Turksen K., Nagy A. (eds.) Induced Pluripotent Stem (iPS) Cells: Methods and Protocols

2nd edition. — Humana Press, 2022 — 829 p. — (Methods in Molecular Biology 2454). — ISBN 978-1-0716-2118-9.This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols, Second Edition aims to arm stem cell biologists, both novice and expert, with invaluable protocols that are currently being used in various laboratories around the world.Human Induced Pluripotent Stem Cell (iPSC) Handling Protocols: Maintenance, Expansion, and Cryopreservation
Generation of Induced Pluripotent Stem Cells from Human Bone Marrow–Derived Mesenchymal Stem Cells
Analysis of Clonal Composition in Human iPSC and ESC and Derived 2D and 3D Differentiated Cultures
Culturing Human Pluripotent Stem Cells on Micropatterned Silicon Surfaces
Porcine iPSC Generation: Testing Different Protocols to a Successful Application
Efficient High-Density hiPSCs Expansion in Simple Dialysis Device
Expanding the Differentiation Potential of Already-Established Pluripotent Stem Cells
Generation of Quiescent Cardiac Fibroblasts Derived from Human Induced Pluripotent Stem Cells
Inductive Coculture Differentiation of Induced Pluripotent Stem Cells into Cardiomyocytes
3D Microwell Platform for Cardiomyocyte Differentiation of Human Pluripotent Stem Cells
Scalable Generation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Application of Human Induced Pluripotent Stem Cell Technology for Cardiovascular Regenerative Pharmacology
Efficient and Safe Method of Generating Induced Pluripotent Stem Cells from Human Skin Fibroblasts and Subsequent Differentiation into Functional Cardiomyocytes
An Optical-Flow-Based Method to Quantify Dynamic Behavior of Human Pluripotent Stem Cell-Derived Cardiomyocytes in Disease Modeling Platforms
Production of Innervated Skeletal Muscle Fibers Using Human Induced Pluripotent Stem Cells
Generation of Human Neural Progenitors from Blood Samples by Interrupted Reprogramming
Robust and Highly Efficient Protocol for Differentiation of Human Pluripotent Stem Cells into Mesenchymal Stem Cells
The Development of Tissue Engineering Scaffolds Using Matrix from iPS-Reprogrammed Fibroblasts
3D Organoid Culture Using Skin Keratinocytes Derived from Human Induced Pluripotent Stem Cells