Hee-Jeon Hong (ed.) Bacterial Cell Wall Homeostasis: Methods and Protocols

Humana, 2016. — 286 p.This volume brings together the most widely used and important protocols currently being employed in researching and understanding bacterial cell wall homeostasis. Chapters in Bacterial Cell Wall Homeostasis cover a variety of subjects, such as: modern microscopy techniques and other biophysical methods used to characterize the subcellular structure of the bacterial cell wall; high-throughput approaches that can be used to identify all the genes and proteins that participate in the correct functioning of an organism’s cell wall; protocols for assaying individual gene products for specific cell wall functions or identify chemicals with inhibitory activity against the cell wall; and methods for analyzing the non-protein components of the cell wall and the increasing use of computational approaches for predicting and modeling cell wall related functions and processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Bacterial Cell Wall Homeostasis: Methods and Protocols emphasizes the diversity of the research taking place in bacterial cell wall homeostasis, and explains how the integration of information from across multiple disciplines is going to be essential if a holistic understanding of this important process is to be obtained.Preface
Contributors
Analyzing Physical Properties of the Bacterial Cell Wall
Atomic Force Microscopy Analysis of Bacterial Cell Wall Peptidoglycan Architecture
Ultra-Sensitive, High-Resolution Liquid Chromatography Methods for the High-Throughput Quantitative Analysis of Bacterial Cell Wall Chemistry and Structure
Genome-Wide Approaches for the Identification of Gene Products with Roles in Cell Wall Homeostasis
Microarray Analysis to Monitor Bacterial Cell Wall Homeostasis
Cell Shaving and False-Positive Control Strategies Coupled to Novel Statistical Tools to Profile Gram-Positive Bacterial Surface Proteomes
Differential Proteomics Based on Multidimensional Protein Identification Technology to Understand the Biogenesis of Outer Membrane of Escherichia coli
Random Transposon Mutagenesis for Cell-Envelope Resistant to Phage Infection
Functional Analysis of Cell-Wall Associated Proteins
Zymographic Techniques for the Analysis of Bacterial Cell Wall in Bacillus
Liquid Chromatography-Tandem Mass Spectrometry to Define Sortase Cleavage Products
Genetics and Cell Morphology Analyses of the Actinomyces oris srtA Mutant
Reporter Assays for Cell Wall Stress
Construction of a Bioassay System to Identify Extracellular Agents Targeting Bacterial Cell Envelope
Luciferase Reporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus
Analysis of the Non-Protein Components of the Cell Wall
Extraction and Analysis of Peptidoglycan Cell Wall Precursors
Continuous Fluorescence Assay for Peptidoglycan Glycosyltransferases
Analysis of Peptidoglycan Fragment Release
Analysis of Cell Wall Teichoic Acids in Staphylococcus aureus
Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy
Bioinformatics and Computational Biology Based Approaches
Biophysical Measurements of Bacterial Cell Shape
Coarse-Grained Molecular Dynamics Simulations of the Bacterial Cell Wall
Structural Comparison and Simulation of Pneumococcal Peptidoglycan Hydrolase LytB
Index