Maccarrone Mauro (ed.) Endocannabinoid Signaling: Methods and Protocols

2nd edition. — Humana Press, 2023. — 502 p. — (Methods in Molecular Biology 2576). — ISBN 978-1-0716-2727-3.This second edition provides new and updated chapters detailing all major elements of the ECB system. Chapters guide readers through identification of drug targets, electrophysiology, computational chemistry, and machine learning. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Endocannabinoid Signaling: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about this important and developing field.Need for Methods to Investigate Endocannabinoid Signaling
Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues
Measuring the Content of Endocannabinoid-Like Compounds in Biological Fluids: A Critical Overview of Sample Preparation Methodologies
LC–MS/MS Analysis of AEA and 2-AG
Analysis of Omega-3 Fatty Acid-Derived N-Acylethanolamines in Biological Matrices
Endocannabinoid-Binding Receptors as Drug Targets
Assay of CB1 Receptor Binding
Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
Fluorescence-Based Assay for TRPV1 Channels
DNA–Protein-Interaction (DPI)-ELISA Assay for PPAR-γ Receptor Binding
Scintillation Proximity Assay (SPA)-Based Radioligand Binding for PPARα, PPARγ, and PPARδ Receptors
Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Cofactor Recruitment Assay for PPARα and PPARγ
Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
Assay of GTPγS Binding in Autoradiography
Cellular Assay to Study β-Arrestin Recruitment by the Cannabinoid Receptors 1 and 2
Endocannabinoid Metabolism and Transport as Drug Targets
Assay of NAT Activity
Radiometric Assay of NAPE-PLD Activity
Fluorescence-Based NAPE-PLD Activity Assay
Radiometric Assay of FAAH Activity
Fluorimetric Assay of FAAH Activity
Assay of NAAA Activity
Assay of DAGLα/β Activity
Assay of Monoacylglycerol Lipase Activity
Radiometric Assay of ABHD2 Activity
Oxygenation of Anandamide by Lipoxygenases