Krämer Oliver H. (ed.) HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols

2nd edition. — Humana Press, 2023. — 508 p. — (Methods in Molecular Biology 2589). — ISBN 978-1-0716-2787-7.This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for novel and improved inhibitors of these enzymes, as reflected in this collection. Expert contributors explore the generation and evaluation of novel histone deacetylase inhibitors and new and improved techniques to assess acetylation-dependent molecular mechanisms in vitro and in vivo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to further elucidate this vital area of study.In Vivo and 3R Models to Assess HDAC Functions and Effects of HDACi
Novel HDAC Inhibitors and Improved Formulations for HDAC Inhibitors
Innovative Techniques to Determine the Impact of Acetylation on Chromatin, DNA Damage, and Cell Stress Responses
Modern Assays to Evaluate HDAC Functions and HDACi, Including CRISPR-Cas9 Techniques