Freiberg Alexander N., Rockx Barry (eds.) Nipah Virus: Methods and Protocols

Humana Press, 2023. — 297 p. — (Methods in Molecular Biology 2682). — ISBN13 9781071632826. — ISBN10 1071632825.This volume summarizes key methods and advancements in the field of henipavirus molecular biology. Chapters guide readers through protocols focusing on emerging bat-borne paramyxoviruses as well as pathogenicity caused by Nipah and Hendra viruses. Protocols are also included to help develop diagnostic tools, antiviral therapeutics, and vaccine creation. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Nipah Virus: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.Overview of Experimental Vaccines and Antiviral Therapeutics for Henipavirus Infection
Studying Henipaviruses Under Biosafety Level 2 Conditions
A Revised Diagnostic Quantitative RT-PCR for the Detection of Nipah Virus Infection
Recombinant Soluble Henipavirus Glycoprotein Preparation
Cell–Cell Fusion Assays to Study Henipavirus Entry and Evaluate Therapeutics
Studying Infectious Henipaviruses In Vitro
Recombinant Cedar Virus:​ A Henipavirus Reverse Genetics Platform
Utilizing Recombinant Reporter Henipaviruses to Conduct Antiviral Screening
In Vitro Antiviral Screening for Henipaviruses at BSL4
Isolation of Primary Porcine Bronchial Epithelial Cells for Nipah Virus Infections
Primary Culture of the Human Olfactory Neuroepithelium and Utilization for Henipavirus Infection In Vitro
Studying Infectious Henipaviruses In Vivo
Mouse Models of Henipavirus Infection
In Vivo Imaging of Nipah Virus Infection in Small Animal Rodent Models
Nonhuman Primate Models for Nipah and Hendra Virus Countermeasure Evaluation
Nipah Virus Aerosol Challenge of Three Distinct Particle Sizes in Nonhuman Primates
Generation and Characterization​ of a Humanized Lung Xenograft Mouse Model for Studying Henipavirus Pathogenesis
Ferret Models for Henipavirus Infection
Syrian Golden Hamster Model for Nipah Virus Infection
Immune Assays for Henipaviruses
Anti-Nipah Virus Enzyme-Linked Immunosorbent Assays with Non-human Primate and Hamster Serum
Assays for Detecting Henipavirus Antibodies
Host Responses to Henipavirus Infection
Profiling Host MicroRNA Responses to Henipavirus Infection
Host Transcriptome Analysis of Ferret Tissues Following Henipavirus Infection