Netherton Christopher L. (ed.) African Swine Fever Virus: Methods and Protocols

Humana Press, 2022. — 223 p. — (Methods in Molecular Biology 2503). — ISBN 978-1-0716-2332-9.This volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, African Swine Fever Virus: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.Working Safely with African Swine Fever Virus
Necropsy Procedures and Evaluation of Macroscopic Lesions of Pigs Infected with African Swine Fever Virus
Preparation of Immunofluorescen​tly Labeled Tissue Sections for Imaging at Low and High Magnifications in the Confocal Microscope
Primary Macrophage Culture from Porcine Blood and Lungs
Isolation of Porcine Bone Marrow Cells and Generation of Recombinant African Swine Fever Viruses
Laboratory Diagnosis and Quantification of African Swine Fever Virus Using Real-Time Polymerase Chain Reaction
Quantification of ASFV DNA and RNA in Ornithodoros Soft Ticks
Genotyping of African Swine Fever Virus
African Swine Fever Virus (ASFV) Indirect ELISA Test Based on the Use of the Soluble Cytoplasmic Semi- purified Antigen (ASFV CP-Ag)
Indirect Immunoperoxidase​ Test (IPT) for Detection of Antibodies Against African Swine Fever Virus (ASFV) on African Green Monkey Cell Lines (Vero, MS)
African Swine Fever Virus Hemadsorption Inhibition Assay
ELISpot Assay for the Detection of ASFV-Specific Interferon-Gamma (IFN-γ)-Producing Cells
Purification of African Swine Fever Virus
African Swine Fever Virus Plaque Assay and Disinfectant Testing
Bioorthogonal Labelling of African Swine Fever Virus-Infected Cells
Whole Genome Sequencing of African Swine Fever