Chu Justin Jang Hann, Ahidjo B.A., Mok Chee Keng (eds.) SARS-CoV-2: Methods and Protocols

Humana Press, 2022. — 1081 p. — (Methods in Molecular Biology 2452). — ISBN 978-1-0716-2111-0.This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, as well as bioinformatics and proteomics approaches in cellular response studies. This comprehensive collection also covers methods in immunology, animal models, antivirals and vaccine development strategies, as well as biorisk and mitigation measurements for SARS-CoV-2 research. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that is vital for success in the lab. Practical and timely, SARS-CoV-2: Methods and Protocols serves as an ideal guide for scientists investigating this prevalent and perilous RNA virus and the novel coronavirus disease that results from it.Clinical and Diagnostic Virology
Evolution and Epidemiology of SARS-CoV-2 Virus
Molecular Epidemiology of SARS-CoV-2 by Sequencing
Advanced Genetic Methodologies in Tracking Evolution and Spread of SARS-CoV-2
Antigen-Based Point of Care Testing (POCT) for Diagnosing SARS-CoV-2:​ Assessing Performance
Diagnostic Method for COVID-19 Using Sugar Chain–Immobilized Nanoparticles and Saliva Specimens
Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR Assay
Immunofluorescen​t Antibody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans
Cell Culture, Virus Replication, and Cellular Responses
Propagation and Quantification of SARS-CoV-2
Quantification of Infectious SARS-CoV-2 by the 50% Tissue Culture Infectious Dose Endpoint Dilution Assay
One-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
Profiling SARS-CoV-2 Infection by High-Throughput Shotgun Proteomics
Detection of SARS-CoV-2 by Mass Spectrometry
Bioinformatics-Based Approaches to Study Virus–Host Interactions During SARS-CoV-2 Infection
Human Nasal Epithelial Cells (hNECs) Generated by Air-Liquid Interface (ALI) Culture as a Model System for Studying the Pathogenesis of SARS-CoV-2
Immunology and Animal Models
Animal Models of COVID-19:​ Nonhuman Primates
Animal Models of COVID-19:​ Transgenic Mouse Model
Immunohistochemi​cal Detection of SARS-CoV-2 Antigens by Single and Multiple Immunohistochemi​stry
Measuring Neutralizing Antibodies to SARS-CoV-2 Using Lentiviral Spike-Pseudoviruses
Antivirals and Vaccines
Antiviral Strategies Against SARS-CoV-2:​ A Systems Biology Approach
Neutralization Assay for SARS-CoV-2 Infection:​ Plaque Reduction Neutralization Test
Pseudovirus-Based Assays for the Measurement of Antibody-Mediated Neutralization of SARS-CoV-2
Cytopathic Effect (CPE)-Based Drug Screening Assay for SARS-CoV-2
Biorisk and Mitigation Measurements
Strengthening Biorisk Management in Research Laboratories with Security-Sensitive Biological Agents Like SARS-CoV-2
Biorisk Management for SARS-CoV-2 Research in a Biosafety Level-3 Core Facility
Methods of SARS-CoV-2 Inactivation