Pelus Louis M., Hoggatt Jonathan (eds.) Hematopoietic Stem Cells: Methods and Protocols

Humana Press, 2021. — 284 p. — (Methods in Molecular Biology 2567). — ISBN 978-1-0716-2678-8.This detailed volume collects updates on the technical advances in hematopoietic stem cell research and incorporates new techniques focused on the molecular/genetic, cellular, and whole organism levels. Exploring methods that apply stress to hematopoiesis, the book also contains chapters focused on better understanding the role of hematopoietic niches and their cellular components, as well as in vivo models that test and quantitate stem cell function and are key to further development of therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Hematopoietic Stem Cells: Methods and Protocols serves as a valued addition to laboratories focused on understanding hematopoietic stem cell biology and the therapeutic advances that can be derived from it.Overview
The Factory of Blood Production: Hematopoietic Stem Cells
Molecular Modification and Evaluation
Detection of DNA Damage in Hematopoietic Stem Cells
Evaluating Histone Acetylation in Mouse Hematopoietic Stem and Progenitor Cells Using Chromatin Immunoprecipitation
CRISPR Gene Editing of Hematopoietic Stem and Progenitor Cells
Lentiviral Transduction of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
Cellular Isolation and Characterization
Identification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
Isolation and Characterization of Fetal Liver Hematopoietic Stem Cells
Utilizing CyTOF to Examine Hematopoietic Stem and Progenitor Phenotype
Hematopoietic Stem Cell Identification Postirradiation
The Hematopoietic Niche and Accessory Cells
Intravital Microscopy for Hematopoietic Studies
Laser Micromachining of Bone as a Tool for Studying Bone Marrow Biology
MSC and HSPC Coculture: Mimicking Ex Vivo Bone Marrow Niche
Isolation of Thymus Stromal Cells from Human and Murine Tissue
Transplantation and In Vivo Models
Experimental Models of Mouse and Human Hematopoietic Stem Cell Transplantation
Hematopoietic Stem and Progenitor Cell Identification and Transplantation in Zebrafish
Establishing a Murine Model of the Hematopoietic Acute Radiation Syndrome
Purinergic Signaling and Its Role in Mobilization of Bone Marrow Stem Cells