Singleton Teresa L. (ed.) Schizosaccharomyces pombe: Methods and Protocols

Humana Press, 2018. — 239 p. — (Methods in Molecular Biology 1721). — ISBN 978-1-4939-7545-7.This volume presents various laboratory protocols, reviews, specific techniques and applications related to Schizosaccharomyces pombe. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Schizosaccharomyces pombe: Methods and Protocols hopes to serve as an excellent resource for faculty researchers, undergraduate student researchers, graduate researchers, government, and the medical community.Preparation of Solutions and Reagents
Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale Using DNA Combing
Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe
Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs
Molecular Cloning and Characterization​ of Small Viral Genome in Fission Yeast
Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast
Analysis of Reverse Transcribed mRNA Using PCR and Polyacrylamide Gel Electrophoresis
The No-Nonsens SDS-PAGE
Crystallization of Recombinant α-Actinin and Related Proteins
Estimation of GFP-Nucleoporin Amount Based on Fluorescence Microscopy
Antibody Pull-Down Experiments in Fission Yeast
Preparation of Cell Lysates of Fission Yeast for Immunoprecipitat​ion
Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo
In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe
High-Frequency Lithium Acetate Transformation of Schizosaccharomyces pombe
Tetrad Dissection in Fission Yeast
Random Spore Analysis in Fission Yeast
Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains
Schizosaccharomyces pombe Biotechnological Applications in Winemaking
Schizosaccharomyces pombe Isolation Protocol