Brown Steven A. (ed.) Circadian Clocks: Methods and Protocols

Humana Press, 2021. — 349 p. — (Methods in Molecular Biology 2130). — ISBN 978-1-0716-0380-2.This volume presents techniques used by researchers from all branches of biology to study daily changes at a molecular level in many physiological systems. The chapters are organized into three parts and cover topics such as measuring and modeling physiological and behavioral rhythms; genome-wide analyses in circadian biology, and imaging and manipulating brain clocks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Circadian Clocks: Methods and Protocols is a valuable tool for any researcher interested in learning more about this developing field.Measuring and Modeling Physiological and Behavioral Rhythms
Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock
Inductively Coupled Plasma Mass Spectrometry for Elemental Analysis in Circadian Biology
The Assessment of Circadian Rhythms Within the Immune System
Measuring Circadian Rhythms in Human Cells
Measuring the Effects of Circadian Rhythm-Related Manipulations on Depression-Like Behavior in Rodents: Forced Swim and Tail Suspension Tests
Asking the Clock: How to Use Information from Questionnaires for Circadian Phenotyping
Simple Kinetic Models in Molecular Chronobiology
Genome-Wide Analyses in Circadian Biology
Searching Novel Clock Genes Using RNAi-Based Screening
Chromatin Immunoprecipitation (ChIP) from Mouse Liver Nuclei
Using GRO-Seq to Measure Circadian Transcription and Discover Circadian Enhancers
Circadian Metabolomics from Breath
A Lipidomics View of Circadian Biology
Circadian Lipidomics: Analysis of Lipid Metabolites Around the Clock
Phosphoproteome and Proteome Sample Preparation from Mouse Tissues for Circadian Analysis
Circadian Phosphorylation of CLOCK and BMAL1
Imaging and Manipulating Brain Clocks
Fluorescence Live Imaging of Drosophila Circadian Pacemaker Neurons
Light Input to the Mammalian Circadian Clock
Acute In Vivo Multielectrode Recordings from the Mouse Suprachiasmatic Nucleus
Perforated Multi-Electrode Array Recording in Hypothalamic Brain Slices
Collection of Mouse Brain Slices for Bioluminescence Imaging of Circadian Clock Networks
Computational Analysis of PER2::LUC Imaging Data
Electrophysiological Approaches to Studying the Suprachiasmatic Nucleus
Optogenetic Methods for the Study of Circadian Rhythms