Marques dos Reis Emily, Berti Fernanda (eds.) Vasculogenic Mimicry: Methods and Protocols

Humana Press, 2022. — 179 p. — (Methods in Molecular Biology 2514). — ISBN 978-1-0716-2402-9.This volume provides detailed protocols for the identification and understanding of vasculogenic mimicry process in vitro and in vivo, in addition to protocols for microscopy and histology. Chapters guide readers through different materials, commercial and homemade scaffolds, Matrigel, cancer spheroids, 3D tissue constructs, vasculogenic processes, and mathematical model building. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Vasculogenic Mimicry: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.Vasculogenic Mimicry Background
Vasculogenic Mimicry—An Overview
In Vitro Models to Study Angiogenesis and Vasculature
Protocols for VM In Vitro
In Vitro Tube Formation Assays in Matrigel
Vasculogenic Mimicry in a 3D Model In Vitro
Cancer Cell Spheroids as a 3D Model for Exploring the Pathobiology of Vasculogenic Mimicry
A Three-Dimensional Culture-Based Assay to Detect Early Stages of Vasculogenic Mimicry in Ovarian Cancer Cells
Co-immunoprecipitat​ion of Protein Complexes from Different Subcellular Compartments in Vasculogenic Mimicry Studies
Protocols for VM In Vivo
In Vivo Models to Evaluate Antitumor Drugs Effect on Vasculogenic Mimicry
Patient-Derived Xenograft Models for Studying Vascular Mimicry in Melanoma In Vivo
VM Analysis
Histological Evaluation of Long-Term Collagen Type I Culture
Histochemical Staining of Vasculogenic Mimicry
Identification of Vasculogenic Mimicry in Histological Samples
Evaluation of Pharmaceutical Inhibition of Vasculogenic Mimicry In Vitro
Immunohistochemi​stry for VM Markers
Confocal Laser Microscopy for VM Analysis with DAPI and Phalloidin Staining
Building Mathematical Models for Vascular Growth and Inhibition