to provide a deeper knowledge of the human immune system.Preface
Contributors
Genetic Analysis of iNKT Cell Development and Function
Genetic Studies of Natural Glycosphingolipid Ligands for NKT Cells
Retroviral Transduction of NKT Hybridoma Cells
Methods for Studying Mouse and Human Invariant Natural Killer T Cells
Isolation and Detection of Murine iNKT Cells in Different Organs
Identifying, Isolation, and Functional Use of Human Liver iNKT Cells
Isolation and Characterization Methods of Human Invariant NKT Cells
Detection of Mouse Type I NKT (iNKT) Cells by Flow Cytometry
Identification of Rare Thymic NKT Cell Precursors by Multiparameter Flow Cytometry
iNKT Cel Transfer: The Use of Cell Sorting Combined with Flow Cytometry Validation Approach
Expansion of Human iNKT Cells Ex Vivo
The Expansion and Cytotoxicity Detection of Human iNKT Cells
Ex Vivo Expansion of Th2-Polarizing Immunotherapeutic iNKT Cells from Human Peripheral Blood
Intravital Microscopy Imaging of Invariant Natural Killer T-Cell Dynamics in the Liver Using CXCR6-eGFP Transgenic...
In Vivo Cytotoxicity by α-GalCer-transactivated NK Cells
Redirecting iNKT Cell Antitumor Immunity with α-GalCer/CD1d-scFv Fusion Proteins
Investigating the Dynamic Changes in iNKT Cell Metabolic Profiles During Development
Index