Barreiro Carlos, Barredo José-Luis (eds.) Microbial Steroids: Methods and Protocols

2nd edition. — Humana Press, 2023. — 351 p. — (Methods in Molecular Biology 2704). — ISBN13 9781071633847. — ISBN10 1071633848.This detailed volume explores experimental laboratory procedures for a wide range of steroid bioconversions. After an overview on the current trends and perspectives, the book continues with sections covering microbial screening and synthetic biology applied to microorganisms able to catabolize sterols, methods on strain characterization, including omics and biochemical analyses, methods of fermentation and biocatalysis for steroids production, as well as a chapter on the medical use of glucocorticoids in cancer patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Steroids: Methods and Protocols, Second Edition serves as an ideal reference source for laboratory and industrial professionals, as well as for students in a wide array of biological disciplines.General Overview
Current Trends and Perspectives in Microbial Bioconversions of Steroids
Microbial Screening and Genetic Manipulation
Isolation of Environmental Bacteria Able to Degrade Sterols and/or Bile Acids: Determination of Cholesterol Oxidase and Several Hydroxysteroid Dehydrogenase Activities in Rhodococcus, Gordonia, and Pseudomonas putida
Selection of Biodegrading Phytosterol Strains
Identification and Characterization of Some Genes, Enzymes, and Metabolic Intermediates Belonging to the Bile Acid Aerobic Catabolic Pathway from Pseudomonas
Targeted Mutagenesis of Mycobacterium Strains by Homologous Recombination
Genetic and Biochemical Analyses
RNA Preparation and RNA-Seq Bioinformatics for Comparative Transcriptomics
Bidimensional Analyses of the Intra- and Extracellular Proteomes of Steroid Producer Mycobacteria
Fungal Sterol Analyses by Gas Chromatography–Mass Spectrometry Using Different Derivatives
Cholesterol Assay Based on Recombinant Cholesterol Oxidase, ABTS, and Horseradish Peroxidase
Measuring the Interaction of Sterols and Steroids with Proteins by Microscale Thermophoresis
Fermentation and Biocatalysis for Steroids Production
Cultivation of Mycolicibacterium spp. Mutants in Miniaturized and High-Throughput Format to Characterize Their Growth, Phytosterol Conversion Ability, and Resistance to the Steroid Products
β-Sitosterol Bioconversion in Small-Scale Devices:​ From Microtiter Plates to Microfluidic Reactors
Biocatalysis of Steroids by Mycobacterium sp. in Aqueous and Organic Media
Scale-Up of Phytosterols Bioconversion into Androstenedione
Bioconversion of Phytosterols into Androstenedione by Mycolicibacterium
Selective Microbial Conversion of DHEA into 7α-OH-DHEA
Production of 11α-Hydroxysteroid Derivatives by Corynebacterium glutamicum Expressing the Rhizopus oryzae Hydroxylating System
Obtaining of 24-Norchol-4-ene-3,22-dione from Phytosterol with Mutants of Mycolicibacterium neoaurum
Stabilization of Enzymes by Using Thermophiles
Stigmasterol Removal by an Aerobic Treatment System
Steroids Application
Glucocorticoid Effect in Cancer Patients