Ertl Peter, Rothbauer Mario (eds.) Cell-Based Microarrays: Methods and Protocols

Humana Press, 2018. — 258 p. — (Methods in Molecular Biology 1771). — ISBN 978-1-4939-7791-8.This volume provides a comprehensive look at the current fabrication methods, materials, cell patterning techniques, and practical application of live-cell microarrays. The chapters in this book are categorized into six parts: introduction, biointerface engineering and live-cell microarrays, printing techniques for live-cell microarrays, live-cell microarray application, microfluidic live-cell arrays, and advanced materials suitable for live-cell microarrays. Some of the topics covered are artificial micro-niche array with spatially structured biochemical cues; inkjet printing for biomedical application; fabrication of multi-electrode arrays for neurobiology applications; rapid prototyping of thermoplastic microfluidic devices; and oxygen-generating photocrosslinkable hydrogel. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Cell-Based Microarrays: Methods and Protocols is a valuable resource for anyone interested in expanding their knowledge and research of cell-based microarrays.Introduction
Next-Generation Live-Cell Microarray Technologies
Biointerface Engineering and Live-Cell Microarrays
Cell-Based Microarrays Using Superhydrophobic​ Platforms Patterned with Wettable Regions
3D Plasma Nanotextured Polymeric Surfaces for Protein or Antibody Arrays, and Biomolecule and Cell Patterning
Single Cell Microarrays Fabricated by Microscale Plasma-Initiated Protein Patterning (μPIPP)
Artificial Microniche Array with Spatially Structured Biochemical Cues
Printing Techniques for Live-Cell Microarrays
Three-Dimensional Cell-Based Microarrays:​ Printing Pluripotent Stem Cells into 3D Microenvironment​s
Fabrication of Biomolecule Microarrays for Cell Immobilization Using Automated Microcontact Printing
Hydrogel Printing Based on UV-Induced Projection for Cell-Based Microarray Fabrication
Inkjet Printing for Biomedical Applications
Live-Cell Microarray Application
High-Throughput Screening of Senescence Markers in Hematopoietic Stem Cells Derived from Induced Pluripotent Stem Cells
Primary and Stem Cell Microarrays:​ Application as Miniaturized Biotesting Systems
Fabrication of Multielectrode Arrays for Neurobiology Applications
Microfluidic Live-Cell Arrays
Rapid Prototyping of Thermoplastic Microfluidic Devices
Fabrication of Biomolecule Microarrays Using Rapid Photochemical Surface Patterning in Thiol–Ene-Based Microfluidic Devices
Fabrication and Operation of Microfluidic Hanging-Drop Networks
A Microfluidic Droplet Array System for Cell-Based Drug Combination Screening
Establishment of Microfluidic Spheroid Cultures for Biomedical Applications
Capillary Pinning Assisted Patterning of Cell-Laden Hydrogel Microarrays in Microchips
Advanced Materials Suitable for Live-Cell Microarrays
Oxygen-Generating Photocrosslinkab​le Hydrogel
A Self-Assembled Antifouling Nano-Biointerface for the Generation of Spheroids