Solanas G., Welz P.-S. (eds.) Circadian Regulation: Methods and Protocols

Humana, 2022. — 413 p.This volume details methods on several aspects of circadian research. Chapters guide readers through the latest techniques and a wide variety of daily rhythmic processes, model organisms, circadian rhythms in the SCN and in peripheral organs, and describing in vitro systems and in silico methods. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Circadian Regulation: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
Chapters 3, 4, 8, and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.Preface
Contributors
Sleep Under Preindustrial Conditions: What We Can Learn from It
The Structure-Based Molecular-Docking Screen Against Core Clock Proteins to Identify Small Molecules to Modulate th...
Analysis of Complex Circadian Time Series Data Using Wavelets
Mathematical Modeling in Circadian Rhythmicity
Bioinformatics and Systems Biology of Circadian Rhythms: BIO_CYCLE and CircadiOmics
Cell-Based Phenotypic Screens to Discover Circadian Clock-Modulating Compounds
Methods for Assessing Circadian Rhythms and Cell Cycle in Intestinal Enteroids
Using ALLIGATORs to Capture Circadian Bioluminescence
Studying Circadian Clock Entrainment by Hormonal Signals
In Vitro Assays for Measuring Intercellular Coupling Among Peripheral Circadian Oscillators
Circadian Control of Transcriptional and Metabolic Rhythms in Primary Hepatocytes
Electrophysiology of the Suprachiasmatic Nucleus: Single-Unit Recording
Anatomical Methods to Study the Suprachiasmatic Nucleus
Circadian Analysis of Rodent Locomotor Activity in Home Cages
Recording of Diurnal Gene Expression in Peripheral Organs of Mice Using the RT-Biolumicorder
Isolation and Sorting of Epidermal Interfollicular Stem Cells for the Study of Circadian Rhythms
Detecting Circadian Rhythms in Human Red Blood Cells by Dielectrophoresis
Measuring Circadian Neutrophil Infiltration in Tissues by Paired Whole-Mount Tissue Clearing and Flow Cytometry
In Vivo Imaging of Circadian NET Formation During Lung Injury by Four-Dimensional Intravital Microscopy
Real-Time Measurement of Energy Metabolism Over Circadian Time Using Indirect Calorimetry-Enabled Metabolic Cages
Untargeted and Targeted Circadian Metabolomics Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) and...
Time-Restricted Feeding and Caloric Restriction: Two Feeding Regimens at the Crossroad of Metabolic and Circadian...
Chromatin Immunoprecipitation and Circadian Rhythms
Fluorescent Reporters for Studying Circadian Rhythms in Drosophila melanogaster
Visualization of Mutant Aggregates from Clock Neurons by Agarose Gel Electrophoresis (AGERA) in Drosophila melanog...
Methods for Delivery of dsRNAi Against Canonical Clock Genes and Immunocytodetection of Clock Proteins in Crustacea
In Vivo Bioluminescence Analyses of Circadian Rhythms in Arabidopsis thaliana Using a Microplate Luminometer
Index