Zernicka-Goetz M., Turksen K. (eds.) Embryo Models In Vitro: Methods and Protocols

Humana, 2024. — 327 p.This collection explores the use of pluripotent cells to generate early embryo-like structures in vitro, with the resultant 3D embryo-like structures in vitro phenocopying many of the developmental landmarks and 3D architectures seen normally in vivo. The protocols gathered herein cover diverse aspects of the topic and exemplify some of the key developments and improvements in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Embryo Models In Vitro: Methods and Protocols serves as an ideal guide to these exciting and innovative advances in developmental biology.Preface
Contributors
Generation of Human Blastoids from Naive Pluripotent Stem Cells
Human Pre-gastrulation Embryo Culture in 3D Condition
Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System
Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models
Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology
Endometrial Assembloids to Model Human Embryo Implantation In Vitro
Stem Cell-Derived Microfluidic Amniotic Sac Embryoid (μPASE)
Generation of 3D Trophoblast Organoids from Human Naïve Pluripotent Stem Cells
Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells
Modeling Human Paraxial Mesoderm Development with Pluripotent Stem Cells
Generation of Stem Cell-Based Mouse Embryo-Like Structures
Embryonic Spinal Cord Innervation in Human Trunk Organogenesis Gastruloids: Cardiac Versus Enteric Customization and Beyond
Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts
Directed Differentiation of Human Pluripotent Stem Cells to Cytotrophoblast and Syncytiotrophoblast
Single-Cell mRNA-sncRNA Co-sequencing of Preimplantation Embryos
Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome Atlas
Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL
ShapeMetrics: A 3D Cell Segmentation Pipeline for Single-Cell Spatial Morphometric Analysis
Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence
Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell Genomics
Whole-Mount RNA, Single-Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos
Index