McAllister Florencia (ed.) Cancer Immunoprevention: Methods and Protocols

Humana Press, 2022. — 226 p. — (Methods in Molecular Biology 2435). — ISBN 978-1-0716-2013-7.This volume provides methods and techniques to further the study of cancer immunoprevention. Chapters describe tumor-associated antigens, cancer immune-preventive vaccines, generation of TILs, development of monoclonal antibodies, immunoprofiling technologies, tissue multispectral imaging techniques, mass cytometry on suspensions, mutiparametric flow cytometry, genomic expression analysis, and proteomic profiling of tumor microenvironment cell populations and metabolic assessment through novel imaging technologies. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Mechanisms of Antitumor Immunity and Immunosurveillan​ce
Targets and Strategies for Cancer Immunoprevention​
Discovery of Targets for Cancer Immunoprevention​
Exosome-related Methods and Potential Use as Vaccines
Isolation and Maintenance of Tumor-Infiltrating Lymphocytes for Translational and Clinical Applications:​ Established Methods and New Developments
Monoclonal Antibodies Generation:​ Updates and Protocols on Hybridoma Technology
Transcriptomic-Assisted Immune and Neoantigen Profiling in Premalignancy
Assessment of the Murine Tumor Microenvironment​ by Multiplex Immunofluorescen​ce
Cytometry of Mass for Murine Immunoprofiling
Interrogating the Tumor Microenvironment​ by Deep Tissue Immunoprofiling
Proteomic Profiling of the Tumor Microenvironment​
Metabolic Imaging Using Hyperpolarizatio​n for Assessment of Premalignancy
Murine Models for Lineage Tracing Cancer Initiating Cells
Murine Model of Obesity-Induced Cancer
Lung Cancer Murine Models and Methodology for Immunopreventive​ Study
Mouse Models to Study Secondary Cancer Prevention