Calderwood Stuart K. Prince Thomas L. (eds.) Chaperones: Methods and Protocols

2nd edition. — Humana Press, 2023 — 328 p. — (Methods in Molecular Biology 2693). — ISBN 978-1-0716-3341-0.This second edition volume expands on the previous edition with new discussions on the latest techniques used to study molecular chaperones and the stress response. The chapters in this book cover such as analysis of the initiation and regulation of the stress response; the role of heat shock protein 90 (Hsp90) in gene expression through chromosome-immunoprecipitation; features of chaperone function and biology; the emerging role of the extracellular HSPs; and the use of chaperones as biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Chaperones: Methods and Protocols, Second Edition is a valuable resource for all researchers who want to learn more about this interesting and developing field.Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter
Studying RNA Polymerase II Promoter-Proximal Pausing by In Vitro Immobilized Template and Transcription Assays
Role of Heat Shock Factors in Stress-Induced Transcription:​ An Update
A Workflow Guide to RNA-Seq Analysis of Chaperone Function and Beyond
Chromatin Immunoprecipitat​ion (ChIP) of Heat Shock Protein 90 (Hsp90)
Transfection and Thermotolerance Methods for Analysis of miR-570 Targeting the HSP Chaperone Network
Targeted Replacement of HSF1 Phosphorylation Sites at S303/​S307 with Alanine Residues in Mice Increases Cell Proliferation and Drug Resistance
Bimolecular Fluorescence Complementation Assay to Evaluate HSP90-Client Protein Interactions in Cells
Complementation Assays for Co-chaperone Function
Optimized Microscale Protein Aggregation Suppression Assay:​ A Method for Evaluating the Holdase Activity of Chaperones
Detecting Posttranslationa​l Modifications of Hsp90 Isoforms
Multiple Targeting of HSP Isoforms to Challenge Isoform Specificity and Compensatory Expression
Using a Modified Proximity Ligation Protocol to Study the Interaction Between Chaperones and Associated Proteins
Use of Native-PAGE for the Identification of Epichaperomes in Cell Lines
Molecular Chaperone Receptors:​ An Update
A Novel Heat Shock Protein 70-Based Vaccine Prepared from DC Tumor Fusion Cells:​ An Update
Methods to Assess the Impact of Hsp90 Chaperone Function on Extracellular Client MMP2 Activity
Proteomic Profiling of the Extracellular Vesicle Chaperone in Cancer
A Modified Differential Centrifugation Protocol for Isolation and Quantitation of Extracellular Heat Shock Protein 90 (eHsp90)
Immunohistochemi​stry of Human Hsp60 in Health and Disease:​ Recent Advances in Immunomorphology​ and Methods for Assessing the Chaperonin in Extracellular Vesicles
Multiplex Immunostaining Method to Distinguish HSP Isoforms in Cancer Tissue Specimens
Large-Scale Databases and Portals on Cancer Genome to Analyze Chaperone Genes Correlated to Patient Prognosis
Immunohistochemi​cal, Flow Cytometric, and ELISA-Based Analyses of Intracellular, Membrane-Expressed, and Extracellular Hsp70 as Cancer Biomarkers