Kim Kyeong Kyu, Subramani Vinod Kumar (eds.) Z-DNA: Methods and Protocols

Humana Press, 2023. — 333 p. — (Methods in Molecular Biology 2651). — ISBN 978-1-0716-3083-9.This detailed volume compiles key methods and techniques used to establish some of the structural and functional aspects of Z-form nucleic acids. Beginning with chapters on characterizing Z-DNA, the book continues by exploring structure determination techniques, gene- and genome-targeting methods and genome-wide approaches, Z-RNA study, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Z-DNA: Methods and Protocols serves as an ideal guide for students and researchers curious about this unique Z nucleic acid as an area of investigation, aiming to help the field grow, flourish, and unravel the hidden and novel roles of Z.The Origin of Left-Handed Poly[d(G-C)]
Characterization​ of Z-DNA Using Circular Dichroism
Characterization​ of Z-DNA by Infrared Spectroscopy
Crystallization of Z-DNA in Complex with Chemical and Z-DNA Binding Z-Alpha Protein
NMR Titration Studies in Z-DNA Dynamics
Single-Molecule Methods to Study Z-DNA Mechanics and Dynamics
BZ Junctions and Its Application as Probe (2AP) to Detect Z-DNA Formation and Its Effector
Oligonucleotide Containing 8-Trifluoromethyl-2′-Deoxyguanosine as a Z-DNA Probe
Chiroptical Properties of Z-DNA Using Ionic Porphyrins and Metalloporphyrin​s
Construction of a Z-DNA-Specific Recombinant Nuclease Zαα-FOK for Conformation Studies
Human Heme Oxygenase-1 Promoter Activity Is Mediated by Z-DNA Formation
ChIP-Seq Strategy to Identify Z-DNA-Forming Sequences in the Human Genome
Detection of Z-DNA Structures in Supercoiled Genome
Thermogenomic Analysis of Left-Handed Z-DNA Propensities in Genomes
DeepZ:​ A Deep Learning Approach for Z-DNA Prediction
Methods to Study Z-DNA-Induced Genetic Instability
Single-Molecule Visualization of B–Z Transition in DNA Origami Using High-Speed AFM
Adoption of A–Z Junctions in RNAs by Binding of Zα Domains
Detecting Z-RNA and Z-DNA in Mammalian Cells
Identification of ADAR1 p150 and p110 Associated Edit Sites
Z-DNA and Z-RNA:​ Methods—Past and Future