Vivanco Maria dM. (ed.) Mammary Stem Cells: Methods and Protocols

2nd edition. — Humana Press, 2022. — 353 p. — (Methods in Molecular Biology 2471). — ISBN 978-1-0716-2192-9.This second edition provides an overview of recent developments and approaches used by researchers to investigate the properties and functions of mammary epithelial and stem cells, which will contribute to understand the heterogeneity of the mammary gland and of breast cancer. Chapters detail processes used to characterize stem cells, single cell RNA sequencing, computational methods, sophisticated imaging techniques, and a variety of model systems, among others. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols, Second Edition aims to make available protocols used to navigate the intricate behavior of mammary stem cells and to gain further knowledge to take us closer to the design of innovative strategies to prevent and treat breast cancer.Protocol for Studying Embryonic Mammary Gland Branching Morphogenesis Ex Vivo
Multidimensional Fluorescence Imaging of Embryonic and Postnatal Mammary Gland Development
Single-Cell Transcriptomic and Epigenetic Analyses of Mouse Mammary Development Starting with the Embryo
Computational Methods to Identify Cell-Fate Determinants, Identity Transcription Factors, and Niche-Induced Signaling Pathways for Stem Cell Research
Differential Proteomic Analysis of Complex Mixtures by Label-Free nLC MS/MS
Orthotopic Transplantation of Mouse Mammary Epithelial Cells
Lineage Tracing Methods to Study Mammary Epithelial Hierarchies In Vivo
Lentiviral Transduction of Mammary Epithelial Cells
Modification of Single Cells Within Mouse Mammary Gland Derived Acini via Viral Transduction
Surgical Procedure for Implantation of Human Tumor Tissue into the Epithelium-Free Mammary Fat Pad of Immunocompromised Mice to Generate Patient-Derived Xenografts (PDX)
The Chicken Embryo Chorioallantoic Membrane (CAM): A Versatile Tool for the Study of Patient-Derived Xenografts
Intraductal Injections into the Mouse Mammary Gland
Modeling Breast Cancer in Organoid and Intraductal Models
Single Organoids Droplet-Based Staining Method for High-End 3D Imaging of Mammary Organoids
Divide and Conquer: Isolating Cell Populations to Investigate How Breast Cancer Risk Factors Alter the Breast Microenvironment
An Organotypic Assay to Study Epithelial-Fibroblast Interactions in Human Breast
Patient-Derived Explant Cultures of Normal and Tumor Human Breast Tissue
Negative-Selection Enrichment of Circulating Tumor Cells from Peripheral Blood Using the Microfluidic CTC-iChip
Measuring Mechanical Properties of Breast Cancer Cells with Atomic Force Microscopy
Breast Cancer Stem Cells: A Clinician’s View