Traxlmayr M.W. (ed.) Yeast Surface Display

Humana, 2022. — 642 p. — (Methods in Molecular Biology 2491). — ISBN-13 9781071622841.Поверхностный дисплейThis detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.Introduction to yeast surface display and its applications
Construction and selection of yeast surface display libraries and analysis of isolated variants
Selection of yeast surface display libraries for binding to mammalian cells or extracellular matrix
Specialized yeast surface display applications