Riechmann José Luis, Ferrándiz Cristina (eds.) Flower Development: Methods and Protocols

2nd edition. — Humana Press, 2023 — 586 p. — (Methods in Molecular Biology 2686). — ISBN 978-1-0716-3298-7.This second edition details new and updated protocols for experimental approaches that are currently used to study the formation of flowers. Chapters guide readers on genetic methods, phenotypic analyses, genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Flower Development: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.Review and Overview Chapters
Flower Development in Arabidopsis
Flower Development in the Solanaceae
The ABC of Flower Development in Monocots:​ The Model of Rice Spikelet
Model Species to Investigate the Origin of Flowers
Hormones and Flower Development in Arabidopsis
Genetic and Phenotypic Analyses
Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors
Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development
Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice
Isolation of Meiocytes and Cytological Analyses of Male Meiotic Chromosomes in Soybean, Lettuce, and Maize
Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis
Genetic and Phenotypic Analysis of Ovule Development in Arabidopsis
Experimental Systems
Floral Induction Systems for the Study of Arabidopsis Flower Development
Protoplasting and Fluorescence-Activated Cell Sorting of the Shoot Apical Meristem Cell Types
Protoplast Isolation for Plant Single-Cell RNA-seq
Plant Nuclei Isolation for Single-Nucleus RNA Sequencing
Isolation of Nuclei Tagged in Specific Cell Types (INTACT) in Arabidopsis
Molecular Biology, Genomics, and Systems Biology
RNA In Situ Hybridization on Plant Tissue Sections: Expression Analysis at Cellular Resolution
The GUS Reporter System in Flower Development Studies
Expression and Functional Studies of Leaf, Floral, and Fruit Developmental Genes in Non-model Species
Gene Expression Analysis by Quantitative Real-Time PCR for Floral Tissues
Misexpression Approaches for the Manipulation of Flower Development
Genomic Approaches for the Study of Flower Development in Floriculture Crops
Multi-Omics Methods Applied to Flower Development
Peptidomics Methods Applied to the Study of Flower Development
Quantifying Gene Expression Domains in Plant Shoot Apical Meristems
A NanoLuc-Based Transactivation Assay in Plants
A Hands-On Guide to Generate Spatial Gene Expression Profiles by Integrating scRNA-seq and 3D-Reconstructed Microscope-Based Plant Structures