Sullivan Millicent O., Chackerian Bryce, Chen Wilfred (eds.) Therapeutic Proteins: Methods and Protocols

Humana Press, 2024. — 248 p. — (Methods in Molecular Biology 2720). — ISBN 978-1-0716-3468-4.This volume covers the latest key aspects of therapeutic protein applications. Chapters in this book cover topics such as the discovery, production, and conjugation of protein-proteins with discussions on the direction of future development and advancements; ways to use these engineering proteins for therapeutic and vaccine applications; and the use of modified protein nanocarriers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Therapeutic Proteins: Methods and Protocols is a valuable resource for any researcher who are interested in learning more about the field of therapeutic proteins.Discovery, Production, and Conjugation of Therapeutic Proteins
Optimizing Cell-Free Protein Synthesis for Antimicrobial Protein Production
Design and Construction of Antibody Fusion Proteins Incorporating Variable New Antigen Receptor (VNAR) Domains
Selective and Site-Specific Incorporation of Nonstandard Amino Acids Within Proteins for Therapeutic Applications
An Approach for Antigen-Agnostic Identification of Virus-Like Particle-Displayed Epitopes that Engage Specific Antibody V Gene Regions
Split-Protein Therapeutic Platforms:​ Identifying Binder Pairs
Metal-Mediated Ligand Affinity Chemistry (MLAC)
Engineering Proteins for Therapeutic and Vaccine Applications
Recombinant Elastin-Based Bioelastomers for Biomedical Applications
Engineering Hepatitis B Virus (HBV) Protein Particles for Therapeutic Delivery
Design and Purification of Tag/​Catcher AP205-Based Capsid Virus-Like Particle Vaccines
Protein Nanocarriers Capable of Encapsulating Both Hydrophobic and Hydrophilic Drugs
Unnatural Amino Acid Engineering for Intracellular Delivery of Protein Therapeutics
Formulation of Chitosan–Zein Nano-in-Microparticles for Oral DNA Delivery
Engineering E2 Bionanoparticles​ for Targeted Delivery of Chemotherapeutic​s to Breast Cancer Cells
Constructing Nucleic Acid Delivering Lipoproteoplexes​ from Coiled-Coil Supercharged Protein and Cationic Liposomes
Cancer Cell Silicification and Surface Functionalizatio​n to Create Microbial Mimetic Cancer Vaccines
Melt Processing Virus-Like Particle-Based Vaccine Candidates into Biodegradable Polymer Implants