Kriechbaumer Verena (ed.) The Plant Endoplasmic Reticulum: Methods and Protocols

2nd edition. — Humana Press, 2024 — 434 p. — (Methods in Molecular Biology 2772). — ISBN 978-1-0716-3710-4.This second edition provides new and updated methods detailing techniques and state-of-the-art approaches on the structure and function of plant endoplasmic reticulum (ER). Chapters guide readers through modern microscopy techniques, software protocols, purification, and analysis of ER membrane structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, The Plant Endoplasmic Reticulum: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.Make It Shine: Labelling the ER for Light and Fluorescence Microscopy
Electron Microscopy Techniques for 3D Plant ER Imaging
Studying Plant ER-PM Contact Site Localized Proteins Using Microscopy
Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques
Quantitation of ER Morphology and Dynamics
Long-Term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination
Dancing with the Stars: Using Image Analysis to Study the Choreography of the Endoplasmic Reticulum and Its Partners and of Movement Within Its Tubules
Preparation of Highly Enriched ER Membranes Using Free-Flow Electrophoresis
Preparation of ER Microsomes from Arabidopsis thaliana
ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis
2 in 1 Vectors Improve in Planta BiFC and FRET Analysis
Immunoprecipitation and FRET-FLIM to Determine Metabolons on the Plant ER
Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells
Protein Biosynthesis and Maturation in the ER
ER Membrane Protein Interactions Using the Split-Ubiquitin System (SUS)
Analysis of Protein Glycosylation in the ER
Unfolded Protein Response in Arabidopsis
Imaging the ER and Endomembrane System in Cereal Endosperm
Multi-omics Resources for Understanding Gene Regulation in Response to ER Stress in Plants
Variable-Angle Epifluorescence Microscopy for Single-Particle Tracking in the Plant ER
Use of Super-Resolution Live Cell Imaging to Distinguish Endoplasmic Reticulum: Nuclear Envelope Subcellular Localization
Using ER-Targeted Photoconvertible Fluorescent Proteins in Living Plant Cells
In Vivo Analysis of ER-Associated Protein Degradation and Ubiquitination in Arabidopsis thaliana
Overexpression of Endoplasmic Reticulum Proteins from Arabidopsis thaliana in Baculovirus
Observing ER Dynamics over Long Timescales Using Light Sheet Fluorescence Microscopy
Gene Stacking and Stoichiometric Expression of ER-Targeted Constructs Using “2A” Self-Cleaving Peptides
Automated Segmentation and 3D Reconstruction of Different Membranes from Confocal Z-Stacks
Visualizing Orientation and Topology of ER Membrane Proteins In Planta
Organ-Specific Microsomes from Dark-Grown Hypocotyls of Arabidopsis thaliana
Studying Secretory Protein Synthesis in Nicotiana benthamiana Protoplasts
Analyzing Photoactivation with Diffusion Models to Study Transport in the Endoplasmic Reticulum Network