Annunziato Francesco, Maggi Laura, Mazzoni Alessio (eds.) T-Helper Cells: Methods and Protocols

Humana Press, 2021. — 390 p. — (Methods in Molecular Biology 2285). — ISBN 978-1-0716-1310-8.The aim of this volume is to provide a comprehensive description of methods and protocols useful for the further study of T-helper cells. Chapters guide readers through T-helper cell recovery, molecular study, signal transduction pathways, T-cell manipulation and, last but not least, “omic” approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T- Helper Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.Human and Murine T-Helper Cell Recovery from Organs and Tissues
Identification and Purification of Human Memory T Helper Cells from Peripheral Blood
Transcription Factor Evaluation by Flow Cytometry
Mass Cytometry Analysis of T-Helper Cells
Murine T-Helper Cell Differentiation and Plasticity
Identification of Follicular T-Cell Subsets in Murine and Human Tissues
Analysis of T-Cells in Inflamed Nonlymphoid Tissues
TCR Vβ Evaluation by Flow Cytometry
Evaluation of Cytokine Production at the Single Cell Level by Flow Cytometry Upon Polyclonal Stimulation
Evaluation of Secreted Cytokines by Multiplex Bead-Based Assay (X MAP Technology, Luminex)
Quantifying Antigen-Specific T-Cells by Assessing Their Antigen-Induced Proliferation
Flow Cytometric Characterization of Human Antigen-Reactive T-Helper Cells
Identification of Human Antigen-Specific CD4+ Cells with Peptide-MHC Multimer Technologies
Human T-Cell Cloning by Limiting Dilution
Human Germinal Center Reaction: Assessing T Follicular Helper-B Cells Interaction In Vitro
Study of Signal Transduction Pathways by Phospho-Protein Evaluation
The Study of Protein–DNA Interactions in CD4+ T-Cells Using ChIPmentation
mRNA Transfection of T-Lymphocytes by Electroporation
Transduction of Human T Cell Subsets with Lentivirus
Plasmid- or Ribonucleoprotein-Mediated CRISPR/Cas Gene Editing in Primary Murine T Cells
Generation of Sequencing Libraries for Building Immune Cell Methylomes
Gene Expression Analysis of T-Cells by Single-Cell RNA-Seq
Quantifying Proteome and Protein Modifications in Activated T Cells by Multiplexed Isobaric Labeling Mass Spectrometry
Metabolomics, Lipidomics, and Immunometabolism
Experimental Mouse Models of Asthma and Analysis of CD4 T Cells
Murine T-Cell Transfer Colitis as a Model for Inflammatory Bowel Disease
Experimental Autoimmune Encephalomyelitis