Vagnoni Alessio (ed.) Axonal Transport: Methods and Protocols

Humana Press, 2022. — 568 p. — (Methods in Molecular Biology 2431). — ISBN 978-1-0716-1989-6.This volume covers a wide range of model systems from invertebrate to humans and in vitro and in vivo. Chapters detail methods on squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary neurons, c. elegans, and in vitro reconstitution assays. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Axonal Transport: Methods and Protocols aims to further our understanding of the trafficking of cellular components in the nervous system leading to the advancement of basic knowledge that underlines the cell biology of the neuron.Squid, Aplysia, and Xenopus
The Giant Axon of the Squid: A Simple System for Axonal Transport Studies
Live Imaging and Quantitative Analysis of Organelle Transport in Sensory Neurons of AplysiaCalifornica
Live Imaging of RNA Transport and Translation in Xenopus Retinal Axons
Mouse In Vivo and Ex Vivo
Imaging Axonal Transport in Ex Vivo Central and Peripheral Nerves
In Vivo Imaging of Axonal Organelle Transport in the Mouse Brain
Studying Axonal Transport in the Brain by Manganese-Enhanced Magnetic Resonance Imaging (MEMRI)
Rodent Primary Neurons
Microfluidic Neuromuscular Co-culture System for Tracking Cell-to-Cell Transfer and Axonal Transport of Labeled Proteins
Imaging Diversity in Slow Axonal Transport
Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses
Molecular Analysis of Axonal Transport Dynamics upon Modulation of Microtubule Acetylation
Live-Cell Imaging of RNA Transport in Axons of Cultured Primary Neurons
Visualizing Vesicle-Bound Kinesins in Cultured Hippocampal Neurons
Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons
Use of Microfluidics Chambers to Image Axonal transport in Adult Sensory Neurons
Human-Derived Neurons
High-Resolution Imaging of Mitochondria and Mitochondrial Nucleoids in Differentiated SH-SY5Y Cells
Assessment of Mitochondrial Trafficking as a Surrogate for Fast Axonal Transport in Human Induced Pluripotent Stem Cell–Derived Spinal Motor Neurons
Zebrafish
In Vivo Live Imaging of Axonal Transport in Developing Zebrafish Axons
Visualizing the Intracellular Trafficking in Zebrafish Mauthner Cells
Drosophila In Vivo, Ex Vivo, and Primary Neurons
Dissection and Direct Imaging of Axonal Transport in Drosophila Segmental Nerves
Detailed Imaging of Mitochondrial Transport and Precise Manipulation of Mitochondrial Function with Genetically Encoded Photosensitizers in Adult Drosophila Neurons
Mitochondrial DNA Transport in Drosophila Neurons
Live Imaging of Axonal Transport in the Adult Drosophila Central Nervous System
Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport
High-Resolution Live Imaging of Axonal RNP Granules in Drosophila Pupal Brain Explants
C. elegans
Analyzing the Impact of Gene Mutations on Axonal Transport in Caenorhabditis Elegans
Methods to Quantify and Relate Axonal Transport Defects to Changes in C. elegans Behavior
Imaging Intracellular Trafficking in Neurons of C. elegans
In vitro Reconstitution Assays
In Vitro Reconstitution of Molecular Motor-Driven Mitochondrial Transport
In Vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport