Azeredo Joana, Sillankorva Sanna (eds.) Bacteriophage Therapy: From Lab to Clinical Practice

2nd edition. — Humana Press, 2024. — 316 p. — (Methods in Molecular Biology 2734). — ISBN 978-1-0716-3522-3.This highly anticipated second edition details cutting-edge experimental approaches for bacteriophage research. This comprehensive volume details new and updated experimental approaches for isolating, formulating, and engineering bacteriophage medicinal products. Authored by leading experts and written in the highly successful Methods in Molecular Biology series format, each chapter includes introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and valuable tips for troubleshooting and avoiding known pitfalls.Whether you are a seasoned scientist or a newcomer, Bacteriophage Therapy: From Lab to Clinical Practice, Second Edition provides essential tools and knowledge to advance bacteriophage research and its translation into innovative medicinal products and clinical therapies.Isolation, and Characterization of Bacteriophages
Isolation of Bacteriophages for Clinically Relevant Bacteria
Observation of Bacteriophage Ultrastructure by Cryo-Electron Microscopy
Bacteriophage Taxonomy:​ A Continually Evolving Discipline
Selection, Production, and Encapsulation of Bacteriophages
Guidelines to Compose an Ideal Bacteriophage Cocktail
Rapid Bench to Bedside Therapeutic Bacteriophage Production
Bacteriophage Production in Compliance with Regulatory Requirements
Nano/​microformulation​s for Bacteriophage Delivery
From In Vitro to Animal Efficacy Studies
Phage-Host Interaction Analysis Using Flow Cytometry
Bacteriophage Control of Infectious Biofilms
Studying Bacteriophage Efficacy Using a Zebrafish Model
Use of Galleria mellonella as an Animal Model for Studying the Antimicrobial Activity of Bacteriophages with Potential Use in Phage Therapy
Host Interaction and Clinical Application
Interaction of Bacteriophages with the Immune System:​ Induction of Bacteriophage-Specific Antibodies
Bacteriophage Treatment of Infected Diabetic Foot Ulcers
A Review of Phage Therapy for Bone and Joint Infections
Successful Use of Phage and Antibiotics Therapy for the Eradication of Two Bacterial Pathogens from the Respiratory Tract of an Infant
Phage Engineering Approaches
Genetic Engineering and Rebooting of Bacteriophages in L-Form Bacteria
Synthetic Biology to Engineer Bacteriophage Genomes
Genetic Engineering of Therapeutic Phages Using Type III CRISPR-Cas Systems
Robust and Reproducible Protocol for Phage Genome “Rebooting” Using Transformation-Associated Recombination (TAR) Cloning into Yeast Centromeric Plasmid