Hoffmann Michèle J., Gaisa Nadine T., Nawroth R., Ecke Thorsten H. (eds.) Urothelial Carcinoma: Methods and Protocols

2nd edition. — Humana Press, 2023. — 321 p. — (Methods in Molecular Biology 2684). — ISBN 978-1-0716-3291-8.This fully updated volume explores recently improved avenues to study urothelial carcinomas. Beginning with several novel chapters on molecular characterization and urothelial carcinogenesis, the book continues with sections on cellular and animal models, biomarkers, and approaches for targeted therapy. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Urothelial Carcinoma: Methods and Protocols, Second Edition serves as a valuable resource to further increase our knowledge on urothelial carcinoma and also to aid research on numerous other cancers.Molecular Characterization
Scoring Systems for Immunohistochemi​stry in Urothelial Carcinoma
A Panel-Based Method for the Reproduction of Distinct Molecular Subtype Classifications of Muscle-Invasive Urothelial Bladder Cancer
Analysis of Mutational Signatures Using the mutSignatures R Library
A Drug Repurposing Pipeline Based on Bladder Cancer Integrated Proteotranscript​omics Signatures
Characterization​ of Native COMPASS Complex in Urothelial Carcinoma Cells by Size Exclusion Chromatography
Urothelial Carcinogenesis
Reconstructing Phylogenetic Relationship in Bladder Cancer:​ A Methodological Overview
Using Sister Chromatid Exchange Assay to Detect Homologous Recombination Deficiency in Epigenetically Deregulated Urothelial Carcinoma Cells
Identification of STAG2-Mutant Bladder Cancers by Immunohistochemi​stry
Cellular and Animal Models
Genome-Wide CRISPR Screening for the Identification of Therapy Resistance-Associated Genes in Urothelial Carcinoma
Tissue Slice Culture and Analysis of Tumor-Associated Hyaluronan in Urothelial Carcinoma
Biomarkers
NGS-Based Tumor-Informed Analysis of Circulating Tumor DNA
Considering the Effects of Modern Point-of-Care Urine Biomarker Assays in Follow-Up of Patients with High-Risk Non-muscle-Invasive Bladder Cancer
Simplex Droplet Digital PCR Assays for the Detection of TERT Promoter Mutations in Urine Samples for the Non-invasive Diagnosis of Urothelial Cancer
Predictive Biomarkers of Response to Neoadjuvant Therapy in Muscle Invasive Bladder Cancer
Assessment of PD-L1 Status in Urothelial Cancer
Therapy Development
Epigenetic Priming and Development of New Combination Therapy Approaches
Evaluation of FGFR Alteration Status in Urothelial Tumors
Antibody-Drug-Conjugates (ADC):​ A Novel Treatment Option in Urothelial Carcinoma
Intravesical Infusion of Oncolytic Virus CG0070 in the Treatment of Bladder Cancer
Analysis of ICAM-1 Expression on Bladder Carcinoma Cell Lines and Infectivity and Oncolysis by Coxsackie Virus A21