Hussey P.J., Wang P. (eds.) The Plant Cytoskeleton: Methods and Protocols

New York: Humana Press, 2023. — 368 p.This detailed volume explores the development of technologies and protocols that are currently being used to understand the nature and activities of the plant cytoskeleton. A focus for many of the chapters is on sample preparation, as the quality of plant organ/tissue preparation, from single to multicellular samples, determines the quality of the data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Plant Cytoskeleton: Methods and Protocols serves as an ideal guide for researchers interested in or starting to be interested in plant cell and molecular biology research.Preface
Contributors
Quantification of Microtubule-Bundling Activity of MAPs Using TIRF Microscopy
Actin: Static and Dynamic Studies
3D Visualization of Microtubules in Epidermal Pavement Cells
Quantitative Analysis of Microtubule Organization in Leaf Epidermis Pavement Cells
Single-Cell Confinement Methods to Study Plant Cytoskeleton
Documentation of Microtubule Collisions with Myosin VIII ATM1 Containing Membrane-Associated Structures
Imaging the Plant Cytoskeleton by High-Pressure Freezing and Electron Tomography
Confocal Microscopic Assays of Mitotically Active Proteins in an Agrobacterial Infiltration-Based, Cell Division-En...
Assessment of Spindle Shape Control by Spindle Poleward Flux Measurements and FRAP Bulk Analysis
Expansion Microscopy of Plant Cells (PlantExM)
Microfluidic Device for High-Resolution Cytoskeleton Imaging and Washout Assays in Physcomitrium (Physcomitrella)...
Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens
Gaining Insight into Large Gene Families with the Aid of Bioinformatic Tools
Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata
Studying Nuclear Dynamics in Response to Actin Disruption in Planta
Cytoskeleton Remodeling in Arabidopsis Stigmatic Cells Following Pollination
Investigation of ROP GTPase Activity and Cytoskeleton Dynamics During Tip Growth in Root Hairs and Pollen Tubes
Functional Analysis of Phospholipid Signaling and Actin Dynamics: The Use of Apical Growing Tobacco Pollen Tubes i...
Microtubule Reorganization During ABA-Induced Stomatal Closure in Arabidopsis
Imaging of Cortical Microtubules in Plants Under Salt Stress
Analysis of Actin Array Rearrangement During the Plant Response to Bacterial Stimuli
Live-Cell Imaging of Cytoskeletal Responses and Trafficking During Fungal Elicitation
Visualization and Quantification of the Dynamics of Actin Filaments in Arabidopsis Pollen Tubes
Noninvasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings
Visualization of Cytoskeleton Organization and Dynamics in Elongating Cotton Fibers by Live-Cell Imaging
Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells
Studying the Organization of the Actin Cytoskeleton in the Multicellular Trichomes of Tomato
Light Microscopy Technologies and the Plant Cytoskeleton
Investigating Plant Protein-Protein Interactions Using FRET-FLIM with a Focus on the Actin Cytoskeleton
Index