Eyster Kathleen M. (ed.) Estrogen Receptors: Methods and Protocols

2nd edition. — Humana Press, 2022. — 424 p. — (Methods in Molecular Biology 2418). — ISBN 978-1-0716-1919-3.This fully updated collection includes chapters on a wide array of techniques that are vital for advancing our understanding of the physiological and pathological effects of estrogen, mediated by estrogen receptor α (ERα) and estrogen receptor β (ERβ) as well as the non-genomic membrane-bound G-protein coupled estrogen receptor (GPER/GPR30). The protocols range from standard methods and important laboratory workhorses such as immunoblot and RT-qPCR, to newer technologies such as PET imaging, reporter animal models, polysome fractionation, and many others, involving a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written for this highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Estrogen Receptors: Methods and Protocols, Second Edition serves as an ideal guide to researchers working to explore the vibrancy and excitement of the field of estrogen receptors and estrogen actions.Antibody Validation for Estrogen Receptor Beta
Immunoblot Detection of the Phosphorylation of the Estrogen Receptor α as an Outcome of GPR30/GPER1 Activation
Immunoprecipitation Analyses of Estrogen Receptor α Phosphorylated at Serine 216 in the Mouse Liver
Immunofluorescent Verification of Silencing Estrogen Receptor α with siRNA in the Intact Rodent Brain
Detection and Functional Analysis of Estrogen Receptor α Phosphorylated at Serine 216 in Mouse Neutrophils
Quantification of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate in Response to Estrogen
In Situ Hybridization of Estrogen Receptors α and β in the Human Testis
Extraction of RNA and Analysis of Estrogen-Responsive Genes by RT-qPCR
PET Imaging of EstrogenReceptors Using 18F-Based Radioligands
The Use of ERE-Luc Reporter Mice to Monitor EstrogenReceptor Transcriptional Activity in a Spatio-Temporal Dimension
Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model
Assessing Estrogenic Activity of Classical EstrogenReceptor-Binding Compounds
An Optimized ChIP-Seq Protocol to Determine Chromatin Binding of EstrogenReceptor Beta
Polysome Fractionation for Transcriptome-Wide Studies of mRNA Translation
Anota2seq Analysis for Transcriptome-Wide Studies of mRNA Translation
Whole-Genome Genotyping Using DNA Microarrays for Population Genetics
Proteomics Analysis of the Estrogen Effects in the Rat Uterus Using Gel-LC and Tandem Mass Spectrometry Approaches
Expression Profiles of Estrogen-Regulated MicroRNAs in Cancer Cells
Molecular Cloning and Purification of the Protein Lysine Methyltransferase SMYD2 and its Co-crystallization with a Target Peptide from Estrogen Receptor Alpha