Fendt S.-M., Lunt S.Y. (eds.) Metabolic Signaling: Methods and Protocols

New York: Humana Press, 2019. — 298 p.This book provides protocols to quantify metabolism, to identify metabolic crosstalk, and to setup and develop tools and models to gain insight into metabolic signaling using experimental and computational approaches. Chapters detail protocols to quantify metabolism, identify metabolic crosstalk, and develop tools and models to gain a systems-level insight into metabolic signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Metabolic Signaling: Methods and Protocols aims to provide researchers with methods to study, perturb, and functionally interpret metabolism and metabolic signaling from the sub-cellular to the whole-body level.A Protocol to Compare Methods for Untargeted Metabolomics
High-Throughput Measure of Bioactive Lipids Using Non-targeted Mass Spectrometry
Measuring the Nutrient Metabolism of Adherent Cells in Culture
13C Tracer Analysis and Metabolomics in 3D Cultured Cancer Cells
Measuring In Vivo Tissue Metabolism Using 13C Glucose Infusions in Mice
Measuring Human Lipid Metabolism Using Deuterium Labeling: In Vivo and In Vitro Protocols
Measuring Rates of ATP Synthesis
Direct Estimation of Metabolic Flux by Heavy Isotope Labeling Simultaneous with Pathway Inhibition: Metabolic Flux Inhibition Assay
Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells Using Radioactive Tracers
Determining Compartment-Specific Metabolic Fluxes
Determining the Impact of Metabolic Nutrients on Autophagy
Measuring the Activation of Cell Death Pathways upon Inhibition of Metabolism
Determining Macrophage Polarization upon Metabolic Perturbation
Assessing the Impact of the Nutrient Microenvironment on the Metabolism of Effector CD8+ T Cells
Development of Patient-Derived Tumor Xenograft Models
Imaging Glioma Progression by Intravital Microscopy
Lipectomizing Mice for Applications in Metabolism
Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Culture Supernatants
Determining the Intracellular Organization of Organelles
The Fundamentals of Constructing and Interpreting Heat Maps