Dobnik David, Gruden K., Ramšak Živa, Coll A. (eds.) Solanum tuberosum: Methods and Protocols

Humana Press, 2021 — 180p. — (Methods in Molecular Biology 2354). — ISBN 978-1-0716-1608-6.This volume offers a comprehensive collection of experimental approaches to study Solanum tuberosum (potato). Chapters are divided into five sections covering a general overview of potato, the research fields of molecular biology, omics approaches, and bioinformatics, and finally, the application of the generated knowledge for crop improvement. Written in the format of the highly successful Methods in Molecular Biology series, each wet-lab chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and practical, Solanum tuberosum: Methods and Protocols aims to provide a collection of methods for the potato research community that will enable scientists gain insight into the world of the potato.History, Breeding and Long-term Storage
Importance of Potato as a Crop and Practical Approaches to Potato Breeding
Cryopreservation of Potato Shoot Tips for Long-Term Storage
High-throughput Omics Techniques
RNA Sequencing Analyses for Deciphering Potato Molecular Responses
Yeast Two-Hybrid Screening for Identification of Protein-Protein Interactions in Solanum tuberosum
Potato as a Model for Field Trials with Modified Gene Functions in Research and Translational Experiments
DAP-Seq Identification of Transcription Factor-Binding Sites in Potato
Mass Spectrometric Monitoring of Plant Hormone Cross Talk During Biotic Stress Responses in Potato (Solanum tuberosum L.)
A Comprehensive Guide to Potato Transcriptome Assembly
Bioinformatics and In Silico Approaches
MapMan Visualization of RNA-Seq Data Using Mercator4 Functional Annotations
Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq)
Methodologies for Discovery and Quantitative Profiling of sRNAs in Potato
Co-expression for Genotype-Phenotype Function Annotation in Potato Research
Computer Vision and Less Complex Image Analyses to Monitor Potato Traits in Fields
Molecular Biology Approaches
Quantifying the Contribution to Virulence of Phytophthora infestans Effectors in Potato
Identification of Solanum Immune Receptors by Bulked Segregant RNA-Seq and High-Throughput Recombinant Screening
Gene Editing in Potato Using CRISPR-Cas9 Technology
Gene Downregulation in Potato Roots Using Agrobacterium rhizogenes-Mediated Transformation
Crop Improvement and Diagnostic Assays
Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato
Toward the Design of Potato Tolerant to Abiotic Stress
Rapid Loop-Mediated Isothermal Amplification for Detection of the Ralstonia solanacearum Species Complex Bacteria in Symptomatic Potato Tubers and Plants