Kreeger Pamela K. (ed.) Ovarian Cancer: Methods and Protocols

Humana Press, 2022. — 321 p. — (Methods in Molecular Biology 2424). — ISBN 978-1-0716-1955-1.This detailed volume provides a robust set of methods to understand variation between patients with ovarian cancers, in vitro models to better study different stages of the disease, and in vivo models to test therapies. Beginning with clinical perspectives to orient basic scientists, the book continues with sections exploring methods to characterize features of the tumor microenvironment (TME), techniques to isolate cells for in vitro study, and in vitro and in vivo models to study the disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Ovarian Cancer: Methods and Protocols serves as an ideal guide for researchers seeking to improve their study of ovarian cancer and find new therapeutic approaches.Clinical Perspectives
Clinical Staging of Ovarian Cancer
Pathologic Classification of Ovarian Cancer
Tumor Microenvironment
Multiparameter Flow Cytometry for Detailed Characterization of Peritoneal Immune Cells from Patients with Ovarian Cancer
Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumors
Processing and Analysis of Ascites
Multispectral Staining and Analysis of Extracellular Matrix
Quantitative Analysis of the Extracellular Matrix by Immunoblot
Cell Isolation
Multiparameter Single-Cell Characterization of Ovarian Intratumor Heterogeneity
Culturing Primary Human Mesothelial Cells
Isolation of Normal and Cancer-Associated Fibroblasts
Isolation of Primary Normal and Cancer-Associated Adipocytes from the Omentum
Model Systems
Isolation of Fallopian Tube Epithelium for Assessment of Cilia Beating Frequency (CBF)
Ex Vivo Ovarian Culture to Model the Initial Metastasis in Ovarian Cancer
In Vivo and Ex Vivo Analysis of Omental Adhesion in Ovarian Cancer
The Role of the Tumor Microenvironment in CSC Enrichment and Chemoresistance: 3D Co-culture Methods
Model Systems
Establishment of In Vivo Ovarian Cancer Mouse Models Using Intraperitoneal Tumor Cell Injection
Humanized Patient-Derived Xenograft Models of Ovarian Cancer
Xenograft Models of Ovarian Cancer for Therapy Evaluation
Confocal Imaging of Single-Cell Signaling in Orthotopic Models of Ovarian Cancer