Tomar Namrata (ed.) Mitochondria: Methods and Protocols

Humana Press, 2022. — 432 p. — (Methods in Molecular Biology 2497). — ISBN 978-1-0716-2308-4.This volume details comprehensive protocols and methodologies to assess mitochondrial bioenergetics and dynamics in different tissues and cells involving health and pathological states. Chapters guide readers through methods for assessment of the energy metabolism including Oxygen Consumption Rate (OCR), mitochondrial membrane potential, and measuring mitochondrial Ca2+ handling, and ROS emission. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting, and systematic reproducible protocols. Authoritative and cutting-edge, Mitochondria: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.Fast Determination of Mitochondrial Metabolism and Respiratory Complex Activity in Permeabilized and Intact Cells
Unbiased Millivolts Assay of Mitochondrial Membrane Potential in Intact Cells
Rotenone Decreases Ischemia-Induced Injury by Inhibiting Mitochondrial Permeability Transition:​ A Study in Brain
Assessment of Mitochondrial Complex II and III Activity in Brain Sections:​ A Histoenzymologic​al Technique
Measurement of Mitochondrial (Dys)Function in Cellular Systems Using Electron Paramagnetic Resonance (EPR):​ Oxygen Consumption Rate and Superoxide Production
Mitochondrial Calcium Handling in Isolated Mitochondria from a Guinea Pig Heart
Characterizing the Electron Transport Chain:​ Structural Approach
Characterizing the Electron Transport Chain:​ Functional Approach Using Extracellular Flux Analyzer on Mouse Tissue Samples
Simultaneous Acquisition of Mitochondrial Calcium Retention Capacity and Swelling to Measure Permeability Transition Sensitivity
Measuring Mitochondrial Function:​ From Organelle to Organism
Mitochondrial Toxicity of Organic Arsenicals
Measurements of Mitochondrial Respiration in Intact Cells, Permeabilized Cells, and Isolated Tissue Mitochondria Using the Seahorse XF Analyzer
Monitoring Mitochondrial Morphology and Respiration in Doxorubicin-Induced Cardiomyopathy
Creation of Yeast Models for Evaluating the Pathogenicity of Mutations in the Human Mitochondrial Gene MT-ATP6 and Discovering Therapeutic Molecules
In Vivo Analysis of Mitochondrial Protein Synthesis in Saccharomyces cerevisiae Mitochondrial tRNA Mutants
Visualizing Mitochondrial Importability of a Protein Using the Yeast Bi-Genomic Mitochondrial-Split-GFP Strain and an Ordinary Fluorescence Microscope
Analysis of Mitochondrial Performance in Lymphocytes Using Fluorescent Lifetime Imaging Microscopy
Monitoring Mitochondrial Perturbations During Infection
Measuring the Mitochondrial Ubiquinone (Q) Pool Redox State in Isolated Respiring Mitochondria
Experimental Setup for Investigation of Acute Mitochondrial Oxygen Sensing in Primary Cells
Assessing the Redox Status of Mitochondria Through the NADH/FAD2+ Ratio in Intact Cells
Monitoring Mitochondrial Membrane Potential in Live Cells Using Time-Lapse Fluorescence Imaging
Investigating Mitochondrial Ca2+ Dynamics in Isolated Mitochondria and Intact Cells: Application of Fluorescent Dyes and Genetic Reporters
A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX
Analysis of Organization and Activity of Mitochondrial Respiratory Chain Complexes in Primary Fibroblasts Using Blue Native PAGE
Multiplexing Seahorse XFe24 and ImageXpress Nano Platforms for Comprehensive Evaluation of Mitochondrial Bioenergetic Profile and Neuronal Morphology
Computational Modeling of Mitochondria to Understand the Dynamics of Oxidative Stress