Bosselut R., Vacchio M.S. (eds.) T-Cell Development: Methods and Protocols

2nd edition. — Humana Press, 2023. — 382 p. — (Methods in Molecular Biology 2580). — ISBN 978-1-0716-2739-6.This second edition volume provides new and updated chapters detailing simple and accessible experiment protocols to explore thymus biology. Chapters are divided into three parts presenting short reviews, analysis strategies, protocols for cell preparation, flow cytometry analyses, Innate Lymphoid Cells (ILC), mouse T-cell development, antigen receptor-less cousins of T cells, bone marrow chimeras, thymic stroma, and multiple aspects of thymocyte biology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, T-Cell Development: Methods and Protocols, Second Edition aims to be a useful practical guide to help readers overcome obstacles associated with experimental approaches of T-cell development.Background Information
A Beginner’s Guide to T Cell Development
Revelations in Thymic Epithelial Cell Biology and Heterogeneity from Single-Cell RNA Sequencing and Lineage Tracing Methodologies
Early Development of Innate Lymphoid Cells
Development of γδ T Cells:​ Soldiers on the Front Lines of Immune Battles
Principles of Advanced Flow Cytometry:​ A Practical Guide
Core Approaches and Strategies
Genetic Strategies to Study T Cell Development
A Simplified Approach to Evaluating T Cell Development by Flow Cytometry
Purification of Thymocyte and T Cell Subsets
Generation of Bone Marrow Chimeras
Generation of Murine T Cell Effector Populations In Vitro
Specific Techniques
Large-Scale Isolation of Mouse Thymic Epithelial Cells
Generation of Retrogenic Mice to Investigate T Cell Development
Purification of Bone Marrow Precursors to T Cells and ILCs
Studying T Cell Development in Neonatal and Adult Thymic Slices
Induction of Human T Cell Development In Vitro with OP9-DL4-7FS Cells Expressing Human Cytokines
Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements
Ultrasound-Guided Intra-thymic Cell Injection
Fetal Thymic Organ Culture and Negative Selection
In Vitro Analysis of Thymocyte Signaling
Identification and Purification of Human T Cell Precursors
In Vitro Model Systems to Study Human T Cell Development
Zebrafish:​ A Tractable Model for Analysis of T Cell Development